Information on EC 3.5.1.65 - theanine hydrolase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.5.1.65
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RECOMMENDED NAME
GeneOntology No.
theanine hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N5-ethyl-L-glutamine + H2O = L-glutamate + ethylamine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
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-
-
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SYSTEMATIC NAME
IUBMB Comments
N5-ethyl-L-glutamine amidohydrolase
Also acts on other N-alkyl-L-glutamines.
CAS REGISTRY NUMBER
COMMENTARY hide
99533-51-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
T-1
-
-
Manually annotated by BRENDA team
T-1
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glutamine + H2O
D-glutamate + NH3
show the reaction diagram
-
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
show the reaction diagram
-
-
-
-
?
N5-ethyl-D-glutamine + H2O
D-glutamate + ethylamine
show the reaction diagram
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D-theanine, probably by the same enzyme like L-theanine
-
?
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
show the reaction diagram
N5-isobutyl-L-glutamine + H2O
L-glutamate + isobutylamine
show the reaction diagram
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gamma-glutamyl-isobutylamide, 136% of activity compared to L-theanine
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-
?
N5-methyl-L-glutamine + H2O
L-glutamate + methylamine
show the reaction diagram
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gamma-glutamylmethylamide, 77% of activity compared to L-theanine
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-
?
N5-n-butyl-L-glutamine + H2O
L-glutamate + n-butylamine
show the reaction diagram
-
gamma-glutamyl-n-butylamide, 276% of activity compared to L-theanine
-
-
?
N5-n-pentyl-L-glutamine + H2O
L-glutamate + n-pentylamine
show the reaction diagram
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gamma-glutamyl-n-amylamide, 211% of activity compared to L-theanine
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-
?
N5-n-propyl-L-glutamine + H2O
L-glutamate + n-propylamine
show the reaction diagram
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gamma-glutamyl-n-propylamide, 144% of activity compared to theanine
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-
?
theanine + hydroxylamine
gamma-glutamylhydroxamic acid + ethylamine
show the reaction diagram
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transferase reaction
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N5-ethyl-L-glutamine + H2O
L-glutamate + ethylamine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetic acid
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8 mM: 88% of activity compared to H2O
acidic amino acid
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-
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beta-Alanine
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partial inhibition, 8 mM: 89% of activity compared to H2O
CuSO4
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10 mM: strong inhibition
EDTA
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10 mM: 30% inhibition
glycine
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8 mM: 99% of activity compared to H2O
HgCl2
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1 mM: strong inhibition
iodoacetate
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2 mM: strong inhibition, protected partially, about 30%, by preincubation, 15 min, with 20 mM 2-mercaptoethanol
L-alanine
L-asparagine
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strong inhibition, 8 mM: 76% of activity compared to H2O
L-aspartic acid
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strong inhibition, 8 mM: 53% of activity compared to H2O
L-Citric acid
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8 mM: 95% of activity compared to H2O
L-Glutamic acid
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strong inhibition, 0.7 mM: 86%, 3.3 mM: 58%, 8 mM: 56%, 53.3 mM: 25% of activity compared to H2O
L-glutamine
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strong inhibition, 8 mM: 75% of activity compared to H2O
L-leucine
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partial inhibition, 8 mM: 89% of activity compared to H2O
L-serine
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partial inhibition, 8 mM: 95% of activity compared to H2O
Maleic acid
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8 mM: 98% of activity compared to H2O
oxaloacetic acid
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strong inhibition, 8 mM: 59% of activity compared to H2O
succinic acid
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strong inhibition, 8 mM: 77% of activity compared to H2O
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-glutamate
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activation and induction
L-glutamate
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activation and induction
L-Malic acid
L-Theanine
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activation and induction
additional information
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D-malic acid, 8 mM, no effect
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.2
D-Theanine
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partial purified enzyme
5.8
L-Theanine
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partial purified enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 10.5
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about half of maximum activity at pH 5.5 and 10.5
6.5 - 9
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pH 6.5: about 22% of maximal activity, pH 9.0: about 95% of maximal activity
7.5
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about half of maximum activity at pH 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9.5
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quite stable between
209221
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
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60 min, stable at over a wide range of pH; above 55°C: rapid inactivation of enzyme
60
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15 min, rapid inactivation of enzyme
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme very unstable
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theanine hydrolase activity increases slightly during first 10 h after plucking but thereafter decreased gradually
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 45% loss of activity within 1 day
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, glutaminase could not be separated