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Information on EC 3.5.1.2 - glutaminase and Organism(s) Bacillus subtilis and UniProt Accession O07637

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.2 glutaminase
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This record set is specific for:
Bacillus subtilis
UNIPROT: O07637 not found.
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The taxonomic range for the selected organisms is: Bacillus subtilis
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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L-glutamine
+
H2O
=
L-glutamate
+
NH3
+
=
+
Synonyms
glutaminase, l-glutaminase, phosphate-activated glutaminase, mitochondrial glutaminase, glutaminase 1, kidney-type glutaminase, phosphate activated glutaminase, glutaminase a, glnase, glutaminase c, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GLS
-
-
-
-
glutaminase I
-
-
-
-
glutamine aminohydrolase
-
-
-
-
K-glutaminase
-
-
-
-
L-glutaminase
-
-
-
-
L-glutamine amidohydrolase
-
-
-
-
PAG
-
-
-
-
phosphate activated glutaminase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-glutamine amidohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9001-47-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-glutamate-p-nitroanilide + H2O
L-glutamate + 4-nitroaniline
show the reaction diagram
-
-
-
?
L-glutamine + H2O
L-glutamic acid + NH3
show the reaction diagram
-
-
-
?
L-glutamate-p-nitroanilide + H2O
L-glutamate + 4-nitroaniline
show the reaction diagram
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
show the reaction diagram
-
biosynthesis of pyridoxal-5’-phosphate
-
-
?
L-glutamine + H2O
L-glutamic acid + NH3
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-glutamine + H2O
L-glutamate + NH3
show the reaction diagram
-
biosynthesis of pyridoxal-5’-phosphate
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6-diazo-5-oxo-L-norleucine
-
6-diazo-5-oxo-L-norleucine
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
27.6
L-glutamine
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
38.6
L-glutamine
-
67.7
L-glutamine
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.7 - 5.5
Mg2+
Bacillus subtilis
-
0.7 - 1.3
Mn2+
Bacillus subtilis
-
1.7 - 5.5
Mg2+
Bacillus subtilis
-
0.7 - 1.3
Mn2+
Bacillus subtilis
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 23857D-5
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
2 * 34000, SDS-PAGE
72600
gel filtration
138100
gel filtration
36200
4 * 36200, SDS-PAGE, gelfiltration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 34000, SDS-PAGE
homotetramer
4 * 36200, SDS-PAGE, gelfiltration
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no modification
-
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
handing-drop vapor diffusion technique, at 18ºC
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by centrifugation, lysis, nickel affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bauer, J.A.; Bennett, E.M.; Begley, T.P.; Ealick, S.E.
Three-dimensional structure of YaaE from Bacillus subtilis, a glutaminase implicated in pyridoxal-5'-phosphate biosynthesis
J. Biol. Chem.
279
2704-2711
2004
Bacillus subtilis
Manually annotated by BRENDA team
Brown, G.; Singer, A.; Proudfoot, M.; Skarina, T.; Kim, Y.; Chang, C.; Dementieva, I.; Kuznetsova, E.; Gonzalez, C.F.; Joachimiak, A.; Savchenko, A.; Yakunin, A.F.
Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis
Biochemistry
47
5724-5735
2008
Bacillus subtilis (O07637), Bacillus subtilis (O31465), Bacillus subtilis, Escherichia coli (P0A6W0), Escherichia coli (P77454), Escherichia coli, Bacillus subtilis 168 (O07637), Bacillus subtilis 168 (O31465), Escherichia coli W3110 / ATCC 27325 (P0A6W0), Escherichia coli W3110 / ATCC 27325 (P77454)
Manually annotated by BRENDA team