Information on EC 3.5.1.72 - D-benzoylarginine-4-nitroanilide amidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.72
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RECOMMENDED NAME
GeneOntology No.
D-benzoylarginine-4-nitroanilide amidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-benzoyl-D-arginine-4-nitroanilide + H2O = N-benzoyl-D-arginine + 4-nitroaniline
show the reaction diagram
stereospecific enzyme activity to D-isomer
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
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-
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SYSTEMATIC NAME
IUBMB Comments
N-benzoyl-D-arginine-4-nitroanilide amidohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
119345-26-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
found in all aerobic and anaerobic members of the family tested
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Manually annotated by BRENDA team
168 trpC2 and nonspore-forming mutant 43.4 spo0A43 leu-8
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Manually annotated by BRENDA team
var. israelensis
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Manually annotated by BRENDA team
DSM 2228
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Manually annotated by BRENDA team
marine aerobic sporeforming bacterium 6A10
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Manually annotated by BRENDA team
no activity in Acetobacter xylinum
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Manually annotated by BRENDA team
no activity in Corynebacterium bovis
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Manually annotated by BRENDA team
no activity in Escherichia coli
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Manually annotated by BRENDA team
no activity in Escherichia coli JM83
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Manually annotated by BRENDA team
no activity in Sporohalobacter lortetii
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Manually annotated by BRENDA team
no activity in Streptococcus mutans serotype C
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Manually annotated by BRENDA team
no activity in Streptococcus pneumoniae
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Manually annotated by BRENDA team
no activity in Vibrio costicola
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Manually annotated by BRENDA team
ATCC 35420
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg e + 4-nitroaniline
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetamide
leupeptin
N-benzoyl-D-Arg-4-nitroanilide
NaCl
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1.0 M and higher: inhibition, 100 mM: activation
p-chloromercuribenzene sulfonic acid
p-chloromercuribenzoate
tosyl-L-Arg methyl ester
tosyl-L-Lys chloromethyl ketone
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
dithiothreitol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029 - 0.14
N-benzoyl-D-Arg 4-nitroanilide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
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strain 168, subcellular distribution, no activity in cell wall lysate and membranes
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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strain 168, 2 min, 40% loss of activity in 0.05 M Tris buffer, pH 7.8, 60% loss of activity with 5 mM MgCl2, 80% loss of activity with 5 mM CaCl2
additional information
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strain 168, Ca2+ and Mg2+ ions decrease thermostability
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Ca2+ decreases thermostability
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Mg2+ decreases thermostability
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
strain 168, partial
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