Information on EC 3.5.1.57 - tryptophanamidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.57
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RECOMMENDED NAME
GeneOntology No.
tryptophanamidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-tryptophanamide + H2O = L-tryptophan + NH3
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
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-
-
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SYSTEMATIC NAME
IUBMB Comments
L-tryptophanamide amidohydrolase
Requires Mn2+. Also acts on N-ethylformamide and L-tyrosinamide, and on some tryptophan dipeptides.
CAS REGISTRY NUMBER
COMMENTARY hide
76689-19-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glycine-beta-naphthylamide + H2O
glycine + 2-naphthylamine
show the reaction diagram
-
-
-
?
L-alaninamide + H2O
L-alanine + NH3
show the reaction diagram
L-asparagine + H2O
L-aspartate + NH3
show the reaction diagram
L-citrullin + H2O
?
show the reaction diagram
-
22% of the activity with L-tryptophanamide
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-
?
L-citrulline + H2O
?
show the reaction diagram
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hydrolyzed 22% as rapidly as L-tryptophanamide
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-
?
L-glutamine + H2O
L-glutamate + NH3
show the reaction diagram
L-leucinamide + H2O
L-leucine + NH3
show the reaction diagram
L-methioninamide + H2O
L-methionine + NH3
show the reaction diagram
L-phenylalaninamide + H2O
L-phenylalanine + NH3
show the reaction diagram
L-proline-beta-naphthylamide + H2O
L-proline + 2-naphthylamine
show the reaction diagram
-
-
-
?
L-serinamide + H2O
L-serine + NH3
show the reaction diagram
L-tryptophan-beta-naphthylamide + H2O
L-tryptophan + 2-naphthylamine
show the reaction diagram
-
-
-
?
L-tryptophanamide + H2O
L-tryptophan + NH3
show the reaction diagram
L-tyrosinamide + H2O
L-tyrosine + NH3
show the reaction diagram
L-tyrosine-beta-naphthylamide + H2O
L-Tyr + 2-naphthylamine
show the reaction diagram
-
-
-
?
L-valinamide + H2O
L-valine + NH3
show the reaction diagram
additional information
?
-
-
high affinity towards peptides having a L-Trp residue at the N-terminal moiety
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-tryptophanamide + H2O
L-tryptophan + NH3
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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weak
2,2'-dipyridyl
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monoiodoacetate
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weak
additional information
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no inhibition by high levels of the product L-tryptophan
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.6
L-Tryptophanamide
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9 - 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 45
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 60
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crystalline enzyme, 35°C: about 75% of activity maximum, 60°C: about 35% of activity maximum
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7
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isoelectrofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
hypocotyl transition zone
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68000
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4 * 68000, SDS-PAGE
270000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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4 * 68000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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enzymatic method of L-tryptophan production. The enzyme is useful for the manufacturing process because it is not inhibited by high levels of product