Information on EC 3.5.1.64 - Nalpha-benzyloxycarbonylleucine hydrolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.64
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RECOMMENDED NAME
GeneOntology No.
Nalpha-benzyloxycarbonylleucine hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Nalpha-benzyloxycarbonyl-L-leucine + H2O = benzyl alcohol + CO2 + L-leucine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
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SYSTEMATIC NAME
IUBMB Comments
Nalpha-benzyloxycarbonyl-L-leucine urethanehydrolase
Also acts on Nalpha-t-butoxycarbonyl-L-leucine, and, more slowly, on the corresponding derivatives of L-aspartate, L-methionine, L-glutamate and L-alanine. cf. EC 3.5.1.58 N-benzyloxycarbonylglycine hydrolase.
CAS REGISTRY NUMBER
COMMENTARY hide
100630-47-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Soil bacterium
strain F-96
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Manually annotated by BRENDA team
Soil bacterium F-96
strain F-96
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Nalpha-(4-aminobenzoyl)-L-Glu + H2O
1-amino-4-hydroxybenzene + CO2 + L-Glu
show the reaction diagram
Soil bacterium
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poor substrate
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-
?
Nalpha-(4-hydroxybenzoyl)-L-Asp + H2O
1,4-dihydroxybenzene + CO2 + L-Asp
show the reaction diagram
Soil bacterium
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poor substrate
-
-
?
Nalpha-acetyl-L-Asp + H2O
methanol + CO2 + L-Asp
show the reaction diagram
Soil bacterium
-
poor substrate
-
-
?
Nalpha-acetyl-L-Glu + H2O
methanol + CO2 + L-Glu
show the reaction diagram
Soil bacterium
-
poor substrate
-
-
?
Nalpha-acetyl-L-Leu + H2O
methanol + CO2 + L-Leu
show the reaction diagram
Soil bacterium
-
40% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis
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?
Nalpha-benzoyl-L-Glu + H2O
phenol + CO2 + L-Glu
show the reaction diagram
Soil bacterium
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-
-
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?
Nalpha-benzoyl-L-Leu + H2O
phenol + CO2 + L-Leu
show the reaction diagram
Soil bacterium
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-
-
-
?
Nalpha-benzyloxycarbonyl-Gly + H2O
benzyl alcohol + CO2 + Gly
show the reaction diagram
Soil bacterium
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poor substrate
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?
Nalpha-benzyloxycarbonyl-L-Ala + H2O
benzyl alcohol + CO2 + L-Ala
show the reaction diagram
Soil bacterium
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-
-
?
Nalpha-benzyloxycarbonyl-L-Asn + H2O
benzyl alcohol + CO2 + L-Asn
show the reaction diagram
Soil bacterium
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poor substrate
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?
Nalpha-benzyloxycarbonyl-L-Asp + H2O
benzyl alcohol + CO2 + L-Asp
show the reaction diagram
Soil bacterium
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-
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?
Nalpha-benzyloxycarbonyl-L-Cys + H2O
benzyl alcohol + CO2 + L-Cys
show the reaction diagram
Soil bacterium
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poor substrate
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?
Nalpha-benzyloxycarbonyl-L-Gln + H2O
benzyl alcohol + CO2 + L-Gln
show the reaction diagram
Soil bacterium
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poor substrate
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?
Nalpha-benzyloxycarbonyl-L-Glu + H2O
benzyl alcohol + CO2 + L-Glu
show the reaction diagram
Soil bacterium
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-
-
?
Nalpha-benzyloxycarbonyl-L-His + H2O
benzyl alcohol + CO2 + L-His
show the reaction diagram
Soil bacterium
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very poor substrate
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?
Nalpha-benzyloxycarbonyl-L-Ile + H2O
benzyl alcohol + CO2 + L-Ile
show the reaction diagram
Soil bacterium
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very poor substrate
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?
Nalpha-benzyloxycarbonyl-L-Met + H2O
benzyl alcohol + CO2 + L-Met
show the reaction diagram
Soil bacterium
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-
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?
Nalpha-benzyloxycarbonyl-L-Phe + H2O
benzyl alcohol + CO2 + L-Phe
show the reaction diagram
Soil bacterium
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very poor substrate
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?
Nalpha-benzyloxycarbonyl-L-Ser + H2O
benzyl alcohol + CO2 + L-Ser
show the reaction diagram
Soil bacterium
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poor substrate
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?
Nalpha-benzyloxycarbonyl-L-Thr + H2O
benzyl alcohol + CO2 + L-Thr
show the reaction diagram
Soil bacterium
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very poor substrate
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?
Nalpha-benzyloxycarbonyl-L-Tyr + H2O
benzyl alcohol + CO2 + L-Tyr
show the reaction diagram
Soil bacterium
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very poor substrate
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?
Nalpha-benzyloxycarbonyl-L-Val + H2O
benzyl alcohol + CO2 + L-Val
show the reaction diagram
Soil bacterium
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poor substrate
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?
Nalpha-benzyloxycarbonyl-Leu + H2O
benzyl alcohol + CO2 + L-Leu
show the reaction diagram
Soil bacterium
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best substrate
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?
Nalpha-tert-butoxycarbonyl-Gly + H2O
tert-butanol + CO2 + Gly
show the reaction diagram
Soil bacterium
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-
-
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?
Nalpha-tert-butoxycarbonyl-L-Ala + H2O
tert-butanol + CO2 + L-Ala
show the reaction diagram
Soil bacterium
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-
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?
Nalpha-tert-butoxycarbonyl-L-Asp + H2O
tert-butanol + CO2 + L-Asp
show the reaction diagram
Soil bacterium
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?
Nalpha-tert-butoxycarbonyl-L-Gln + H2O
tert-butanol + CO2 + L-Gln
show the reaction diagram
Soil bacterium
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poor substrate
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?
Nalpha-tert-butoxycarbonyl-L-Leu + H2O
tert-butanol + CO2 + L-Leu
show the reaction diagram
Soil bacterium
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80% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis
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?
Nalpha-tert-butoxycarbonyl-L-Met + H2O
tert-butanol + CO2 + L-Met
show the reaction diagram
Soil bacterium
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poor substrate
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?
Nalpha-tert-butoxycarbonyl-L-Val + H2O
tert-butanol + CO2 + L-Val
show the reaction diagram
Soil bacterium
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poor substrate
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
Soil bacterium
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required for activity, optimum concentration about 1 mM
Divalent cations
Soil bacterium
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required for activity
Zn2+
Soil bacterium
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required for activity, optimum concentration about 1 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
Soil bacterium
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1 mM: complete inhibition
iodoacetate
Soil bacterium
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1 mM: strong inhibition
p-chloromercuribenzoate
Soil bacterium
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1 mM: strong inhibition
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.1
Soil bacterium
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additional information
Soil bacterium
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pI: 3.31
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
Soil bacterium
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39000
Soil bacterium
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strain F-96, x * 39000, SDS-PAGE
116000
Soil bacterium
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strain F-96, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
Soil bacterium
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17 h, 37°C, stable at
172071
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
Soil bacterium
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17 h, pH 6-7, stable at
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
strain F-96
Soil bacterium
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