Information on EC 3.5.1.120 - 2-aminomuconate deaminase (2-hydroxymuconate-forming)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.120
-
RECOMMENDED NAME
GeneOntology No.
2-aminomuconate deaminase (2-hydroxymuconate-forming)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-aminomuconate + H2O = (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
show the reaction diagram
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
2-aminomuconate aminohydrolase [(2Z,4E)-2-hydroxyhexa-2,4-dienedioate-forming]
The enzyme, characterized from the bacterium Comamonas testosteroni CNB-1, converts 2-aminomuconate to 2-hydroxyhexa-2,4-dienedioate, unlike the enzymes from Pseudomonas, which produce (3E)-2-oxohex-3-enedioate (see EC 3.5.99.5, 2-aminomuconate deaminase). The enzyme also acts on 2-amino-5-chloromuconate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-amino-5-chloromuconate + H2O
2-hydroxy-5-chloromuconate + NH3
show the reaction diagram
2-aminomuconate + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
show the reaction diagram
2-aminomuconate + H2O
4-oxalocrotonate + NH3
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-aminomuconate + H2O
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NH3
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diethyl dicarbonate
Mn2+
2 mM, 20% inhibition
Ni2+
-
2 mM, complete inhibition
p-chloromercuribenzoate
-
0.2 mM, complete inhibition
phenylhydrazine
10 mM, 80% inhibition
additional information
not inhibitory: saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid, or EDTA at 25 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0088
2-amino-5-chloromuconate
-
pH and temperature not specified in the publication
-
0.0039 - 0.067
2-aminomuconate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
208
2-aminomuconate
Pseudomonas pseudoalcaligenes
P81593
pH 6.6, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.147
-
substrate: 2-aminomuconate, pH and temperature not specified in the publication
0.196
-
substrate: 2-amino-5-chloromuconate, pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
1 * 28000, SDS-PAGE
30000
-
gel filtration
100000
gel filtration and PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7 - 8.8
room temperature, stable for at least 3 h
172186
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
stable at room temperature for 3 days
60
5 min, 23% residual activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE