Information on EC 3.5.1.126 - oxamate amidohydrolase

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The expected taxonomic range for this enzyme is: Klebsiella pneumoniae subsp. pneumoniae

EC NUMBER
COMMENTARY hide
3.5.1.126
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RECOMMENDED NAME
GeneOntology No.
oxamate amidohydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
oxamate + H2O = oxalate + ammonia
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
oxamate amidohydrolase
The enzyme has been characterized from the bacterium Klebsiella pneumoniae.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
oxamate + H2O
oxalate + ammonia
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
oxamate + H2O
oxalate + ammonia
show the reaction diagram
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.5
oxamate
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pH 8.5, 22°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.159
oxamate
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pH 8.5, 22°C
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to 2.9 A resolution. This active site is located in a solvent-exposed cleft with nonpolar amino acids lining the binding cleft, including Met423, Gly424 and Gly425. Other amino acids in the active site are Thr342, Ser360, Tyr362 and Leu405
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene hpxU encodes a repressor that mediates the regulation of the hpxWYXZ cluster. Nitrogen limitation results in weak repression of repressor HpxU expression and weak activation of HpxW expression. The addition of allantoin or allantoate to nitrogen-limiting cultures does not significantly modify HpxW expression, but presence of oxamate leads to 50% increase in expression
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S360A
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mutant is able to autoprocess, but is catalytically inactive
T342A
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mutant does not autoprocess into a mature heterodimer and is inactive
S360A
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mutant is able to autoprocess, but is catalytically inactive
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T342A
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mutant does not autoprocess into a mature heterodimer and is inactive
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