Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.5.1.15 - aspartoacylase and Organism(s) Rattus norvegicus and UniProt Accession Q9R1T5

for references in articles please use BRENDA:EC3.5.1.15
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.15 aspartoacylase
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Rattus norvegicus
UNIPROT: Q9R1T5 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
aspartoacylase, human aspa, haspa, aspartoacylase ii, murine aspartoacylase, aminoacylase 2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetyl-aspartic deaminase
-
-
-
-
Acylase II
-
-
-
-
aminoacylase 2
-
-
Aminoacylase II
-
-
-
-
aminoacylase-2
-
-
Aspartoacylase
-
-
N-acetyl-aspartate deacetylase
-
-
N-Acetyl-L-aspartate amidohydrolase
-
-
-
-
N-Acetylaspartate amidohydrolase
-
-
-
-
NAA acylase
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of amide bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
N-acyl-L-aspartate amidohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9031-86-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-acetylaspartic acid + H2O
L-asparatate + acetate
show the reaction diagram
acetyl-DL-alanine + H2O
acetate + DL-alanine
show the reaction diagram
-
-
-
-
?
acetyl-DL-methionine + H2O
acetate + methionine
show the reaction diagram
-
-
-
-
?
acetyl-L-glutamate + H2O
acetate + glutamate
show the reaction diagram
-
-
-
-
?
acetyl-L-leucine + H2O
acetate + leucine
show the reaction diagram
-
-
-
-
?
chloroacetyl-DL-alanine + H2O
chloroacetate + DL-alanine
show the reaction diagram
-
-
-
-
?
chloroacetyl-DL-norleucine + H2O
chloroacetate + DL-norleucine
show the reaction diagram
-
-
-
-
?
chloroacetyl-L-aspartate + H2O
chloroacetate + L-aspartate
show the reaction diagram
-
-
-
-
?
chloroacetyl-L-leucine + H2O
chloroacetate + L-leucine
show the reaction diagram
-
-
-
-
?
N-acetyl-L-aspartate + H2O
aspartate + acetate
show the reaction diagram
-
deficiency in enzyme activity leads to spongiform degeneration of the white matter of the brain and is the established cause of Canavan disease
-
-
?
N-acetyl-L-aspartate + H2O
L-aspartate + acetic acid
show the reaction diagram
-
-
-
-
?
N-acetyl-L-aspartic acid + H2O
acetate + aspartic acid
show the reaction diagram
-
-
-
-
?
N-acetylaspartic acid + H2O
aspartate + acetate
show the reaction diagram
N-acetylaspartic acid + H2O
L-asparatate + acetate
show the reaction diagram
N-acyl L-aspartic acid + H2O
acetate + L-aspartate
show the reaction diagram
-
-
-
-
?
N-formyl aspartic acid + H2O
formate + aspartate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
N-methyl aspartic acid, N-carbamoyl aspartic acid and N-glycyl aspartic acids are no substrates
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-acetylaspartic acid + H2O
L-asparatate + acetate
show the reaction diagram
the enzyme is involved in negative regulation of brain-derived neurotrophic factor, BDNF, and timing of postnatal oligodendrogenesis, overview
-
-
?
N-acetyl-L-aspartate + H2O
aspartate + acetate
show the reaction diagram
-
deficiency in enzyme activity leads to spongiform degeneration of the white matter of the brain and is the established cause of Canavan disease
-
-
?
N-acetyl-L-aspartic acid + H2O
acetate + aspartic acid
show the reaction diagram
-
-
-
-
?
N-acetylaspartic acid + H2O
aspartate + acetate
show the reaction diagram
N-acetylaspartic acid + H2O
L-asparatate + acetate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
necessary for activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aspartic acid
-
0.002 M, 20-25% inhibition
glutamic acid
-
0.002 M, 20-25% inhibition
N-ethylmaleimide
-
-
p-hydroxymercuribenzoate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
chloroacetylamino acids
-
-
-
Triton X-100
-
increases activity of the enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
N-acetyl-L-aspartic acid
-
-
0.2
N-acyl L-aspartic acid
-
brain enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
-
-
8
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
female wild-type Kyoto-Wistar and tremor rats
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
in situ hybridization, quantitative expression analysis in brain of wild-type and tremor rats, overview
Manually annotated by BRENDA team
primary cortical neuron culture
Manually annotated by BRENDA team
neurons from wild-type embryos are co-cultured with oligodendrocytes prepared from either wild-type or tremor rats
Manually annotated by BRENDA team
primary cell culture
Manually annotated by BRENDA team
-
immunohistochemical localization analysis, high enzyme content in oligodendrocytes along with CC1, and in white matter, including the corpus callosum and the cerebellar white matter, less abundant enzyme content in grey matter, including cerebral cortex, descending neuronal fibers, and microglial cells, no enzyme in astroyctes and forebrain, overview
Manually annotated by BRENDA team
additional information
-
immunocolorimetric analysis of enzyme distribution in the brain, enzyme staining does not colocalize with CC1
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
enzyme deficiency is involved in Canavan disease and type 2 diabetes, high levels of N-acetylaspartic acid induce inflammatory agents TNFalpha, p38MAPK, iNOS, PKC, COX2 and ICAM3, and alters proteins levels and smooth muscle contractility, which contributes to the gastrointestinal disorder, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ACY2_RAT
312
0
35314
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
-
about, gel filtration
38000
-
1 * 38000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 38000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
resolution 1.8 angstrom
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
restoration of deficient enzyme activity in CNS neurons does not ameliorate motor deficits and demyelination in a model of Canavan disease, which is caused by elevated levels of N-acetylaspartic acid, NAA, neuronal expression of ASPA can compensate for NAA-mediated neuronal hyperexcitation, but not for oligodebdrocyte dysfunciton, phenotypes, overview
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57
-
rapidly denatures above
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
pure enzyme extremely unstable
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, frozen storage of undialyzed enzyme for a few days leads to a complete disappearance of activity following a single thaw
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from brain by subcellular fractionation, and anion exchange chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
quantitative expression analysis in brain of wild-type and tremor rats
expression in Escherichia coli
-
expression of the GFP-tagged enzyme in COS-7 cells in cytoplasm, nucleoplasm, and nuclei
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
D'Adamo, A.F.; Wertman, E.; Foster, F.; Schneider, H.
A radiochemical assay for N-acetyl-L-aspartate amidohydrolase (EC 3.5.1.15) and its occurrence in the tissues of the chicken
Life Sci.
23
791-796
1978
Columba sp., Gallus sp., no activity in Cavia porcellus, Rattus norvegicus
Manually annotated by BRENDA team
Endo, Y.
Deacetylation and deformylation of N-acyl amino acids by kidney acylases
FEBS Lett.
95
281-283
1978
Cavia porcellus, Rattus norvegicus, Rattus norvegicus Wistar
Manually annotated by BRENDA team
D'Adamo, A.F.; Peisach, J.; Manner, G.; Weiler, C.T.
N-Acetyl-aspartate amidohydrolase: Purification and properties
J. Neurochem.
28
739-744
1977
Gallus sp., Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Goldstein, F.B.
Amidohydrolases of brain; enzymatic hydrolysis of N-acetyl-L-aspartate and other N-acyl-L-amino acids
J. Neurochem.
26
45-49
1976
Bos taurus, Columba sp., Felis catus, Macaca iris, Macaca mulatta, Mesocricetus auratus, Mus musculus, no activity in Cavia porcellus, Oryctolagus cuniculus, Rattus norvegicus, Rattus norvegicus Sprague Dawley, Sus scrofa
Manually annotated by BRENDA team
D'Adamo, A.F.; Smith, J.C.; Weiler, C.
The occurrence of N-acetylaspartate amidohydrolase (aminoacylase II) in the developing rat
J. Neurochem.
20
1275-1278
1973
Rattus norvegicus, Rattus norvegicus Sprague Dawley, Sus scrofa
Manually annotated by BRENDA team
Baslow, M.H.; Suckow, R.F.; Sapirstein, V.; Hungund, B.L.
Expression of aspartoacylase activity in cultured rat macroglial cells is limited to oligodendrocytes
J. Mol. Neurosci.
13
47-53
1999
Carassius auratus, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Barash, V.; Flhor, D.; Morag, B.; Boneh, A.; Elpeleg, O.N.; Gilon, C.
A radiometric assay for aspartoacylase activity in human fibroblasts: application for the diagnosis of canavan's disease
Clin. Chim. Acta
201
175-182
1991
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Hershfield, J.R.; Madhavarao, C.N.; Moffett, J.R.; Benjamins, J.A.; Garbern, J.Y.; Namboodiri, A.
Aspartoacylase is a regulated nuclear-cytoplasmic enzyme
FASEB J.
20
2139-2141
2006
Rattus norvegicus
Manually annotated by BRENDA team
Madhavarao, C.N.; Moffett, J.R.; Moore, R.A.; Viola, R.E.; Namboodiri, M.A.; Jacobowitz, D.M.
Immunohistochemical localization of aspartoacylase in the rat central nervous system
J. Comp. Neurol.
472
318-329
2004
Rattus norvegicus
Manually annotated by BRENDA team
Francis, J.S.; Olariu, A.; McPhee, S.W.; Leone, P.
Novel role for aspartoacylase in regulation of BDNF and timing of postnatal oligodendrogenesis
J. Neurosci. Res.
84
151-169
2006
Rattus norvegicus (Q9R1T5)
Manually annotated by BRENDA team
Klugmann, M.; Leichtlein, C.B.; Symes, C.W.; Serikawa, T.; Young, D.; During, M.J.
Restoration of aspartoacylase activity in CNS neurons does not ameliorate motor deficits and demyelination in a model of Canavan disease
Mol. Ther.
11
745-753
2005
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Surendran, S.
Upregulation of N-acetylaspartic acid alters inflammation, transcription and contractile associated protein levels in the stomach and smooth muscle contractility
Mol. Biol. Rep.
345
973-975
2007
Rattus norvegicus
Manually annotated by BRENDA team
Bitto, E.; Bingman, C.A.; Wesenberg, G.E.; McCoy, J.G.; Phillips, G.N.
Structure of aspartoacylase, the brain enzyme impaired in Canavan disease
Proc. Natl. Acad. Sci. USA
104
456-461
2007
Rattus norvegicus, Homo sapiens (P45381), Homo sapiens
Manually annotated by BRENDA team
Hanaya, R.; Kiura, Y.; Kurisu, K.; Sakai, N.; Serikawa, T.; Sasa, M.
N-acetyl-L-aspartate activates hippocampal CA3 neurons in rodent slice preparations
Brain Res. Bull.
75
663-667
2008
Rattus norvegicus
Manually annotated by BRENDA team
Surendran, S.
Upregulation of N-acetylaspartic acid alters inflammation, transcription and contractile associated protein levels in the stomach and smooth muscle contractility
Mol. Biol. Rep.
36
201-206
2009
Rattus norvegicus
Manually annotated by BRENDA team