Information on EC 3.5.1.40 - acylagmatine amidase

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The expected taxonomic range for this enzyme is: Fusarium anguioides

EC NUMBER
COMMENTARY hide
3.5.1.40
-
RECOMMENDED NAME
GeneOntology No.
acylagmatine amidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
benzoylagmatine + H2O = benzoate + agmatine
show the reaction diagram
Also acts on acetylagmatine, propanoylagmatine and bleomycin B2
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
benzoylagmatine amidohydrolase
Also acts on acetylagmatine, propanoylagmatine and bleomycin B2
CAS REGISTRY NUMBER
COMMENTARY hide
39419-74-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl agmatine + H2O
acetic acid + agmatine
show the reaction diagram
-
-
-
?
benzoyl agmatine + H2O
benzoic acid + agmatine
show the reaction diagram
-
-
-
?
bleomycin B2 + H2O
bleomycinic acid + agmatine
show the reaction diagram
propionyl agmatine + H2O
propionic acid + agmatine
show the reaction diagram
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
84% inhibition at 50 mM
benzoyl-L-Arg-ethylester
-
Ki: 3.6 mM
benzoyl-L-Arg-NH2
-
Ki: 5 mM
Co2+
-
74% inhibition at 10 mM
Ni2+
-
85% inhibition at 10 mM
p-chloromercuribenzoic acid
-
77% inhibition at 0.1 mM, restored by addition of cysteine or 2-mercaptoethanol
Zn2+
-
95% inhibition at 10 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.33
acetyl agmatine
-
-
0.22
benzoyl agmatine
-
-
0.8
bleomycin B2
-
-
2
propionyl agmatine
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.6
benzoyl-L-Arg-ethylester
-
-
5
benzoyl-L-Arg-NH2
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65000
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE