Information on EC 3.5.1.41 - chitin deacetylase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.5.1.41
-
RECOMMENDED NAME
GeneOntology No.
chitin deacetylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
chitin + H2O = chitosan + acetate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
N-deacetylation
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
-
-
chitin degradation to ethanol
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
chitin amidohydrolase
Hydrolyses the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin.
CAS REGISTRY NUMBER
COMMENTARY hide
56379-60-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Absidia orchidis vel coerulea
-
-
-
Manually annotated by BRENDA team
strain IM-80, ATCC 24905
-
-
Manually annotated by BRENDA team
strain isolated from feces
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
low enzyme level
-
-
Manually annotated by BRENDA team
low enzyme level
-
-
Manually annotated by BRENDA team
low enzyme level
-
-
Manually annotated by BRENDA team
low enzyme level
-
-
Manually annotated by BRENDA team
low enzyme level
-
-
Manually annotated by BRENDA team
AHU 1031; IAM 1069; low enzyme level
-
-
Manually annotated by BRENDA team
AHU 1031
-
-
Manually annotated by BRENDA team
IAM 1069
-
-
Manually annotated by BRENDA team
gene BmCDA7
UniProt
Manually annotated by BRENDA team
strain IFM 46114
-
-
Manually annotated by BRENDA team
strain IFM 46114
-
-
Manually annotated by BRENDA team
strain URM 2136
-
-
Manually annotated by BRENDA team
strain URM 2136
-
-
Manually annotated by BRENDA team
strain IFM 40505
-
-
Manually annotated by BRENDA team
strain IFM 40505
-
-
Manually annotated by BRENDA team
Cunninghamella ramosa
strain URM 1918
-
-
Manually annotated by BRENDA team
Cunninghamella ramosa URM 1918
strain URM 1918
-
-
Manually annotated by BRENDA team
two isozymes
-
-
Manually annotated by BRENDA team
isozymes CDA1 and CDA2
-
-
Manually annotated by BRENDA team
low enzyme level
-
-
Manually annotated by BRENDA team
low enzyme level
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
low enzyme level
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
enzyme silencing results in abnormal molting phenotypes; enzyme silencing results in abnormal molting phenotypes
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(N-acetyl-D-glucosamine)7 + H2O
(GlcN)6GlcNAc + acetate
show the reaction diagram
-
-
-
-
?
2 N,N',N'',N''',N''''-pentaacetylchitopentaose + 3 H2O
GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc + GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + 3 acetate
show the reaction diagram
-
-
initial product: mixture of two isomers, no processivity
?
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-bromoacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNbeta(1-4)GlcNAc + bromoacetate
show the reaction diagram
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-chloroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNbeta(1-4)GlcNAc + chloroacetate
show the reaction diagram
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-fluoroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNbeta(1-4)GlcNAc + fluoroacetate
show the reaction diagram
-
-
-
?
3 N,N',N''-triacetylchitotriose + 2 H2O
GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN + GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc + GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + 3 acetate
show the reaction diagram
-
no detectable hydrolysis at low substrate concentrations i.e. 0.1 mM
initial product: mixture of three isomers
?
4-nitroacetanilide + H2O
?
show the reaction diagram
carboxymethylchitin + H2O
?
show the reaction diagram
-
-
-
-
?
chitin + H2O
?
show the reaction diagram
-
enzyme exhibits high activity on fungal chitosan (78%), shrimp chitosan (83%), partially deacetylated chitin (77%), and chitohexaose (120%)
-
-
?
chitin + H2O
chitosan + acetate
show the reaction diagram
chitin oligomer + H2O
?
show the reaction diagram
chitin-50 + H2O
?
show the reaction diagram
chitobiose + acetate
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-amino-2-deoxy-D-glucose + H2O
show the reaction diagram
-
-
-
?
chitooligosaccharides + H2O
?
show the reaction diagram
-
active on chitooligosaccharides with at least two N-acetyl-glucosamine residues, but the activity increased with the number of N-acetyl-glucosamine residues
-
-
?
chitosan tetramer + acetate
beta-N-acetyl-D-glucosamine-(1-4)-beta-N-acetyl-D-glucosamine-(1-4)-beta-N-acetyl-D-glucosamine-(1-4)-D-glucosamine + H2O
show the reaction diagram
-
-
beta-D-GlcNAc-(1-4)-beta-D-GlcNAc-(1-4)-beta-D-GlcNAc-(1-4)-D-GlcN
?
chitotetraose + acetate
? + H2O
show the reaction diagram
-
-
mixture of partially acetylated compounds
?
CM chitin + H2O
?
show the reaction diagram
-
8% degree of deacetylation with 13% activity compared to water-soluble chitin with degree of deacetylation 50%
-
-
?
crab chitosan + H2O
?
show the reaction diagram
-
71-88% degree of deacetylation with 21-10% activity compared to water-soluble chitin with degree of deacetylation 50%
-
-
?
crab crystalline chitin + H2O
?
show the reaction diagram
-
worst substrate (0.5% deacetylation)
-
-
?
crab swollen chitin + H2O
?
show the reaction diagram
-
1.7% deacetylation
-
-
?
di-N-acetylchitobiose + H2O
?
show the reaction diagram
ethylene glycol chitin + H2O
chitosan + acetate
show the reaction diagram
-
best substrate (24.5% deacetlyation)
-
-
?
GlcNAc-beta-(1-4)-GlcNAc + H2O
acetate + ?
show the reaction diagram
-
20% of the Vmax obtained with glycol-chitin
-
-
?
GlcNAc-beta-(1-4)-GlcNAc + H2O
GlcN-beta-(1-4)-GlcN + 2 acetate
show the reaction diagram
-
-
-
-
?
GlcNAc-beta-(1-4)-GlcNAc + H2O
GlcN-beta-(1-4)-GlcNAc + acetate
show the reaction diagram
-
-
-
-
?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + H2O
GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + GlcN-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + acetate
show the reaction diagram
-
-
-
-
?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
acetate + ?
show the reaction diagram
-
27% of the Vmax obtained with glycol-chitin
-
-
?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
acetate + GlcN-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc + GlcNAc-beta-(1,4)-GlcN-beta-(1,4)-GlcNAc + GlcNAc-beta-(1,4)-GlcNAc-beta-(1,4)-GlcN
show the reaction diagram
-
time courses for hydrolysis of the N-acetyl groups are followed spectrometrically
GlcN-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc, GlcNAc-beta-(1,4)-GlcN-beta-(1,4)-GlcNAc and GlcNAc-beta-(1,4)-GlcNAc-beta-(1,4)-GlcN are formed in the proportions 22:50:28
-
?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN + 3 acetate
show the reaction diagram
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
acetate + ?
show the reaction diagram
-
50% of the Vmax obtained with glycol-chitin
-
-
?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN + 4 acetate
show the reaction diagram
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + acetate
show the reaction diagram
-
time courses for hydrolysis of the N-acetyl groups are followed spectrometrically
-
-
?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
acetate + ?
show the reaction diagram
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN + 5 acetate
show the reaction diagram
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
acetate + ?
show the reaction diagram
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN + 6 acetate
show the reaction diagram
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O
GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcN + 7 acetate
show the reaction diagram
GlcNAc-beta-1,4-GlcNAc + H2O
?
show the reaction diagram
-
-
-
-
?
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O
?
show the reaction diagram
-
-
-
-
?
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O
?
show the reaction diagram
-
-
-
-
?
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O
?
show the reaction diagram
-
-
-
-
?
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O
?
show the reaction diagram
-
-
-
-
?
GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNbeta(1-4)GlcNAc + acetate
show the reaction diagram
-
-
-
?
GlcNAcGlcNAcGlcNAcGlcN + H2O
?
show the reaction diagram
-
-
-
-
?
GlcNAcGlcNGlcNAcGlcNAc + H2O
?
show the reaction diagram
-
-
-
-
?
GlcNGlcNAcGlcNAcGlcNAc + H2O
?
show the reaction diagram
-
-
-
-
?
glycol chitin + H2O
chitosan + acetate
show the reaction diagram
glycol chitosan + H2O
?
show the reaction diagram
-
-
-
-
?
glycol-chitin + H2O
? + acetate
show the reaction diagram
-
-
-
-
?
hepta-N-acetylchitoheptaose + H2O
?
show the reaction diagram
-
100% activity
-
-
?
hexa-N-acetyl-chitohexaose + H2O
chitohexaose + acetate
show the reaction diagram
hexa-N-acetylchitohexaose + H2O
?
show the reaction diagram
hexa-N-acetylchitohexaose + H2O
chitohexaose + acetate
show the reaction diagram
-
-
-
-
?
monoacetylated chitopentaose + H2O
chitopentaose + acetate
show the reaction diagram
-
-
-
-
?
monoacetylated chitotetraose + H2O
chitotetraose + acetate
show the reaction diagram
-
-
-
-
?
monoacetylated chitotriose + H2O
chitotriose + acetate
show the reaction diagram
-
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + 4 H2O
3 GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc + GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + 4 acetate
show the reaction diagram
-
-
initial product: mixture of three isomers, no processivity
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
?
show the reaction diagram
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN + acetate
show the reaction diagram
-
-
end product, initial products: (GlcN)3(GlcN)2GlcNAc + (GlcN)4GlcNAcGlcNAc, processive, reducing-end residue remains intact
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + 5 H2O
chitopentaose + 5 acetate
show the reaction diagram
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
?
show the reaction diagram
N,N',N'',N'''-tetraacetylchitotetraose + 4 H2O
chitotetraose + 4 acetate
show the reaction diagram
N,N',N'',N'''-tetraacetylchitotetraose + H2O
?
show the reaction diagram
N,N',N'',N'''-tetraacetylchitotetraose + H2O
GlcNAc-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc + acetate
show the reaction diagram
-
-
after 10 min, only product, no processivity
?
N,N',N''-triacetylchitotriose + 2 H2O
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc + 2 acetate
show the reaction diagram
N,N',N''-triacetylchitotriose + H2O
?
show the reaction diagram
N,N'-diacetylchitobiose + H2O
2 N-acetyl-D-glucosamine
show the reaction diagram
N-acetylglucosamine oligomer + H2O
?
show the reaction diagram
p-nitrophenyl 2-amino-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranoside + acetate
p-nitrophenyl N,N'-diacetyl-beta-chitobioside + H2O
show the reaction diagram
-
-
-
?
penta-N-acetyl-chitopentaose + H2O
chitopentaose + acetate
show the reaction diagram
penta-N-acetylchitopentaose + H2O
?
show the reaction diagram
shrimp alpha-chitin powder + H2O
?
show the reaction diagram
-
0.7% deacetylation
-
-
?
shrimp swollen chitin + H2O
?
show the reaction diagram
-
2.1% deacetylation
-
-
?
squid beta-chitin powder + H2O
?
show the reaction diagram
-
3.6% deacetylation
-
-
?
swollen chitin + H2O
?
show the reaction diagram
-
8% degree of deacetylation with 6% activity compared to water-soluble chitin with degree of deacetylation 50%
-
-
?
tetra-N-acetyl-chitotetraose + H2O
chitotetraose + acetate
show the reaction diagram
tetra-N-acetylchitotetraose + H2O
?
show the reaction diagram
tri-N-acetylchitotriose + H2O
?
show the reaction diagram
WSCT-50 + H2O
? + acetate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
chitin + H2O
chitosan + acetate
show the reaction diagram
glycol chitin + H2O
chitosan + acetate
show the reaction diagram
-
the chitin deacetylase activity from Flammulina velutipes is determined as around 13fold higher on substrate of chitosan than glycol chitin
-
-
r
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
activates
Fe2+
-
activates
K+
-
activates at 1 mM
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
Ba2+
-
77.9% residual activity at 10 mM
Bi2+
-
; inhibitory at 1 mM
citrate
-
40 mM, 44% inhibition
CN-
-
40 mM, 56% inhibition
glycol chitosan
-
-
-
Mo2+
-
; inhibitory at 1 mM
NH4+
-
40 mM, 30% inhibition
Tartrate
-
40 mM, 22% inhibition
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
-
acetate
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.6
(GlcNAc)4
-
pH 8.0, 50C
18.4
(N-acetyl-D-glucosamine)2
-
-
11
(N-acetyl-D-glucosamine)3
-
-
0.0816 - 0.6
(N-acetyl-D-glucosamine)4
0.0405 - 0.414
(N-acetyl-D-glucosamine)5
0.102
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-bromoacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose
-
pH 8.5, 30C, continuous spectrophotometric assay
0.18
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-chloroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose
-
pH 8.5, 30C, continuous spectrophotometric assay
0.14
2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-fluoroacetamido-2-deoxy-beta-D-glucopyranosyl-(1-4)-2-acetamido-2-deoxy-D-glucose
-
pH 8.5, 30C, continuous spectrophotometric assay
0.09 - 18
GlcNAc-beta-(1-4)-GlcNAc
0.15 - 4.3
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc
0.125 - 0.5
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc
0.079 - 2.5
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc
0.048 - 0.12
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc
18
GlcNAcbeta(1-4)GlcNAc
-
pH 8.5, 30C, continuous spectrophotometric assay
0.66 - 4.3
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
0.125
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
pH 8.5, 30C, continuous spectrophotometric assay
0.087
GlcNAcGlcNAcGlcNAcGlcN
-
-
0.0814
GlcNAcGlcNGlcNAcGlcNAc
-
-
0.307
GlcNGlcNAcGlcNAcGlcNAc
-
-
2.55 - 2.6
glycol chitin
-
2.96
glycol-chitin
-
37C
1.5
hexa-N-acetyl-chitohexaose
-
pH 8.0, 50C, recombinant enzyme
1.1
hexa-N-acetylchitohexaose
-
at pH 6.0 and 50C
5.7
penta-N-acetyl-chitopentaose
-
pH 8.0, 50C, recombinant enzyme
8.9
tetra-N-acetyl-chitotetraose
-
pH 8.0, 50C, recombinant enzyme
additional information
additional information
-
Michaelis-Menten kinetics
-