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Information on EC 3.5.1.39 - alkylamidase

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The enzyme appears in viruses and cellular organisms

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3.5.1.39

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alkylamidase

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N-methylhexanamide + H2O = hexanoate + methylamine

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The enzyme hydrolyzes N-monosubstituted and N,N-disubstituted amides, and there is some activity towards primary amides. It has little or no activity towards short-chain substrates

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carboxylic acid amide hydrolysis

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-

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N-methylhexanamide amidohydrolase

The enzyme hydrolyses N-monosubstituted and N,N-disubstituted amides, and there is some activity towards primary amides. It has little or no activity towards short-chain substrates.

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62213-19-8

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Ovis aries aries

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Manually annotated by BRENDA team

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N,N-dimethylcaproamide + H2O

dimethylamine + hexanoate

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Ovis aries aries

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-

?

N-butylcaproamide + H2O

butylamine + hexanoate

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Ovis aries aries

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-

-

?

N-ethylcaproamide + H2O

ethylamine + hexanoate

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Ovis aries aries

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-

-

?

N-methylbutyramide + H2O

methylamine + butyric acid

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Ovis aries aries

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-

-

?

N-methylcaproamide + H2O

methylamine + hexanoate

show the reaction diagram

Ovis aries aries

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-

-

?

N-methylcaprylamide + H2O

methylamine + octanoate

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Ovis aries aries

-

low activity

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?

N-methylheptylamide + H2O

methylamine + heptanoate

show the reaction diagram

Ovis aries aries

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-

-

?

N-methylvaleramide + H2O

methylamine + pentanoate

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Ovis aries aries

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unable to hydrolyze lower members of N-methyl substituted amides

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?

N-phenylcaproamide + H2O

phenylamine + hexanoate

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Ovis aries aries

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-

-

?

N-propylcaproamide + H2O

propylamine + hexanoate

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Ovis aries aries

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-

-

?

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O,O-diethyl p-nitrophenyl phosphate

Ovis aries aries

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inhibition at 0.001 mM

p-chloromercuribenzoate

Ovis aries aries

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20% inhibition at 1 mM

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0.19

N,N-dimethylcaproamide

Ovis aries aries

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inhibition by excess of substrate

2.2

N-Butylcaproamide

Ovis aries aries

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inhibition by excess of substrate

0.83

N-ethylcaproamide

Ovis aries aries

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inhibition by excess of substrate

0.66

N-methylcaproamide

Ovis aries aries

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inhibition by excess of substrate

3.7

N-methylcaprylamide

Ovis aries aries

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inhibition by excess of substrate

1.25

N-methylheptylamide

Ovis aries aries

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inhibition by excess of substrate

5

N-methylvaleramide

Ovis aries aries

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inhibition by excess of substrate

2.5

N-phenylcaproamide

Ovis aries aries

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inhibition by excess of substrate

1.8

N-propylcaproamide

Ovis aries aries

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inhibition by excess of substrate

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0.12

Ovis aries aries

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-

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9

Ovis aries aries

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-

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Ovis aries aries

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Manually annotated by BRENDA team

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Ovis aries aries

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Manually annotated by BRENDA team

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230000 - 250000

Ovis aries aries

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gel filtration

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to homogeneity, 2step chromatography

Ovis aries aries

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Show References (1 entry)

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NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)