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N-2,2-dimethylpropionyl-L-methionine + H2O
2,2-dimethyl-propionate + L-methionine
-
-
-
?
N-acetyl-glycine + H2O
acetate + glycine
-
-
-
?
N-acetyl-L-alanine + H2O
acetate + L-alanine
-
-
-
?
N-acetyl-L-asparagine + H2O
acetate + L-asparagine
-
-
-
?
N-acetyl-L-cysteine + H2O
acetate + L-cysteine
-
-
-
?
N-acetyl-L-ethionine + H2O
acetate + L-ethionine
-
-
-
?
N-acetyl-L-glutamate + H2O
acetate + L-glutamate
N-acetyl-L-glutamine + H2O
acetate + L-glutamine
-
-
-
?
N-acetyl-L-histidine + H2O
acetate + L-histidine
-
-
-
?
N-acetyl-L-leucine + H2O
acetate + L-leucine
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
N-acetyl-L-norleucine + H2O
acetate + L-norleucine
-
-
-
?
N-acetyl-L-phenylalanine + H2O
acetate + L-phenylalanine
-
-
-
?
N-acetyl-L-threonine + H2O
acetate + L-threonine
-
-
-
?
N-acetyl-L-valine + H2O
acetate + L-valine
-
-
-
?
N-acyl-L-amino acid + H2O
carboxylate + L-amino acid
-
-
-
?
N-benzoyl-L-glutamate + H2O
benzoate + L-glutamate
-
-
-
?
N-benzoyl-L-methionine + H2O
benzoate + L-methionine
-
-
-
?
N-butanoyl-L-methionine + H2O
butanoate + L-methionine
-
-
-
?
N-cyclohexanoyl-L-methionine + H2O
hexanoate + L-methionine
-
-
-
?
N-cyclopentanoyl-L-methionine + H2O
pentanoate + L-methionine
-
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
-
-
-
?
N-hexanoyl-L-methionine + H2O
hexanoate + L-methionine
-
-
-
?
N-isobutanoyl-L-methionine + H2O
isobutanoate + L-methionine
-
-
-
?
N-isopentanoyl-L-methionine + H2O
isopentanoate + L-methionine
-
-
-
?
N-pentanoyl-L-methionine + H2O
pentanoate + L-methionine
-
-
-
?
N-propionyl-L-methionine + H2O
propionate + L-methionine
-
-
-
?
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid + H2O
S-[(1S,2S)-2-hydroxycyclohexyl]cysteine + acetate
-
-
-
-
?
2-acetylamino-3-((3S,4S)-3-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid + H2O
S-[(3S,4S)-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-4-yl]cysteine + acetate
-
-
-
-
?
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid + H2O
S-[(1R,2R)-1-hydroxy-3-oxo-1,3-diphenylpropan-2-yl]cysteine + acetate
-
-
-
-
?
N-acetyl-1,2-dichlorovinyl-L-cysteine + H2O
acetate + 1,2-dichlorovinyl-L-cysteine
-
a metabolite of a xenobiotic trichloroethylene, substrate of AA3
-
-
?
N-acetyl-DL-methionine + H2O
DL-methionine + acetate
-
-
-
-
?
N-acetyl-L-Cys + H2O
acetate + L-Cys
-
-
-
-
?
N-acetyl-L-Met + H2O
acetate + L-Met
-
-
-
-
?
N-acetyl-L-methionine
L-methionine + acetate
-
-
-
-
?
N-acetyl-L-tyrosine + H2O
acetate + L-tyrosine
-
-
-
-
?
Nalpha-acetylmethionine + H2O
acetate + L-methionine
-
-
-
-
?
N-acetyl-L-glutamate + H2O
acetate + L-glutamate
-
-
-
?
N-acetyl-L-glutamate + H2O
acetate + L-glutamate
-
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
?
N-acetyl-L-methionine + H2O
acetate + L-methionine
-
-
-
-
?
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Amniotic Band Syndrome
A comprehensive clinical and genetic study in 127 patients with ID in Kinshasa, DR Congo.
Carcinogenesis
Aminoacylase 1 (ACY-1) Mediates the Proliferation and Migration of Neuroblastoma Cells in Humans Through the ERK/Transforming Growth Factor ? (TGF-?) Signaling Pathways.
Carcinoma
Cobalt-activated acylase and aminoacylase I in human malignant tumors.
Carcinoma
Differential aminoacylase expression in neuroblastoma.
Carcinoma
Effect of the ACY-1 gene on HER2 and TRAIL expression in rectal carcinoma.
Carcinoma
Genetic relationship between acylpeptide hydrolase and acylase, two hydrolytic enzymes with similar binding but different catalytic specificities.
Carcinoma
Genome-wide analysis identifies a tumor suppressor role for aminoacylase 1 in iron-induced rat renal cell carcinoma.
Carcinoma
Probing the dimeric structure of porcine aminoacylase 1 by mass spectrometric and modeling procedures.
Carcinoma, Hepatocellular
Aminoacylase 3 Is a New Potential Marker and Therapeutic Target in Hepatocellular Carcinoma.
Carcinoma, Hepatocellular
Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma.
Carcinoma, Non-Small-Cell Lung
Isolation of two contigs of overlapping cosmids derived from human chromosomal band 3p21.1 and identification of 5 new 3p21.1 genes.
Carcinoma, Non-Small-Cell Lung
Lack of expression of aminoacylase-1 in small cell lung cancer. Evidence for inactivation of genes encoded by chromosome 3p.
Carcinoma, Renal Cell
Differential aminoacylase expression in neuroblastoma.
Carcinoma, Renal Cell
Genome-wide analysis identifies a tumor suppressor role for aminoacylase 1 in iron-induced rat renal cell carcinoma.
Colorectal Neoplasms
Overexpression of aminoacylase 1 is associated with colorectal cancer progression.
Diabetes Mellitus, Type 2
Deciphering the Plasma Proteome of Type 2 Diabetes.
Down Syndrome
A comprehensive clinical and genetic study in 127 patients with ID in Kinshasa, DR Congo.
Dystonia
Expanding the phenotype in aminoacylase 1 (ACY1) deficiency: characterization of the molecular defect in a 63-year-old woman with generalized dystonia.
Hepatitis B
Identification and clinical significance of an elevated level of serum aminoacylase-1 autoantibody in patients with hepatitis B virus-related liver cirrhosis.
Hepatitis C
Aminoacylase 3 binds to and cleaves the N-terminus of the hepatitis C virus core protein.
Herpes Simplex
Integration site(s) of herpes simplex virus type 1 thymidine kinase gene and regional assignment of the gene for aminoacylase-1 in human chromosomes.
Hypersensitivity
Absence of metabotropic glutamate receptor homolog(s) accelerates acetylcholine neurotransmission in Caenorhabditis elegans.
Infections
ZmACY-1 Antagonistically Regulates Growth and Stress Responses in Nicotiana benthamiana.
Intellectual Disability
A comprehensive clinical and genetic study in 127 patients with ID in Kinshasa, DR Congo.
Intellectual Disability
Isolated Mild Intellectual Disability Expands the Aminoacylase 1 Phenotype Spectrum.
Liver Cirrhosis
Identification and clinical significance of an elevated level of serum aminoacylase-1 autoantibody in patients with hepatitis B virus-related liver cirrhosis.
Lung Neoplasms
Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer.
Lung Neoplasms
Isolation of two contigs of overlapping cosmids derived from human chromosomal band 3p21.1 and identification of 5 new 3p21.1 genes.
Lung Neoplasms
Lack of expression of aminoacylase-1 in small cell lung cancer. Evidence for inactivation of genes encoded by chromosome 3p.
Maple Syrup Urine Disease
Qualitative urinary organic acid analysis: 10 years of quality assurance.
methylcrotonoyl-coa carboxylase deficiency
Qualitative urinary organic acid analysis: 10 years of quality assurance.
n-acyl-aliphatic-l-amino acid amidohydrolase deficiency
A comprehensive clinical and genetic study in 127 patients with ID in Kinshasa, DR Congo.
n-acyl-aliphatic-l-amino acid amidohydrolase deficiency
Aminoacylase 1 deficiency associated with autistic behavior.
n-acyl-aliphatic-l-amino acid amidohydrolase deficiency
Aminoacylase I deficiency due to ACY1 mRNA exon skipping.
n-acyl-aliphatic-l-amino acid amidohydrolase deficiency
Aminoacylase I deficiency: a novel inborn error of metabolism.
n-acyl-aliphatic-l-amino acid amidohydrolase deficiency
Four years follow up of ACY1 deficient patient and pedigree study.
n-acyl-aliphatic-l-amino acid amidohydrolase deficiency
Gas chromatographic-mass spectrometric analysis of N-acetylated amino acids: the first case of aminoacylase I deficiency.
n-acyl-aliphatic-l-amino acid amidohydrolase deficiency
Neurological findings in aminoacylase 1 deficiency.
n-acyl-aliphatic-l-amino acid amidohydrolase deficiency
NMR spectroscopy of aminoacylase 1 deficiency, a novel inborn error of metabolism.
n-acyl-aliphatic-l-amino acid amidohydrolase deficiency
Qualitative urinary organic acid analysis: 10 years of quality assurance.
n-acyl-aliphatic-l-amino acid amidohydrolase deficiency
The molecular basis of aminoacylase 1 deficiency.
Neoplasm Metastasis
Aminoacylase 1 (ACY-1) Mediates the Proliferation and Migration of Neuroblastoma Cells in Humans Through the ERK/Transforming Growth Factor ? (TGF-?) Signaling Pathways.
Neoplasms
Cobalt-activated acylase and aminoacylase I in human malignant tumors.
Neoplasms
Differential aminoacylase expression in neuroblastoma.
Neoplasms
Effect of the ACY-1 gene on HER2 and TRAIL expression in rectal carcinoma.
Neoplasms
Genome-wide analysis identifies a tumor suppressor role for aminoacylase 1 in iron-induced rat renal cell carcinoma.
Neoplasms
Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer.
Neoplasms
Lack of expression of aminoacylase-1 in small cell lung cancer. Evidence for inactivation of genes encoded by chromosome 3p.
Neoplasms
Molecular signatures classify astrocytic gliomas by IDH1 mutation status.
Neoplasms
Proteome analysis on an early transformed human bronchial epithelial cell line, BEP2D, after alpha-particle irradiation.
Neoplasms
Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma.
Neoplasms
Study of the expression and function of ACY1 in patients with colorectal cancer.
Nervous System Diseases
Detection of N-acetyl methionine in human and murine brain and neuronal and glial derived cell lines.
Neuroblastoma
Aminoacylase 1 (ACY-1) Mediates the Proliferation and Migration of Neuroblastoma Cells in Humans Through the ERK/Transforming Growth Factor ? (TGF-?) Signaling Pathways.
Neuroblastoma
Differential aminoacylase expression in neuroblastoma.
Obesity
Attenuated metabolism is a hallmark of obesity as revealed by comparative proteomic analysis of human omental adipose tissue.
Obesity
Differential representation of liver proteins in obese human subjects suggests novel biomarkers and promising targets for drug development in obesity.
Rectal Neoplasms
Effect of the ACY-1 gene on HER2 and TRAIL expression in rectal carcinoma.
Retinoblastoma
Lack of expression of aminoacylase-1 in small cell lung cancer. Evidence for inactivation of genes encoded by chromosome 3p.
Seizures
Isolated Mild Intellectual Disability Expands the Aminoacylase 1 Phenotype Spectrum.
Small Cell Lung Carcinoma
Genetic relationship between acylpeptide hydrolase and acylase, two hydrolytic enzymes with similar binding but different catalytic specificities.
Small Cell Lung Carcinoma
Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer.
Small Cell Lung Carcinoma
Lack of expression of aminoacylase-1 in small cell lung cancer. Evidence for inactivation of genes encoded by chromosome 3p.
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168
N-2,2-dimethylpropionyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
59.9
N-acetyl-glycine
25°C, pH7.4
20.1
N-acetyl-L-alanine
25°C, pH7.4
27.7
N-acetyl-L-asparagine
25°C, pH7.4
1.4
N-acetyl-L-cysteine
25°C, pH7.4
1.28
N-acetyl-L-ethionine
25°C, pH7.4
63 - 70
N-acetyl-L-glutamate
62.3
N-acetyl-L-glutamine
25°C, pH7.4
22.7
N-acetyl-L-histidine
25°C, pH7.4
2.4
N-acetyl-L-leucine
25°C, pH7.4
0.4 - 4.32
N-acetyl-L-methionine
0.44
N-acetyl-L-norleucine
25°C, pH7.4
4.81
N-acetyl-L-phenylalanine
25°C, pH7.4
32.5
N-acetyl-L-threonine
25°C, pH7.4
11.4
N-acetyl-L-valine
25°C, pH7.4
0.41 - 1.63
N-benzoyl-L-methionine
0.73
N-butanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
1.17 - 6.23
N-cyclohexanoyl-L-methionine
7.18
N-cyclopentanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
16.8
N-formyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
1.01 - 5.22
N-hexanoyl-L-methionine
15.3
N-isobutanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.54 - 7.29
N-isopentanoyl-L-methionine
0.19
N-pentanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.34
N-propionyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.2
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
0.5
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
0.3
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
0.5
N-acetyl-L-Cys
-
analysis by HPLC, pH 7.6, 37°C
0.5
N-acetyl-L-Met
-
analysis by HPLC, pH 7.6, 37°C
0.39 - 67.4
Nalpha-acetylmethionine
additional information
additional information
-
steady-state kinetics of wild-type and mutant enzymes, kinetics of mutants N263D, R276N, and R276Q are not measurable
-
63
N-acetyl-L-glutamate
25°C, pH7.4
63
N-acetyl-L-glutamate
hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1
70
N-acetyl-L-glutamate
hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1
0.4
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant
0.43
N-acetyl-L-methionine
25°C, pH7.4
0.43
N-acetyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.76
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347F-mutant
1.14
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
1.35
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant
1.72
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant
2.76
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant
2.78
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347G-mutant
4.32
N-acetyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372G-mutant
0.41
N-benzoyl-L-methionine
hydrolysis of N-acetyl-L-methionine substrates by wild-type human Acy1
0.81
N-benzoyl-L-methionine
hydrolysis of N-acetyl-L-methionine substrates by wild-type human Acy1
1.63
N-benzoyl-L-methionine
hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1
1.17
N-cyclohexanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant
6.23
N-cyclohexanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
1.01
N-hexanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
3.56
N-hexanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
5.22
N-hexanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant
0.54
N-isopentanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant
2.1
N-isopentanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant
4.27
N-isopentanoyl-L-methionine
hydrolysis of N-acyl-L-methionine substrates by hAcy1
7.29
N-isopentanoyl-L-methionine
hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant
0.39
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant D274A
0.43
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.54
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206A
0.58
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206K
0.78
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant N263D
2.02
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206N
67.4
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant R276A
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0015
N-2,2-dimethylpropionyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
31.7
N-acetyl-glycine
25°C, pH7.4
28.1
N-acetyl-L-cysteine
25°C, pH7.4
12.4
N-acetyl-L-ethionine
25°C, pH7.4
63.4 - 156
N-acetyl-L-glutamate
30.7
N-acetyl-L-glutamine
25°C, pH7.4
1.2
N-acetyl-L-histidine
25°C, pH7.4
55.5
N-acetyl-L-leucine
25°C, pH7.4
1.2 - 93.7
N-acetyl-L-methionine
156
N-acetyl-L-norleucine
25°C, pH7.4
0.14
N-acetyl-L-phenylalanine
25°C, pH7.4
2.23
N-acetyl-L-threonine
25°C, pH7.4
14.7
N-acetyl-L-valine
25°C, pH7.4
0.0101
N-benzoyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1
0.158 - 20.1
N-benzoyl-L-methionine
139
N-butanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.00623 - 0.129
N-cyclohexanoyl-L-methionine
13.3
N-cyclopentanoyl-L-methionine
kcat the hydrolysis of N-acyl-L-methionine substrates by hAcy1
177
N-formyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.052 - 39.4
N-hexanoyl-L-methionine
0.529 - 6.08
N-isobutanoyl-L-methionine
0.105 - 5.36
N-isopentanoyl-L-methionine
16.2
N-pentanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
25.7
N-propionyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
150
2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
120
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
80
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid
-
analysis by HPLC, pH 7.6, 37°C
30
N-acetyl-L-Cys
-
analysis by HPLC, pH 7.6, 37°C
65
N-acetyl-L-Met
-
analysis by HPLC, pH 7.6, 37°C
0.006 - 38.3
Nalpha-acetylmethionine
additional information
additional information
-
kinetics of mutants N263D, R276N, and R276Q are not measurable
-
63.4
N-acetyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1-T347S-mutant
120
N-acetyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1-T347S-mutant
156
N-acetyl-L-glutamate
25°C, pH7.4
156
N-acetyl-L-glutamate
kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1
1.2
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347F-mutant
4.49
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant
12.1
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant
16.6
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372G-mutant
38.3
N-acetyl-L-methionine
25°C, pH7.4
38.3
N-acetyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
46
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347G-mutant
64.6
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant
66.7
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
93.7
N-acetyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant
0.158
N-benzoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
20.1
N-benzoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1-T347S-mutant
0.00623
N-cyclohexanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.129
N-cyclohexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant
0.052 - 2.1
N-hexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
9.51
N-hexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant
16.1
N-hexanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
39.4
N-hexanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant
0.529
N-isobutanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
6.08
N-isobutanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.105
N-isopentanoyl-L-methionine
kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1
0.34
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant
2.01
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant
3.3
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant
5.36
N-isopentanoyl-L-methionine
kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant
0.006
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant D274A
0.015
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206N
0.086
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206A
0.149
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant H206K
0.458
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant N263D
0.667
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant mutant R276A
38.3
Nalpha-acetylmethionine
-
pH 7.4, 22°C, recombinant wild-type enzyme
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L372A
2fold decreased KM-value compared to the wildtype with N-isopentanoyl-L-methionine
L372G
10fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety
T347F
1.8fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety
T347G
6.5fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety
D274A
-
site-directed mutagenesis, the mutant shows 5695fold reduced activity compared to the wild-type enzyme, but the same expression and purification behaviour
H206A
-
site-directed mutagenesis, dimerization domain mutant, the mutant shows 560fold reduced activity compared to the wild-type enzyme, but the same expression and purification behaviour
H206K
-
site-directed mutagenesis, dimerization domain mutant, the mutant shows 348fold reduced activity compared to the wild-type enzyme, but the same expression and purification behaviour
H206N
-
site-directed mutagenesis, dimerization domain mutant, the mutant shows 11837fold reduced activity compared to the wild-type enzyme
N263D
-
site-directed mutagenesis, dimerization domain mutant, nearly inactive mutant showing the same expression and purification behaviour compared to the wild-type enzyme
N263S
-
site-directed mutagenesis, dimerization domain mutant, the mutant shows 152fold reduced activity compared to the wild-type enzyme, but the same expression and purification behaviour
R276A
-
site-directed mutagenesis, dimerization domain mutant, the mutant shows 8995fold reduced activity compared to the wild-type enzyme, but the same expression and purification behaviour
R276N
-
site-directed mutagenesis, dimerization domain mutant, nearly inactive mutant showing the same expression and purification behaviour compared to the wild-type enzyme
R276Q
-
site-directed mutagenesis, dimerization domain mutant, nearly inactive mutant showing the same expression and purification behaviour compared to the wild-type enzyme
I177A
2.7fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety
I177A
3.5fold increased KM-value compared to the wildtype with N-hexanoyl-L-methionine moiety
I177L
4fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety
I177L
5.2fold increased KM-value compared to the wildtype with N-hexanoyl-L-methionine moiety
L372I
1,7fold increased KM-value compared to the wildtype with N-isopentanoyl-L-methionine moiety
L372I
3.1fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety
L372V
6.4fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety
L372V
7.9fold decreased KM-value compared to the wildtype with N-isopentanoyl-L-methionine moiety
T347S
1.1fold decreased KM-value compared to the wildtype with N-acetyl-L-methionine moiety
T347S
5.3fold decreased KM-value compared to the wildtype with N-cyclohexanoyl-L-methionine moiety
additional information
enzyme expression silencing in HCT-116 cells using a small interfering RNA
additional information
-
enzyme expression silencing in HCT-116 cells using a small interfering RNA
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Sakanyan, V.; Desmarez, L.; Legrain, C.; Charlier, D.; Mett, I.; Kochikyan, A.; Savchenko, A.; Boyen, A.; Falmagne, P.; Pierard, A.; Glansdorff, N.
Gene cloning, sequence analysis, purification, and characterization of an thermostable aminoacylase from Bacillus stearothermophilus
Appl. Environ. Microbiol.
59
3878-3888
1993
Aspergillus oryzae, Bacillus sp. (in: Bacteria), Homo sapiens, Sus scrofa
brenda
Palm, G.J.; Roehm, K.H.
Aminoacylase I from porcine kidney: Identification and characterization of two major protein domains
J. Protein Chem.
14
233-240
1995
Homo sapiens, Sus scrofa
brenda
Uttamsingh, V.; Keller, D.A.; Anders, M.W.
Acylase I-catalyzed deacetylatin of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines
Chem. Res. Toxicol.
11
801-809
1998
Homo sapiens, Rattus norvegicus, Sus scrofa
brenda
Lindner, H.A.; Alary, A.; Boju, L.I.; Sulea, T.; Menard, R.
Roles of dimerization domain residues in binding and catalysis by aminoacylase-1
Biochemistry
44
15645-15651
2005
Homo sapiens
brenda
Lindner, H.A.; Alary, A.; Wilke, M.; Sulea, T.
Probing the acyl-binding pocket of aminoacylase-1
Biochemistry
47
4266-4275
2008
Sus scrofa, Homo sapiens (Q03154), Homo sapiens
brenda
Lindner, H.A.; Taefler-Naumann, M.; Roehm, K.H.
N-acetylamino acid utilization by kidney aminoacylase-1
Biochimie
90
773-780
2008
Homo sapiens (Q03154), Homo sapiens, Sus scrofa (P37111), Sus scrofa
brenda
Tsirulnikov, K.; Abuladze, N.; Newman, D.; Ryazantsev, S.; Wolak, T.; Magilnick, N.; Koag, M.C.; Kurtz, I.; Pushkin, A.
Mouse aminoacylase 3: a metalloenzyme activated by cobalt and nickel
Biochim. Biophys. Acta
1794
1049-1057
2009
Homo sapiens, Mus musculus
brenda
Stocker, P.; Brunel, J.; de Rezende, L.; -do Amaral, A.; Morelli, X.; Roche, P.; Vidal, N.; Giardina, T.; Perrier, J.
Aminoacylase 1-catalysed deacetylation of bioactives epoxides mycotoxin-derived mercapturates; 3,4-epoxyprecocenes as models of cytotoxic epoxides
Biochimie
94
1668-1675
2012
Homo sapiens, Sus scrofa (P37111), Sus scrofa
brenda
Shi, H.; Hayes, M.T.; Kirana, C.; Miller, R.J.; Keating, J.P.; Stubbs, R.S.
Overexpression of aminoacylase 1 is associated with colorectal cancer progression
Hum. Pathol.
44
1089-1097
2013
Homo sapiens (Q03154), Homo sapiens
brenda
Anila, A.H.; Ali, F.; Kushwaha, S.; Taye, N.; Chattopadhyay, S.; Das, A.
A cysteine-specific fluorescent switch for monitoring oxidative stress and quantification of aminoacylase-1 in blood serum
Anal. Chem.
88
12161-12168
2016
Homo sapiens (Q03154)
brenda