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Information on EC 3.5.1.28 - N-acetylmuramoyl-L-alanine amidase and Organism(s) Drosophila melanogaster and UniProt Accession Q70PY2

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This record set is specific for:
Drosophila melanogaster
UNIPROT: Q70PY2 not found.
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The taxonomic range for the selected organisms is: Drosophila melanogaster
The enzyme appears in selected viruses and cellular organisms
Synonyms
n-acetylmuramoyl-l-alanine amidase, pglyrp2, cell wall hydrolase, t7 lysozyme, n-acetylmuramyl-l-alanine amidase, phage endolysin, namlaa, pgrp-l, cwlj1, peptidoglycan amidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetylmuramoyl-alanine amidase
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acetylmuramyl-alanine amidase
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acetylmuramyl-L-alanine amidase
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Autolysin
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Cell wall hydrolase
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Lytic amidase
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Mucopeptide aminohydrolase
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murein hydrolase
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N-acetylmuramic acid L-alanine amidase
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N-acetylmuramoyl-L-alanine amidase
N-acetylmuramoyl-L-alanine amidase type I
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N-acetylmuramoyl-L-alanine amidase type II
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N-acetylmuramyl-L-alanine amidase
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N-acetylmuramylalanine amidase
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N-acylmuramyl-L-alanine amidase
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ORFL3
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peptidoglycan recognition protein SC1a
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T3 lysozyme
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T7 lysozyme
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additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
peptidoglycan amidohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9013-25-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
show the reaction diagram
peptidoglycan + H2O
?
show the reaction diagram
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enzyme is required for the recognition of peptidoglycan, a component of bacterial cell walls, and therefore critical for the immune response
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?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-acetylmuramoyl-L-alanine + H2O
N-acetylmuramate + L-alanine
show the reaction diagram
the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, the immunostimulatory properties of PGRP-SC1B-degraded peptidoglycan are highly reduced
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peptidoglycan + H2O
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show the reaction diagram
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enzyme is required for the recognition of peptidoglycan, a component of bacterial cell walls, and therefore critical for the immune response
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?
additional information
?
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the enzyme performs cell wall lysis, and shows antibacterial activity against Bacillus megaterium
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
inactivation through depletion of zinc ions
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
the enzyme shows highest activity with peptidoglycan substrates from Staphylococcus aureus, and 34% and 23% of this activity with substrate from Micrococcus luteus and Bacillus megaterium, respectively, at pH 8.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene PGRP-SB1 or CG9681; gene PGRP-SB1 or CG9681
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PGSB1_DROME
190
1
20998
Swiss-Prot
Secretory Pathway (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C168A
site-directed mutagenesis, the mutant is enzymatically inactive but retains its peptidoglycan affinity
C168S
site-directed mutagenesis, the mutant is enzymatically inactive but retains its peptidoglycan affinity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene PGRP-SB1, recombinant His6-tagged wild-type and mutant enzyme by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene PGRP-SB1, DNA and amino acid sequence determination and analysis, expression of His6-tagged 128 C-terminal residues and of his6-tagged 180 N-terminal residues in Escherichia coli strain XL-1I Blue
gene PGRP-SB1, expression of His6-tagged wild-type and mutant enzyme
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mellroth, P.; Steiner, H.
PGRP-SB1: an N-acetylmuramoyl L-alanine amidase with antibacterial activity
Biochem. Biophys. Res. Commun.
350
994-999
2006
Drosophila melanogaster (Q70PY2)
Manually annotated by BRENDA team
Mellroth, P.; Karlsson, J.; Steiner, H.
A scavenger function for a Drosophila peptidoglycan recognition protein
J. Biol. Chem.
278
7059-7064
2003
Drosophila melanogaster (Q70PY2)
Manually annotated by BRENDA team
Garver, L.S.; Wu, J.; Wu, L.P.
The peptidoglycan recognition protein PGRP-SC1a is essential for Toll signaling and phagocytosis of Staphylococcus aureus in Drosophila
Proc. Natl. Acad. Sci. USA
103
660-665
2006
Drosophila melanogaster
Manually annotated by BRENDA team