Information on EC 3.5.1.39 - alkylamidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.39
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RECOMMENDED NAME
GeneOntology No.
alkylamidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-methylhexanamide + H2O = hexanoate + methylamine
show the reaction diagram
The enzyme hydrolyzes N-monosubstituted and N,N-disubstituted amides, and there is some activity towards primary amides. It has little or no activity towards short-chain substrates
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
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-
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SYSTEMATIC NAME
IUBMB Comments
N-methylhexanamide amidohydrolase
The enzyme hydrolyses N-monosubstituted and N,N-disubstituted amides, and there is some activity towards primary amides. It has little or no activity towards short-chain substrates.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-19-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Ovis aries aries
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N,N-dimethylcaproamide + H2O
dimethylamine + hexanoate
show the reaction diagram
Ovis aries aries
-
-
-
?
N-butylcaproamide + H2O
butylamine + hexanoate
show the reaction diagram
Ovis aries aries
-
-
-
?
N-ethylcaproamide + H2O
ethylamine + hexanoate
show the reaction diagram
Ovis aries aries
-
-
-
?
N-methylbutyramide + H2O
methylamine + butyric acid
show the reaction diagram
Ovis aries aries
-
-
-
?
N-methylcaproamide + H2O
methylamine + hexanoate
show the reaction diagram
Ovis aries aries
-
-
-
?
N-methylcaprylamide + H2O
methylamine + octanoate
show the reaction diagram
Ovis aries aries
-
low activity
-
?
N-methylheptylamide + H2O
methylamine + heptanoate
show the reaction diagram
Ovis aries aries
-
-
-
?
N-methylvaleramide + H2O
methylamine + pentanoate
show the reaction diagram
Ovis aries aries
-
unable to hydrolyze lower members of N-methyl substituted amides
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?
N-phenylcaproamide + H2O
phenylamine + hexanoate
show the reaction diagram
Ovis aries aries
-
-
-
?
N-propylcaproamide + H2O
propylamine + hexanoate
show the reaction diagram
Ovis aries aries
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
O,O-diethyl p-nitrophenyl phosphate
Ovis aries aries
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inhibition at 0.001 mM
p-chloromercuribenzoate
Ovis aries aries
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20% inhibition at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19
N,N-dimethylcaproamide
Ovis aries aries
-
inhibition by excess of substrate
2.2
N-Butylcaproamide
Ovis aries aries
-
inhibition by excess of substrate
0.83
N-ethylcaproamide
Ovis aries aries
-
inhibition by excess of substrate
0.66
N-methylcaproamide
Ovis aries aries
-
inhibition by excess of substrate
3.7
N-methylcaprylamide
Ovis aries aries
-
inhibition by excess of substrate
1.25
N-methylheptylamide
Ovis aries aries
-
inhibition by excess of substrate
5
N-methylvaleramide
Ovis aries aries
-
inhibition by excess of substrate
2.5
N-phenylcaproamide
Ovis aries aries
-
inhibition by excess of substrate
1.8
N-propylcaproamide
Ovis aries aries
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inhibition by excess of substrate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.12
Ovis aries aries
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
Ovis aries aries
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-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Ovis aries aries
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Ovis aries aries
-
-
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
230000 - 250000
Ovis aries aries
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gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, 2step chromatography
Ovis aries aries
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