Information on EC 3.5.1.20 - citrullinase

Word Map on EC 3.5.1.20
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.5.1.20
-
RECOMMENDED NAME
GeneOntology No.
citrullinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-citrulline + H2O = L-ornithine + CO2 + NH3
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-proline biosynthesis II (from arginine)
-
-
proline metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
L-citrulline N5-carbamoyldihydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
59088-17-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
protozoan, strain Z
-
-
Manually annotated by BRENDA team
Euphorbia sp.
pinea, characias, hyssopifolia
-
-
Manually annotated by BRENDA team
strain Schu S4, gene ctu or FTT0435
-
-
Manually annotated by BRENDA team
human
-
-
Manually annotated by BRENDA team
cassava
-
-
Manually annotated by BRENDA team
Phytomonas davidi
trypanosomatide, parasite in euphorbs and asclepiads and other plants
-
-
Manually annotated by BRENDA team
tomato
-
-
Manually annotated by BRENDA team
protozoan; thermophila, pyryformis
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
citrulline + H2O
ornithine + CO2 + NH3
show the reaction diagram
L-citrulline + H2O
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-citrulline + H2O
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
HgCl2
-
1 mM, inhibition reversed by 2-mercaptoethanol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.06 - 4.55
citrulline
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.2
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 37
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 60
-
stable up to 40C for 10 min, retains 87% activity after treatment at 50C, activity completely lost at 60C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
-
3 * 31000, 3 identical subunits, SDS-PAGE
94000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
-
3 * 31000, 3 identical subunits, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 60
-
stable up to 40C for 10 min, retains 87% activity after treatment at 50C, activity completely lost at 60C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10C stable for several days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
generation of a nonpolar deletion DELTActu mutant. Deletion of the ctu gene results in loss of CTU activity, which is restored by transcomplementing the ctu gene. Loss of CTU does not affect acellular growth, but attenuates intramacrophage survival. Mice infected intranasally with the DELTActu mutant show significantly reduced bacterial burden in the lungs, liver, and spleen compared to wild-type Schu S4-infected mice.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
clinical biochemistry, therapeutic measurement of citrulline