Information on EC 3.5.1.33 - N-acetylglucosamine deacetylase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.5.1.33
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RECOMMENDED NAME
GeneOntology No.
N-acetylglucosamine deacetylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-acetyl-D-glucosamine + H2O = D-glucosamine + acetate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of amide bond
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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chitin degradation I (archaea)
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-
SYSTEMATIC NAME
IUBMB Comments
N-acetyl-D-glucosamine amidohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9012-32-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 4157; B; stain K-12
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
non-O1 1148A
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-
Manually annotated by BRENDA team
non-O1 1148A
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-acetyl-D-galactosamine + H2O
galactosamine + acetate
show the reaction diagram
N-acetyl-D-glucosamine + H2O
D-glucosamine + acetate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-acetyl-D-glucosamine + H2O
D-glucosamine + acetate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
120 - 127
N-acetyl-D-glucosamine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.53
N-acetyl-D-glucosamine
Thermococcus kodakarensis
Q6F4N1
pH 8.5, 75°C
300
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 8.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9.5
-
pH 6: 5% of maximal activity, pH 9.5: 10% of maximal activity
37 - 100
activity levels at 37°C and 100°C are about 20% of that observed at the optimal temperature (75°C)
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
6 * 30000, SDS-PAGE
31595
-
6 * 31595, calculated from sequence
31600
-
x * 31600, SDS-PAGE
52700
-
calculation from sequence of DNA
160000
gel filtration
174000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homohexamer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
1°C, 4 h, complete loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression analysis through RT-PCR
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expression in Escherichia coli
expression of a combined glucosaminyl N-deacetylase/N-sulfotransferase in human kidney cell line 293
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overexpressed as inclusion bodies in Escherichia coli Rosetta (DE3) pLys. The insoluble inclusion body is solubilized and reactivated through a refolding procedure
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the transcription is specifically induced by N,N'-diacetylchitobiose, suggesting the function of this gene in chitin catabolism in vivo. N-acetyl-D-glucosamine can not act as an inducer
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
nonfunctional enzyme mutant, hypersensitive to exogenous lysozymem reduced virulence
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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nonfunctional enzyme mutant, reduced virulence in intraperitoneal mouse model