Information on EC 3.5.1.56 - N,N-dimethylformamidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.56
-
RECOMMENDED NAME
GeneOntology No.
N,N-dimethylformamidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N,N-dimethylformamide + H2O = dimethylamine + formate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glyoxylate and dicarboxylate metabolism
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SYSTEMATIC NAME
IUBMB Comments
N,N-dimethylformamide amidohydrolase
An iron protein. Also acts on N-ethylformamide and N-methyl-formamide and, more slowly, on N,N-diethylformamide, N,N-dimethylacetamide and unsubstituted acyl amides.
CAS REGISTRY NUMBER
COMMENTARY hide
104645-73-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KUFA-1
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-
Manually annotated by BRENDA team
strain KUFA-1
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-
Manually annotated by BRENDA team
DGVK1; strain DGVK1 isolated from soil samples collected from coalmine leftovers
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-
Manually annotated by BRENDA team
DGVK1; strain DGVK1 isolated from soil samples collected from coalmine leftovers
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-
Manually annotated by BRENDA team
JCM 7686
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-
Manually annotated by BRENDA team
strain DMF 3/3
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Manually annotated by BRENDA team
strain DMF 3/3
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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plasmid pAMI2 of Paracoccus aminophilus JCM 7686 carries genetic information which is crucial for the methylotrophic growth, using N,N-dimethylformamide as the sole source of carbon and energy. The plasmid carries a three-gene cluster coding for DmfR, a transcription regulator of the LuxR familiy and two subunits, DmfA1 and DmfA2 of N,N-dimethylformamidase, an enzyme of high substrate specificity that catalyzes the first step in the degradation of N,N-dimethylformamide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
formamide + H2O
NH3 + formate
show the reaction diagram
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3.8% of the activity with N,N-dimethylformamide
-
?
N,N-diethylformamide + H2O
diethylamine + formate
show the reaction diagram
N,N-dimethylacetamide + H2O
dimethylamine + acetate
show the reaction diagram
N,N-dimethylformamide + H2O
dimethylamine + formate
show the reaction diagram
N-ethylformamide + H2O
ethylamine + formate
show the reaction diagram
N-methylformamide + H2O
methylamine + formate
show the reaction diagram
prolinamide + H2O
NH3 + proline
show the reaction diagram
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1.6% of the activity with N,N-dimethylformamide
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N,N-dimethylformamide + H2O
dimethylamine + formate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe
-
iron protein
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
8-hydroxyquinoline
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Mg2+
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64% inhibition, 1 mM
Mn2+
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50% inhibition, 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.28 - 13.8
N,N-Dimethylformamide
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
Mn2+
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.481
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DMFase in cell-free extract of strain DGVK1
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
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assay at
7.5
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optimal pH for temperatures of 45°C and 50°C
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 9.5
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pH 3: 72% of activity maximum, pH 9.5: 73% of activity maximum
4 - 11
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 60
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20 - 80
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20°C: 20% of activity maximum, 80°C: 10% of activity maximum
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15000
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alpha2beta2, 2 * 15000 + 2 * 105000, SDS-PAGE
16000
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alpha2beta2, 2 * 16000 + 2 * 74000
74000
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alpha2beta2, 2 * 16000 + 2 * 74000
105000
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alpha2beta2, 2 * 15000 + 2 * 105000, SDS-PAGE
180000
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gel filtration
250000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
additional information
-
plasmid pAMI2 of Paracoccus aminophilus JCM 7686 carries genetic information which is crucial for the methylotrophic growth, using N,N-dimethylformamide as the sole source of carbon and energy. The plasmid carries a three-gene cluster coding for DmfR, a transcription regulator of the LuxR familiy and two subunits, DmfA1 and DmfA2 of N,N-dimethylformamidase, an enzyme of high substrate specificity that catalyzes the first step in the degradation of N,N-dimethylformamide
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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optimal stability below
50
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30 min, stable
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression in Escherichai coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
environmental protection