Information on EC 3.5.1.115 - mycothiol S-conjugate amidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.115
-
RECOMMENDED NAME
GeneOntology No.
mycothiol S-conjugate amidase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a mycothiol S-conjugate + H2O = an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mycothiol-mediated detoxification
-
-
SYSTEMATIC NAME
IUBMB Comments
mycothiol S-conjugate 1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranosyl-hydrolase
The enzyme that is found in actinomycetes is involved in the detoxification of oxidizing agents and electrophilic antibiotics. The enzyme has low activity with 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol as substrate (cf. EC 3.5.1.103, N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase) [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
RES167, gene mca or NCgl0948
UniProt
Manually annotated by BRENDA team
RES167, gene mca or NCgl0948
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
-
mycothiol S-conjugate amidase plays an important role in the detoxification of alkylating agents and antibiotics
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
a mycothiol S-conjugate + H2O
an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
show the reaction diagram
mycothiol bimane + H2O
?
show the reaction diagram
-
-
-
-
?
mycothiol bimane + H2O
mycothiol + bimane
show the reaction diagram
mycothiol bimane derivative + H2O
?
show the reaction diagram
-
100% activity
-
-
?
mycothiol disulfide + H2O
?
show the reaction diagram
mycothiol-3-(N-maleimidopropionyl)biocytin + H2O
?
show the reaction diagram
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin + H2O
?
show the reaction diagram
mycothiol-acetophenone + H2O
?
show the reaction diagram
-
-
-
-
?
mycothiol-cerulenin + H2O
?
show the reaction diagram
mycothiol-monobromobimane + H2O
? + 1D-myo-inosityl 2-amino-2-deoxy-glucopyranoside
show the reaction diagram
mycothiol-N-ethylmaleimide + H2O
?
show the reaction diagram
mycothiol-rifamycin S + H2O
?
show the reaction diagram
RifS13 + H2O
1-D-myo-inositol-alpha-D-glucopyranoside + N-acetyl-S-[(2R,12Z,14E,16S,18S,19S,20S,21S,22S,23S,24E)-5,6,9,17,19-pentahydroxy-23-methoxy-21-(methoxycarbonyl)-2,4,12,16,18,20,22-heptamethyl-1,11-dioxo-1,2-dihydro-2,7-(epoxypentadeca[1,11,13]trienoimino)naphtho[2,1-b]furan-8-yl]-L-cysteine
show the reaction diagram
-
-
-
-
?
RifS17 + H2O
1-D-myo-inositol-alpha-D-glucopyranoside + N-acetyl-S-[(2R,12Z,14E,16S,18S,19S,20S,21S,22S,23S,24E)-5,17,19-trihydroxy-23-methoxy-21-(methoxycarbonyl)-2,4,12,16,18,20,22-heptamethyl-1,6,9,11-tetraoxo-1,2,6,9-tetrahydro-2,7-(epoxypentadeca[1,11,13]trienoimino)naphtho[2,1-b]furan-8-yl]-L-cysteine
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a mycothiol S-conjugate + H2O
an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
show the reaction diagram
mycothiol bimane + H2O
mycothiol + bimane
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
the protein contains 2.6 equivalents of Ca2+ per subunit after purification
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-({[3-chloro-4-(propan-2-ylsulfonyl)thiophen-2-yl]carbonyl}amino)-2-deoxy-alpha-D-glucopyranoside
-
11% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-({[1-(3,5-dichlorophenyl)-5-ethyl-1H-pyrazol-4-yl]carbonyl}amino)-alpha-D-glucopyranoside
-
26% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-[(4-nitrobenzoyl)amino]-alpha-D-glucopyranoside
-
15% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-[(furan-2-ylcarbonyl)amino]-alpha-D-glucopyranoside
-
15% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-[(naphthalen-2-ylcarbonyl)amino]-alpha-D-glucopyranoside
-
13% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-[(pyridin-4-ylcarbonyl)amino]-alpha-D-glucopyranoside
-
46% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-[(thiophen-2-ylcarbonyl)amino]-alpha-D-glucopyranoside
-
13% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-{[3-(propylsulfanyl)benzoyl]amino}-alpha-D-glucopyranoside
-
less than 10% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-{[4-(dimethylamino)benzoyl]amino}-alpha-D-glucopyranoside
-
44% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-{[4-(trifluoromethyl)benzoyl]amino}-alpha-D-glucopyranoside
-
16% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-[(1-benzothiophen-6-ylcarbonyl)amino]-2-deoxy-alpha-D-glucopyranoside
-
15% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-[(cyclopropylcarbonyl)amino]-2-deoxy-alpha-D-glucopyranoside
-
13% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-[({6-[(4-chlorophenyl)sulfanyl]pyridin-2-yl}carbonyl)amino]-2-deoxy-alpha-D-glucopyranoside
-
10% inhibition at 0.05 mM
(2E)-3-(3,5-difluorophenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
-
-
(2E)-3-(3-bromophenyl)-N-[2-[(2,6-dimethylphenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
-
-
(2E)-3-(3-bromophenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
-
-
(2E)-3-(3-chloro-4-hydroxyphenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
-
-
(2E)-N-(5-amino-5-iminopentyl)-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
-
-
(2E)-N-[2-(2-amino-1H-imidazol-5-yl)ethyl]-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
-
-
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3,5-difluorophenyl)-2-(hydroxyimino)propanamide
-
-
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3-bromophenyl)-2-(hydroxyimino)propanamide
-
-
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3-chloro-4-hydroxyphenyl)-2-(hydroxyimino)propanamide
-
-
(2S,3R,26S,27S)-2,27-diamino-1,3,26-trihydroxyoctacosan-13-one
(2Z)-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-N-(4-carbamimidamidobutyl)-2-(hydroxyimino)propanamide
-
-
(2Z)-N-(4-amino-4-iminobutyl)-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
(2Z)-N-[(2-amino-1H-imidazol-5-yl)methyl]-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
(2Z)-N-[2-(2-amino-1H-imidazol-5-yl)ethyl]-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
-
-
(5R,10S)-N-[2-(2-amino-1H-imidazol-5-yl)ethyl]-7,9-dibromo-10-hydroxy-8-methoxy-1-oxa-2-azaspiro[4.5]deca-2,6,8-triene-3-carboxamide
(5R,10S)-N-[2-[2-amino-4-(3,5,8-trihydroxy-4-oxo-1,4-dihydroquinolin-6-yl)-1H-imidazol-5-yl]ethyl]-7,9-dibromo-10-hydroxy-8-methoxy-1-oxa-2-azaspiro[4.5]deca-2,6,8-triene-3-carboxamide
-
-
(5R,10S)-N-[2-[2-amino-4-(3,5,8-trihydroxy-4-oxo-1,4-dihydroquinolin-6-yl)-1H-imidazol-5-yl]ethyl]}-7,9-dibromo-10-hydroxy-8-methoxy-1-oxa-2-azaspiro[4.5]deca-2,6,8-triene-3-carboxamide
-
strongest inhibitor
1,10-phenanthroline
1,7-phenanthroline
-
slightly more than 50% inhibition at 1 mM 1,7-phenanthroline
1-pentyl-2-[10-(3-pentylpyridinium-1-yl)decyl]pyridinium
3,5,8-trihydroxyquinolin-4(1H)-one
-
-
bromotyrosine oxime
-
-
disodium 3-[(E)-2-carboxylatoethenyl]benzoate
gliotoxin
halisulfate 1
hemibastidin-2
-
-
mycothiol
oceanapiside
physcion
psammaplysin A
psammaplysin B
pseudoceratine
-
-
S,S-dimethyl gliotoxin
suvanine
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
92.34 - 542.4
mycothiol bimane
0.095
mycothiol bimane derivative
-
at pH 7.5 and 30C
4.8
mycothiol disulfide
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
3
mycothiol-3-(N-maleimidopropionyl)biocytin
-
Km above 3.0 mM, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
0.44
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
0.69
mycothiol-acetophenone
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
0.65
mycothiol-cerulenin
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
1.3
mycothiol-N-ethylmaleimide
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
0.19
RifS13
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
0.45
RifS17
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0048 - 0.078
mycothiol bimane
8
mycothiol bimane derivative
Mycobacterium smegmatis
-
at pH 7.5 and 30C
1.7
mycothiol disulfide
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
3
mycothiol-3-(N-maleimidopropionyl)biocytin
Mycobacterium tuberculosis
-
kcat above 3.0 s-1, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
1.3
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
37
mycothiol-acetophenone
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
7
mycothiol-cerulenin
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
15
mycothiol-N-ethylmaleimide
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
0.82
RifS13
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
1.04
RifS17
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.35
mycothiol disulfide
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
42666
1.03
mycothiol-3-(N-maleimidopropionyl)biocytin
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
42670
3
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
42669
54
mycothiol-acetophenone
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
42667
11
mycothiol-cerulenin
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
42668
12
mycothiol-N-ethylmaleimide
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
42671
4.3
RifS13
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
42672
2.3
RifS17
Mycobacterium tuberculosis
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
42673
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.72
(2E)-3-(3,5-difluorophenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.065
(2E)-3-(3-bromophenyl)-N-[2-[(2,6-dimethylphenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.09
(2E)-3-(3-bromophenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.037
(2E)-3-(3-chloro-4-hydroxyphenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.0028
(2E)-N-(5-amino-5-iminopentyl)-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.036
(2E)-N-[2-(2-amino-1H-imidazol-5-yl)ethyl]-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.45
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3,5-difluorophenyl)-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.185
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3-bromophenyl)-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.035
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3-chloro-4-hydroxyphenyl)-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.05 - 0.1
(2S,3R,26S,27S)-2,27-diamino-1,3,26-trihydroxyoctacosan-13-one
0.002 - 0.003
(2Z)-N-(4-amino-4-iminobutyl)-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
0.03 - 0.037
(2Z)-N-[(2-amino-1H-imidazol-5-yl)methyl]-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
0.1
(5R,10S)-N-[2-(2-amino-1H-imidazol-5-yl)ethyl]-7,9-dibromo-10-hydroxy-8-methoxy-1-oxa-2-azaspiro[4.5]deca-2,6,8-triene-3-carboxamide
0.002
(5R,10S)-N-[2-[2-amino-4-(3,5,8-trihydroxy-4-oxo-1,4-dihydroquinolin-6-yl)-1H-imidazol-5-yl]ethyl]-7,9-dibromo-10-hydroxy-8-methoxy-1-oxa-2-azaspiro[4.5]deca-2,6,8-triene-3-carboxamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.0001
1-pentyl-2-[10-(3-pentylpyridinium-1-yl)decyl]pyridinium
0.03
bromotyrosine oxime
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.002 - 0.003
disodium 3-[(E)-2-carboxylatoethenyl]benzoate
0.05
gliotoxin
0.04
halisulfate 1
Mycobacterium smegmatis
-
at 31C, pH not specified in the publication
0.0005 - 0.01
oceanapiside
0.05
physcion
Mycobacterium smegmatis
-
at 31C, pH not specified in the publication
0.0028
psammaplin A
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.02 - 0.03
psammaplysin A
0.02 - 0.03
psammaplysin B
0.1
pseudoceratine
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.07
S,S-dimethyl gliotoxin
Mycobacterium tuberculosis
-
at 31C, pH not specified in the publication
0.06
suvanine
Mycobacterium smegmatis
-
at 31C, pH not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0012
-
crude extract, at pH 7.5 and 30C
0.13
-
crude extract, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
3.3
-
after 2800fold purification, at pH 7.5 and 30C
10.6
-
after 82fold purification, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
recombinant enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32697
-
x * 32697, calculated from amino acid sequence
33000
-
x * 33000, SDA-PAGE
35000
recombinant enzyme, gel filtration
36000
-
x * 36000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
-
no significant dependence upon pH is detected from pH 7.0 to 9.0, but the activity declines sharply below pH 7.0. At pH 7.5, activity is about 25% lower in 50 mM sodium phosphate than in 25 mM HEPES
724260
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Sephadex G-25 gel filtration
-
ammonium sulfate precipitation, Toyopearl DEAE 650C column chromatography, phenyl Sepharose and Sephadex G-100 gel filtration
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) to homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
gene mca or NCgl0948, quantitative real-time PCR enzyme expression analysis, recombinant wild-type and mutant enzyme expression in Escherichia coli strain BL21(DE3)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
induced expression of Mca in Corynebacterium glutamicum under various stress conditions, directly under the control of the stress-responsive extracytoplasmic function-sigma (ECF-s) factor SigH, positive regulation of mca expression by SigH
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D132A
site-directed mutagenesis, the mutant shows slightly decreased activity compared to the wild-type enzyme
D141A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
D14A
site-directed mutagenesis, the mutant shows slightly decreased activity compared to the wild-type enzyme
D15A
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme
E16A
site-directed mutagenesis, the mutant shows slightly decreased activity compared to the wild-type enzyme
E43A
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme
H10A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
H12A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
H139A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
H142A
site-directed mutagenesis, the mutant shows slightly decreased activity compared to the wild-type enzyme
Y137A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
D132A
-
site-directed mutagenesis, the mutant shows slightly decreased activity compared to the wild-type enzyme
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D141A
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site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
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D14A
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site-directed mutagenesis, the mutant shows slightly decreased activity compared to the wild-type enzyme
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H139A
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site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
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additional information
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