Information on EC 3.5.1.43 - peptidyl-glutaminase

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The expected taxonomic range for this enzyme is: Bacillus circulans

EC NUMBER
COMMENTARY hide
3.5.1.43
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RECOMMENDED NAME
GeneOntology No.
peptidyl-glutaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-N-peptidyl-L-glutamine + H2O = alpha-N-peptidyl-L-glutamate + NH3
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of amide bond
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-
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SYSTEMATIC NAME
IUBMB Comments
peptidyl-L-glutamine amidohydrolase
Specific for the hydrolysis of the gamma-amide of glutamine substituted at the alpha-amino group, e.g., glycyl-L-glutamine, N-acetyl-L-glutamine and L-leucylglycyl-L-glutamine.
CAS REGISTRY NUMBER
COMMENTARY hide
37228-70-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzyloxycarbonyl-D-Gln + H2O
benzyloxycarbonyl-D-Glu + NH3
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Gln + H2O
benzyloxycarbonyl-L-Glu + NH3
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Gln-Gly + H2O
benzyloxycarbonyl-L-Glu-Gly + NH3
show the reaction diagram
-
peptidoglutaminase II
-
-
?
benzyloxycarbonyl-L-Gln-Gly methyl ester + H2O
benzyloxycarbonyl-L-Glu-Gly methyl ester + NH3
show the reaction diagram
-
peptidoglutaminase II
-
-
?
benzyloxycarbonyl-L-Gln-L-Pro + H2O
benzyloxycarbonyl-L-Glu-L-Pro + NH3
show the reaction diagram
-
peptidoglutaminase II
-
-
?
Gly-L-Gln + H2O
Gly-L-Glu + NH3
show the reaction diagram
-
-
-
-
?
Gly-L-Pro-L-Leu-Gly-L-Gln + H2O
Gly-L-Pro-L-Leu-Gly-L-Glu + NH3
show the reaction diagram
L-Gln + H2O
L-Glu + NH3
show the reaction diagram
-
-
-
-
?
L-Gln-Gly + H2O + H2O
L-Glu-Gly + NH3
show the reaction diagram
L-His-L-Ser-L-Gln-Gly-L-Thr-L-Phe-L-Thr + H2O
L-His-L-Ser-L-Glu-Gly-L-Thr-L-Phe-L-Thr + NH3
show the reaction diagram
-
-
-
-
?
L-Ile-L-Gln-L-Asn-L-CysH-L-Pro-L-Leu-Gly-acetate + H2O
L-Ile-L-Glu-L-Asn-L-CysH-L-Pro-L-Leu-Gly-acetate + NH3
show the reaction diagram
-
-
-
-
?
L-Leu-Gly-L-Gln + H2O
L-Leu-Gly-L-Glu + NH3
show the reaction diagram
-
-
-
-
?
L-Lys-L-Gln-Gly + H2O
L-Lys-L-Glu-Gly + NH3
show the reaction diagram
-
-
-
-
?
L-Phe-L-Gln-Gly + H2O
L-Phe-L-Glu-Gly + NH3
show the reaction diagram
-
-
-
-
?
L-Pro-L-Gln + H2O
L-Pro-L-Glu + NH3
show the reaction diagram
-
-
-
-
?
L-Pro-L-Leu-Gly-L-Gln + H2O
L-Pro-L-Leu-Gly-L-Glu + NH3
show the reaction diagram
L-Ser-L-Gln-Gly + H2O
L-Ser-L-Glu-Gly + NH3
show the reaction diagram
-
-
-
-
?
L-Tyr-L-Gln + H2O
L-Tyr-L-Glu + NH3
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Gln + H2O
N-acetyl-L-Glu + NH3
show the reaction diagram
-
-
-
-
?
phthaloyl-DL-Gln + H2O
?
show the reaction diagram
-
-
-
-
?
tert-amyloxycarbonyl-L-Gln-L-Leu-Gly + H2O
tert-amyloxycarbonyl-L-Glu-L-Leu-Gly + NH3
show the reaction diagram
-
-
-
-
?
tert-amyloxycarbonyl-L-Gln-Pro + H2O
tert-amyloxycarbonyl-L-Glu-L-Leu-Gly + NH3
show the reaction diagram
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peptidoglutaminase II
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-
?
additional information
?
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enzyme catalyzes the hydrolysis of gamma-amide of glutamine whose alpha-amino and/or alpha-carboxyl group participates in peptide bond formation
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
enzyme catalyzes the hydrolysis of gamma-amide of glutamine whose alpha-amino and/or alpha-carboxyl group participates in peptide bond formation
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-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-bromosuccinimide
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sodium lauryl sulfate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.57
benzyloxycarbonyl-D-Gln
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0.11
benzyloxycarbonyl-L-Gln
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0.21
benzyloxycarbonyl-L-Gln-L-Pro
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-
0.33
Gly-L-Gln
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-
0.19
L-Pro-L-Gln
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-
0.16
L-Tyr-L-Gln
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0.35 - 23.3
Leu-Gly-L-Gln
0.1
N-acetyl-L-Gln
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0.12
tert-amyloxycarbonyl-L-Gln-L-Pro
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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hydrolysis of benzyloxycarbonyl-L-Gln
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
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hydrolysis of benzyloxycarbonyl-L-Gln
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
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2 * 42000, peptidoglutaminase I, SDS-PAGE
89000
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equilibrium sedimentation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 42000, peptidoglutaminase I, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no modification
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no carbohydrate detected
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 12.5
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1°C, 22 h, stable; 40°C, 1 h, stable
288897
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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10 min, stable up to
55
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10 min, complete inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE