Information on EC 3.5.1.51 - 4-acetamidobutyryl-CoA deacetylase

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The expected taxonomic range for this enzyme is: Pseudomonas sp.

EC NUMBER
COMMENTARY hide
3.5.1.51
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RECOMMENDED NAME
GeneOntology No.
4-acetamidobutyryl-CoA deacetylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-acetamidobutanoyl-CoA + H2O = acetate + 4-aminobutanoyl-CoA
show the reaction diagram
The enzyme also hydrolyses 4-aminobutanoyl-CoA to aminobutanoate and coenzyme A
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
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SYSTEMATIC NAME
IUBMB Comments
4-acetamidobutanoyl-CoA amidohydrolase
The enzyme also hydrolyses 4-aminobutanoyl-CoA to aminobutanoate and coenzyme A.
CAS REGISTRY NUMBER
COMMENTARY hide
79121-96-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain B4
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-acetamidoacetyl-CoA + H2O
acetate + aminoacetyl-CoA
show the reaction diagram
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-
-
?
3-acetamidopropionyl-CoA + H2O
acetate + 3-aminopropionyl-CoA
show the reaction diagram
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-
-
?
4-acetamidobutanoyl-CoA + H2O
acetate + 4-aminobutanoyl-CoA
show the reaction diagram
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-
-
ir
4-acetamidobutyryl pantetheine + H2O
acetate + 4-aminobutyrylpantetheine
show the reaction diagram
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-
-
?
4-aminobutanoyl-CoA + H2O
4-aminobutanoate + coenzyme A
show the reaction diagram
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-
-
?
4-butyramidobutyryl-CoA + H2O
butanoate + 4-aminobutyryl-CoA
show the reaction diagram
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-
-
?
4-propionamidobutyryl-CoA + H2O
propionate + 4-aminobutyryl-CoA
show the reaction diagram
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-
-
?
5-acetamidovaleryl-CoA + H2O
acetate + aminovaleryl-CoA
show the reaction diagram
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-
-
?
acetoacetyl-CoA + H2O
?
show the reaction diagram
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-
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?
acetyl-CoA + H2O
?
show the reaction diagram
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very poor substrate
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-
?
beta-alanyl-CoA + H2O
beta alanine + CoA
show the reaction diagram
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-
-
-
?
butyryl-CoA + H2O
butanoate + CoA
show the reaction diagram
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-
-
-
?
DL-3-aminobutyryl-CoA + H2O
DL-3-aminobutyrate + CoA
show the reaction diagram
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-
-
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?
propionyl-CoA + H2O
?
show the reaction diagram
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-
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?
valeryl-CoA + H2O
valerate + CoA
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-acetamidobutanoyl-CoA + H2O
acetate + 4-aminobutanoyl-CoA
show the reaction diagram
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-
-
ir
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
-
10% inhibition at 0.5 mM
EDTA
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26% inhibition at 62 mM
ZnCl2
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30% inhibition at 0.5 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.077
2-Acetamidoacetyl-CoA
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0.048
3-acetamidopropionyl-CoA
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1.5
4-acetamidobutyryl-pantetheine
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0.02
4-Butyramidobutyryl-CoA
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-
0.05
4-Propionamidobutyryl-CoA
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0.005
5-acetamidovaleryl CoA
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1
acetoacetyl-CoA
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0.059
beta-alanyl-CoA
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0.007 - 0.02
butyryl-CoA
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estimated from rates at lower and higher substrate concentration
0.016
DL-3-aminobutyryl-CoA
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0.005 - 0.012
propionyl-CoA
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estimated from rates at lower and higher substrate concentration
0.007 - 0.026
valeryl-CoA
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estimated from rates at lower and higher substrate concentration
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6 - 8.3
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36500
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8 * 36500, SDS-PAGE
275000
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
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8 * 36500, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10 mM potassium phosphate, pH 7, 40 mM KCl or -75°C, 2 years, 50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
over 50fold
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