Reference on EC 3.5.1.11 - penicillin amidase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bauer, K.; Kaufmann, W.; Ludwig, S.A.
A simplified determination of penicillin amidase from E. coli
Hoppe-Seyler's Z. Physiol. Chem.
352
1723-1724
1971
Escherichia coli
Balasingham, K.; Warburton, O.; Dunnil, P.; Lilly, M.D.
The isolation and kinetics of penicillin amidase from Escherichia coli
Biochim. Biophys. Acta
276
250-256
1972
Escherichia coli
Warburton, D.; Balasingham, K.; Dunnil, P.; Lilly, M.D.
The preparation and kinetics of immobilised penicillin amidase from Escherichia coli
Biochim. Biophys. Acta
284
278-284
1972
Escherichia coli
Boeck, A.; Wirth, R.; Schmid, G.; Schumacher, G.; Lang, G.; Buckel, P.
The penicillin acylase from Escherichia coli ATCC 11105 consists of two dissimilar subunits
FEMS Microbiol. Lett.
20
135-139
1983
Escherichia coli
-
Berezin, I.V.; Klibanov, A.M.; Klyosov, A.A.; Martinek, K.; Svedas, V.K.
The effect of ultrasound as a new method of studying conformational transitions in enzyme active sites. pH- and temperature-induced conformational transitions in the active centre of penicillin amidase
FEBS Lett.
49
325-328
1975
Escherichia coli
Cole, M.; Savidge, T.; vander Haeghe, H.
Penicillin acylase (assay)
Methods Enzymol.
43
698-705
1975
Bacillus subtilis, Escherichia coli
Savidge, T.A.; Cole, M.
Penicillin acylase (bacterial)
Methods Enzymol.
43
705-721
1975
Priestia megaterium, Escherichia coli
vander Haeghe, H.
Penicillin acylase (fungal)
Methods Enzymol.
43
721-728
1975
Fusarium sp., Penicillium chrysogenum
Szewczuk, A.; Ziomek, E.; Mordarski, M.; Siewinski, M.; Wieczorek, J.
Properties of penicillin amidase immobilized by copolymerization with acrylamide
Biotechnol. Bioeng.
21
1543-1552
1979
Escherichia coli
Szewczuk, A.; Siewinski, M.; Slowinska, R.
Colorimetric assay of penicillin amidase activity using phenylacetyl-aminobenzoic acid as substrate
Anal. Biochem.
103
166-169
1980
Escherichia coli
Morgolin, A.L.; Svedas, V.K.; Berezin, I.V.
Substrate specificity of penicillin amidase from E. coli
Biochim. Biophys. Acta
616
283-289
1980
Escherichia coli
Svedas, V.K.; Margolin, A.L.; Borisov, I.L.; Berezin, I.V.
Kinetics of the enzymatic synthesis of benzylpenicillin
Enzyme Microb. Technol.
2
313-317
1980
Escherichia coli
-
McDougall, B.; Dunnill, P.; Lilly, M.D.
Enzymatic acylation of 6-aminopenicillanic acid
Enzyme Microb. Technol.
4
114-115
1982
Escherichia coli
-
Siewinski, M.; Kuropatwa, M.; Szewczuk, A.
Phenylalkylsulfonyl derivatives as covalent inhibitors of penicillin amidase
Hoppe-Seyler's Z. Physiol. Chem.
365
829-837
1984
Escherichia coli
Kasche, V.; Haufler, U.; Zllner, R.
Kinetic studies on the mechanism of the penicillin amidase-catalysed synthesis of ampicillin and benzylpenicillin
Hoppe-Seyler's Z. Physiol. Chem.
365
1435-1443
1984
Escherichia coli
Kasche, V.
Ampicillin- and cephalhexin-synthesis catalyzed by E. coli penicillin amidase. yield increase due to substrate recycling.
Biotechnol. Lett.
7
877-882
1985
Escherichia coli
-
Sudhakaran, V.K.; Shewale, J.G.
Hydrophobic interaction chromatography of penicillin amidase
Biotechnol. Lett.
9
539-542
1987
Escherichia coli
-
Brandl, E.
Information on penicillin amidase
Hoppe-Seyler's Z. Physiol. Chem.
342
86
1965
Escherichia coli
Self, D.A.; Kay, G.; Lilly, M.D.; Dunnill, P.
The conversion of benzyl penicillin to 6-aminopenicillanic acid using an insoluble derivative of penicillin amidase
Biotechnol. Bioeng.
11
337
1969
Escherichia coli
Kutzbach, C.; Rauenbusch, E.
Preparation and general properties of crystalline penicillin acylase from Escherichia coli ATCC 11 105
Hoppe-Seyler's Z. Physiol. Chem.
355
45-53
1974
Escherichia coli
Nys, P.S.; Kolygina, T.S.; Garaev, M.M.
Penicillin amidase from E. coli. A direct spectrophotometric method of determining the enzymes activity
Antibiotiki
22
211-216
1977
Escherichia coli
Margolin, A.L.; Izumrudov, V.A.; Svedas, V.K.; Zezin, A.B.; Kabanov, V.A.; Berezin, I.V.
Preparation and properties of penicillin amidase immobilized in polyelectrolyte complexes
Biochim. Biophys. Acta
660
359-365
1981
Escherichia coli
Kim, D.J.; Byun S.M.
Purification and properties of ampicillin acylase from Pseudomonas melanogenum
Biochim. Biophys. Acta
1040
12-18
1990
Pseudomonas melanogenum
Prieto, I.; Martin J.; Arche R.; Fernandez P.; Perez-Aranda A.; Barbero J.L.
Penicillin acylase mutants with altered site-directed activity from Kluyvera citrophila
Appl. Microbiol. Biotechnol.
33
553-559
1990
Kluyvera cryocrescens
Niersbach, H.; Kuhne, A.; Tischer, W.; Weber, M.; Wedekind F.; Plapp, R.
Improvement of the catalytic properties of penicillin G acylase from Escherichia coli ATCC 11105 by selection of a new substrate specificity
Appl. Microbiol. Biotechnol.
43
679-684
1995
Escherichia coli
Sudhakaran, V.K.; Shewale, J.G.
Purification and characterization of extracellular penicillin V acylase from Fusarium sp. SKF 235
Hindustan Antibiot. Bull.
37
9-15
1995
Fusarium sp.
Sriubolmas, N.; Panbangred, W.; Sriurairatana, S.; Meevootisom, V.
Localization and characterization of inclusion bodies in recombinant Escherichia coli cells overproducing penicillin G acylase
Appl. Microbiol. Biotechnol.
47
373-378
1974
Escherichia coli
Fernandez-Lafuente, R.; Rosell, C.M.; Guisan, J.M.
Enzyme reaction engineering: synthesis of antibiotics catalysed by stabilized penicillin G acylase in the predence of organic solvents
Enzyme Microb. Technol.
13
898-905
1991
Escherichia coli, Kluyvera cryocrescens
Erarslan, A.; Terzi, I.; Guray, A.; Bermek, E.
Purification and kinetics of penicillin G acylase from a mutant strain of Escherichia coli ATCC 11105
J. Chem. Technol. Biotechnol.
51
27-40
1991
Escherichia coli
-
Erarlan, A.; Guray, A.
Kinetic investigation of penicillin G acylase from a mutant strain of Escheria coli ATCC 11105 immobilized on oxirane-acrylic beads
J. Chem. Technol. Biotechnol.
51
181-195
1991
Escherichia coli
Hunt, P.D.; Tolley, S.P.; Ward, R.J.; Hill, C.P.; Dodson, G.G.
Expression, purification and crystallization of penicillin G acylase from Escherichia coli ATCC 11105
Protein Eng.
3
635-639
1990
Escherichia coli
Pundle, A.; SivaRaman, H.
Bacillus sphaericus penicillin V acylase: purification, substrate specificity, and active-site characterization
Curr. Microbiol.
34
144-148
1997
Lysinibacillus sphaericus
Chauhan, S.; Nichkawade, A.; Iyengar, M.R.S.; Chattoo, B.B.
Chainia penicillin V acylase: strain characteristics, enzyme immobilization, and kinetic studies
Curr. Microbiol.
37
186-190
1998
Streptomyces sp., Streptomyces sp. Chainia
Azevedo, A.M.; Fonseca, L.P.; Prazeres, D.M.F.
Stability and stabilisation of penicillin acylase
J. Chem. Technol. Biotechnol.
74
1110-1116
1999
Escherichia coli
-
McDonough, M.A.; Klei, H.E.; Kelly, J.A.
Crystal structure of penicillin G acylase from the Bro1 mutant strain of Providencia rettgeri
Protein Sci.
8
1971-1981
1999
Providencia rettgeri, Providencia rettgeri Bro1
Ignatova, Z.; Mahsunah, A.; Georgieva, M.; Kasche, V.
Improvement of posttranslational bottlenecks in the production of penicillin amidase in recombinant Escherichia coli strains
Appl. Environ. Microbiol.
69
1237-1245
2003
Escherichia coli
Stambolieva, N.; Mincheva, Z.; Galunsky, B.
Kinetic comparison of penicillin amidase catalsed transfer of nonspecific and specific acyl moieties to 7-aminodeacetoxycephalosporanic acid
Biocatal. Biotransform.
16
225-232
1998
Escherichia coli
-
Kasche, V.; Lummer, K.; Nurk, A.; Piotraschke, E.; Rieks, A.; Stoeva, S.; Voelter, W.
Intramolecular autoproteolysis initiates the maturation of penicillin amidase from Escherichia coli
Biochim. Biophys. Acta
1433
76-86
1999
Escherichia coli
Dineva, M.A.; Gatunsky, B.; Kasche, V.; Petkov, D.D.
Phenylacetyl group as enzyme-cleavable aminoprotection of purine nucleosides
Bioorg. Med. Chem. Lett.
3
2781-2784
1993
Escherichia coli
-
Piotraschke, E.; Nurk, A.; Galunsky, B.; Kasche, V.
Genetic construction of catalytically active cross-species heterodimer penicillin G amidase
Biotechnol. Lett.
16
119-124
1994
Escherichia coli, Kluyvera cryocrescens
-
Kasche, V.; Galunsky, B.; Nurk, E.; Piotraschke, E.; Rieks, A.
The dependency of the stereoselectivity of penicillin amidases enzymes with R-specific S1 and S-specific S'1-subsites on temperature and primary structure
Biotechnol. Lett.
18
455-460
1996
Escherichia coli
-
Ignatova, Z.; Stoeva, S.; Galunsky, B.; Hrnle, C.; Nurk, A.; Piotraschke, E.; Voelter, W.; Kasche, V.
Proteolytic processing of penicillin amidase from Alcaligenes faecalis cloned in Escherichia coli yields several active forms
Biotechnol. Lett.
20
977-982
1998
Alcaligenes faecalis, Escherichia coli
-
Ignatova, Z.; Taruttis, S.; Kasche, V.
Role of the intracellular proteolysis in the production of the periplasmic penicillin amidase in Escherichia coli
Biotechnol. Lett.
22
1727-1732
2000
Escherichia coli
-
Ignatova, Z.; Enfors, S.O.; Hobbie, M.; Taruttis, S.; Vogt, C.; Kasche, V.
The relative importance of intracellular proteolysis and transport on the yield of the periplasmic enzyme penicillin amidase in Escherichia coli
Enzyme Microb. Technol.
26
165-170
2000
Escherichia coli
Kasche, V.; Galunsky, B.; Ignatova, Z.
Fragments of pro-peptide activate mature penicillin amidase of Alcaligenes faecalis
Eur. J. Biochem.
270
4721-4728
2003
Alcaligenes faecalis
Hewitt, L.; Kasche, V.; Lummer, K.; Lewis, R.J.; Murshudov, G.N.; Verma, C.S.; Dodson, G.G.; Wilson, K.S.
Structure of a slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active-site cleft
J. Mol. Biol.
302
887-898
2000
Escherichia coli
Galunsky, B.; Lummer, K.; Kasche, V.
Comparative study of substrate- and stereospecificity of penicillin G amidases from different sources and hybrid isoenzymes
Monatsh. Chem.
131
623-632
2000
Alcaligenes faecalis, Rhizobium viscosum, Escherichia coli, Kluyvera cryocrescens
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Tramper, J.
Chemical versus biochemical conversion: when and how to use biocatalysts
Biotechnol. Bioeng.
52
290-295
1996
Escherichia coli
Hernandez-Justiz, O.; Fernandez-Lafuente, R.; Terrini, M.; Guisan, J.M.
Use of aqueous two-phase systems for in situ extraction of water soluble antibiotics during their synthesis by enzymes immobilized on porous supports
Biotechnol. Bioeng.
59
73-79
1998
Escherichia coli
Zaks, A.; Dodds, D.R.
Application of biocatalysts and biotransformation to the synthesis of pharmaceuticals
Drug Discov. Today
2
513-531
1997
Priestia megaterium
-
Chandra, P.M.; Brannigan, J.A.; Prabhune, A.; Pundle, A.; Turkenburg, J.P.; Dodson, G.G.; Suresh, C.G.
Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants
Acta Crystallogr. Sect. F
F61
124-127
2005
Lysinibacillus sphaericus
Rathinaswamy, P.; Pundle, A.V.; Prabhune, A.A.; SivaRaman, H.; Brannigan, J.A.; Dodson, G.G.; Suresh, C.G.
Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis
Acta Crystallogr. Sect. F
F61
680-683
2005
Bacillus subtilis
Basso, A.; Ebert, C.; Gardossi, L.; Linda, P.; Phuong, T.T.; Zhu, M.; Wessjohann, L.
Penicillin G amidase-catalysed hydrolysis of phenylacetic hydrazides on a solid phase: A new route to enzyme-cleavable linkers
Adv. Synth. Catal.
347
963-966
2005
Escherichia coli
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Abian, O.; Grazu, V.; Hermoso, J.; Gonzalez, R.; Garcia, J.L.; Fernandez-Lafuente, R.; Guisan, J.M.
Stabilization of penicillin G acylase from Escherichia coli: site-directed mutagenesis of the protein surface to increase multipoint covalent attachment
Appl. Environ. Microbiol.
70
1249-1251
2004
Escherichia coli
Cai, G.; Zhu, S.; Yang, S.; Zhao, G.; Jiang, W.
Cloning, overexpression, and characterization of a novel thermostable penicillin G acylase from Achromobacter xylosoxidans: probing the molecular basis for its high thermostability
Appl. Environ. Microbiol.
70
2764-2770
2004
Achromobacter xylosoxidans (Q83YY8), Achromobacter xylosoxidans
Scaramozzino, F.; Estruch, I.; Rossolillo, P.; Terreni, M.; Albertini, A.M.
Improvement of catalytic properties of Escherichia coli penicillin G acylase immobilized on glyoxyl agarose by addition of a six-amino-acid tag
Appl. Environ. Microbiol.
71
8937-8940
2005
Escherichia coli
Oh, B.; Kim, K.; Park, J.; Yoon, J.; Han, D.; Kim, Y.
Modifying the substrate specificity of penicillin G acylase to cephalosporin acylase by mutating active-site residues
Biochem. Biophys. Res. Commun.
319
486-492
2004
Escherichia coli
Guranda, D.T.; Volovik, T.S.; Svedas, V.K.
pH Stability of penicillin acylase from Escherichia coli
Biochemistry (Moscow)
69
1386-1390
2004
Escherichia coli
Liu, S.L.; Wei, D.Z.; Song, Q.X.; Zhang, Y.W.; Wang, X.D.
Effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin G acylase from Kluyvera citrophila
Bioprocess Biosyst. Eng.
28
285-289
2006
Kluyvera cryocrescens
Masarova, J.; Mislovicova, D.; Mendichi, R.; Svitel, J.; Gemeiner, P.; Danielsson, B.
Mannan-penicillin G acylase neoglycoproteins and their potential applications in biotechnology
Biotechnol. Appl. Biochem.
39
285-291
2004
Escherichia coli
Youshko, M.I.; Moody, H.M.; Bukhanov, A.L.; Boosten, W.H.; Svedas, V.K.
Penicillin acylase-catalyzed synthesis of beta-lactam antibiotics in highly condensed aqueous systems: beneficial impact of kinetic substrate supersaturation
Biotechnol. Bioeng.
85
323-329
2004
Escherichia coli
Kumar, R.S.; Suresh, C.G.; Pundle, A.; Prabhune, A.
Evidence for the involvement of arginyl residue at the active site of penicillin G acylase from Kluyvera citrophila
Biotechnol. Lett.
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2004
Kluyvera cryocrescens
Xu, Y.; Hsieh, M.Y.; Narayanan, N.; Anderson, W.A.; Scharer, J.M.; Moo-Young, M.; Chou, C.P.
Cytoplasmic overexpression, folding, and processing of penicillin acylase precursor in Escherichia coli
Biotechnol. Prog.
21
1357-1365
2005
Escherichia coli
Wu, W.B.; Wang, N.; Xu, J.M.; Wu, Q.; Lin, X.F.
Penicillin G acylase catalyzed Markovnikov addition of allopurinol to vinyl ester
Chem. Commun. (Camb.)
2005
2348-2350
2005
Escherichia coli
Gabor, E.M.; de Vries, E.J.; Janssen, D.B.
A novel penicillin acylase from the environmental gene pool with improved synthetic properties
Enzyme Microb. Technol.
36
182-190
2005
Escherichia coli (P06875), uncultured Gammaproteobacteria bacterium (Q6PWR5)
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Terreni, M.; Ubiali, D.; Pagani, G.; Hernandez-Justiz, O.; Fernandez-Lafuente, R.; Guisan, J.M.
Penicillin G acylase catalyzed acylation of 7-ACA in aqueous two-phase systems using kinetically and thermodynamically controlled strategies: improved enzymatic synthesis of 7-[(1-hydroxy-1-phenyl)-acetamido]-3-acetoxymethyl-D3-cephem-4-carboxylic acid
Enzyme Microb. Technol.
36
672-679
2005
Escherichia coli
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Wen, Y.; Feng, M.; Yuan, Z.; Zhou, P.
Expression and overproduction of recombinant penicillin G acylase from Kluyvera citrophila in Escherichia coli
Enzyme Microb. Technol.
37
233-237
2005
Kluyvera cryocrescens
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Guncheva, M.; Ivanov, I.; Galunsky, B.; Stambolieva, N.; Kaneti, J.
Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263
Eur. J. Biochem.
271
2272-2279
2004
Escherichia coli
Viegas, S.C.; Schmidt, D.; Kasche, V.; Arraiano, C.M.; Ignatova, Z.
Effect of the increased stability of the penicillin amidase mRNA on the protein expression levels
FEBS Lett.
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5069-5073
2005
Escherichia coli
Kim, J.; Kang, H.; Kim, E.; Kim, J.; Koo, Y.
One-step purification of poly-His tagged penicillin G acylase expressed in E. coli
J. Microbiol. Biotechnol.
14
231-236
2004
Escherichia coli
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Ribeiro, M.P.; Ferreira, A.L.; Giordano, R.L.; Giordano, R.C.
Selectivity of the enzymatic synthesis of ampicillin by E. coli PGA in the presence of high concentrations of substrates
J. Mol. Catal. B
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81-86
2005
Escherichia coli
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Alkema, W.B.; Hensgens, C.M.; Snijder, H.J.; Keizer, E.; Dijkstra, B.W.; Janssen, D.B.
Structural and kinetic studies on ligand binding in wild-type and active-site mutants of penicillin acylase
Protein Eng. Des. Sel.
17
473-480
2004
Escherichia coli
Wen, Y.; Shi, X.; Yuan, Z.; Zhou, P.
Expression, purification, and characterization of His-tagged penicillin G acylase from Kluyvera citrophila in Escherichia coli
Protein Expr. Purif.
38
24-28
2004
Kluyvera cryocrescens
Cheng, T.; Chen, M.; Zheng, H.; Wang, J.; Yang, S.; Jiang, W.
Expression and purification of penicillin G acylase enzymes from four different micro-organisms, and a comparative evaluation of their synthesis/hydrolysis ratios for cephalexin
Protein Expr. Purif.
46
107-113
2006
Alcaligenes faecalis, Escherichia coli, Kluyvera cryocrescens, Providencia rettgeri
Wang, T.; Zhu, H.; Ma, X.; Ma, Y.; Wei, D.
Structure-based stabilization of an enzyme: the case of penicillin acylase from Alcaligenes faecalis
Protein Pept. Lett.
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177-183
2006
Alcaligenes faecalis
Pinotti, L.M.; Silva, A.F.; Silva, R.G.; Giordano, R.L.
Study of different media for production of penicillin G acylase from Bacillus megaterium ATCC 14945
Appl. Biochem. Biotechnol.
84-86
655-663
2000
Priestia megaterium
Pinotti, L.M.; Silva, R.G.; Giordano, R.C.; Giordano, R.L.
Inoculum studies in production of penicillin G acylase by Bacillus megaterium ATCC 14945
Appl. Biochem. Biotechnol.
98-100
679-686
2002
Priestia megaterium
Wang, J.; Zhang, Q.; Huang, H.; Yuan, Z.; Ding, D.; Yang, S.; Jiang, W.
Increasing synthetic performance of penicillin G acylase from Bacillus megaterium by site-directed mutagenesis
Appl. Microbiol. Biotechnol.
74
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2007
Priestia megaterium
Illanes, A.; Torres, R.; Cartagena, O.; Ruiz, A.
Evaluation of penicillin acylase production by two strains of Bacillus megaterium
Biol. Res.
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357-364
1993
Priestia megaterium
Acevedo, F.; Cooney, C.L.
Pencillin amidase production by Bacillus megaterium
Biotechnol. Bioeng.
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493-503
1973
Priestia megaterium
Zhang, L.F.; Li, Z.W.; Zhang, Q.J.
Cloning and expression of penicillin G acylase gene in Bacillus megaterium
Chin. J. Biotechnol.
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1991
Priestia megaterium, Priestia megaterium BM1
Martin, L.; Prieto, M.A.; Cortes, E.; Garcia, J.L.
Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945
FEMS Microbiol. Lett.
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1995
Priestia megaterium (Q60136)
Panbangred, W.; Weeradechapon, K.; Udomvaraphant, S.; Fujiyama, K.; Meevootisom, V.
High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant
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2000
Priestia megaterium
Chiang, C.; Bennett, R.E.
Purification and properties of penicillin amidase from Bacillus megaterium
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1967
Priestia megaterium, Priestia megaterium 14945
Kang, J.H.; Hwang, Y.; Yoo, O.J.
Expression of penicillin G acylase gene from Bacillus megaterium ATCC 14945 in Escherichia coli and Bacillus subtilis
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99-108
1991
Priestia megaterium
Yang, Y.; Biedendieck, R.; Wang, W.; Gamer, M.; Malten, M.; Jahn, D.; Deckwer, W.D.
High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium
Microb. Cell Fact.
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36
2006
Priestia megaterium
Basso, A.; Braiuca, P.; Cantone, S.; Ebert, C.; Linda, P.; Spizzo, P.; Caimi, P.; Hanefeld, U.; Degrassi, G.; Gardossi, L.
In silico analysis of enzyme surface and glycosylation effect as a tool for efficient covalent immobilisation of CalB and PGA on Sepabeads
Adv. Synth. Catal.
349
877-886
2007
Escherichia coli, Providencia rettgeri
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Montes, T.; Grazu, V.; Lopez-Gallego, F.; Hermoso, J.A.; Garcia, J.L.; Manso, I.; Galan, B.; Gonzalez, R.; Fernandez-Lafuente, R.; Guisan, J.M.
Genetic modification of the penicillin G acylase surface to improve its reversible immobilization on ionic exchangers
Appl. Environ. Microbiol.
73
312-319
2007
Escherichia coli
Torres-Bacete, J.; Hormigo, D.; Stuart, M.; Arroyo, M.; Torres, P.; Castillon, M.P.; Acebal, C.; Garcia, J.L.; de la Mata, I.
Newly discovered penicillin acylase activity of aculeacin A acylase from Actinoplanes utahensis
Appl. Environ. Microbiol.
73
5378-5381
2007
Actinoplanes utahensis
Wang, T.; Zhu, H.; Ma, X.; Fei, Z.; Ma, Y.; Wei, D.
Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene
Appl. Microbiol. Biotechnol.
72
953-958
2006
Alcaligenes faecalis
Grinberg, V.Y.; Burova, T.V.; Grinberg, N.V.; Shcherbakova, T.A.; Guranda, D.T.; Chilov, G.G.; Svedas, V.K.
Thermodynamic and kinetic stability of penicillin acylase from Escherichia coli
Biochim. Biophys. Acta
1784
736-746
2008
Escherichia coli
Fuentes, M.; Batalla, P.; Grazu, V.; Pessela, B.C.; Mateo, C.; Montes, T.; Hermoso, J.A.; Guisan, J.M.; Fernandez-Lafuente, R.
Mixed ion exchange supports as useful ion exchangers for protein purification: purification of penicillin G acylase from Escherichia coli
Biomacromolecules
8
703-707
2007
Escherichia coli
Zhang, W.G.; Wei, D.Z.; Yang, X.P.; Song, Q.X.
Penicillin acylase catalysis in the presence of ionic liquids
Bioprocess Biosyst. Eng.
29
379-383
2006
Escherichia coli
Koreishi, M.; Tani, K.; Ise, Y.; Imanaka, H.; Imamura, K.; Nakanishi, K.
Enzymatic synthesis of beta-lactam antibiotics and N-fatty-acylated amino compounds by the acyl-transfer reaction catalyzed by penicillin V acylase from Streptomyces mobaraensis
Biosci. Biotechnol. Biochem.
71
1582-1586
2007
Streptomyces mobaraensis
Pinotti, L.M.; Ribeiro de Souza, V.; de Campos Giordano, R.; de Lima Camargo Giordano, R.
The penicillin G acylase production by B. megaterium is amino acid consumption dependent
Biotechnol. Bioeng.
97
346-353
2007
Priestia megaterium
Polizzi, K.M.; Chaparro-Riggers, J.F.; Vazquez-Figueroa, E.; Bommarius, A.S.
Structure-guided consensus approach to create a more thermostable penicillin G acylase
Biotechnol. J.
1
531-536
2006
Escherichia coli (P06875)
Li, D.; Cheng, S.; Wei, D.; Ren, Y.; Zhang, D.
Production of enantiomerically pure (S)-beta-phenylalanine and (R)-beta-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium
Biotechnol. Lett.
29
1825-1830
2007
Escherichia coli
Cecchini, D.A.; Serra, I.; Ubiali, D.; Terreni, M.; Albertini, A.M.
New active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase
BMC Biotechnol.
7
54
2007
Escherichia coli
Jager, S.A.; Jekel, P.A.; Janssen, D.B.
Hybrid penicillin acylases with improved properties for synthesis of beta -lactam antibiotics
Enzyme Microb. Technol.
40
1335-1344
2007
Escherichia coli (P06875)
-
Kumar, R.S.; Prabhune, A.A.; Pundle, A.V.; Karthikeyan, M.; Suresh, C.G.
A tryptophan residue is identified in the substrate binding of penicillin G acylase from Kluyvera citrophila
Enzyme Microb. Technol.
40
1389-1397
2007
Kluyvera cryocrescens
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Cabrera, Z.; Lopez-Gallego, F.; Fernandez-Lorente, G.; Palomo, J.M.; Montes, T.; Grazu, V.; Guisan, J.M.; Fernandez-Lafuente, R.
Asymmetric hydrolysis of dimethyl phenylmalonate by immobilized penicillin G acylase from E. coli
Enzyme Microb. Technol.
40
997-1000
2007
Escherichia coli
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Cheng, S.; Song, Q.; Wei, D.; Gao, B.
High-level production penicillin G acylase from Alcaligenes faecalis in recombinant Escherichia coli with optimization of carbon sources
Enzyme Microb. Technol.
41
326-330
2007
Alcaligenes faecalis
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Chandel, A.K.; Rao, L.V.; Narasu, M.L.; Singh, O.V.
The realm of penicillin G acylase in beta-lactam antibiotics
Enzyme Microb. Technol.
42
199-207
2008
Priestia megaterium, Escherichia coli, Kluyvera cryocrescens
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Zhang, D.; Koreishi, M.; Imanaka, H.; Imamura, K.; Nakanishi, K.
Cloning and characterization of penicillin V acylase from Streptomyces mobaraensis
J. Biotechnol.
128
788-800
2007
Streptomyces mobaraensis (Q0R3W5), Streptomyces mobaraensis
Jager, S.A.; Shapovalova, I.V.; Jekel, P.A.; Alkema, W.B.; Svedas, V.K.; Janssen, D.B.
Saturation mutagenesis reveals the importance of residues alphaR145 and alphaF146 of penicillin acylase in the synthesis of beta-lactam antibiotics
J. Biotechnol.
133
18-26
2008
Escherichia coli
Rajendhran, J.; Gunasekaran, P.
Application of cross-linked enzyme aggregates of Bacillus badius penicillin G acylase for the production of 6-aminopenicillanic acid
Lett. Appl. Microbiol.
44
43-49
2007
Bacillus badius (Q45TR7), Bacillus badius
Carboni, C.; Kierkels, H.G.; Gardossi, L.; Tamiola, K.; Janssen, D.B.; Quaedflieg, P.J.
Preparation of D-amino acids by enzymatic kinetic resolution using a mutant of penicillin-G acylase from E. coli
Tetrahedron Asymmetry
17
245-251
2006
Escherichia coli
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Kumar, A.; Singh, S.; Poddar, P.; Prabhune, A.; Pundle, A.
Effect of cultural conditions and media constituents on production of penicillin V acylase and CTAB treatment to enhance whole-cell enzyme activity of Rhodotorula aurantiaca (NCIM 3425)
Appl. Biochem. Biotechnol.
157
463-472
2009
Buckleyzyma aurantiaca, Buckleyzyma aurantiaca NCIM 3425
Illanes, A.; Wilson, L.; Aguirre, C.
Synthesis of cephalexin in aqueous medium with carrier-bound and carrier-free penicillin acylase biocatalysts
Appl. Biochem. Biotechnol.
157
98-110
2009
Escherichia coli
Lambert, J.M.; Bongers, R.S.; de Vos, W.M.; Kleerebezem, M.
Functional analysis of four bile salt hydrolase and penicillin acylase family members in Lactobacillus plantarum WCFS1
Appl. Environ. Microbiol.
74
4719-4726
2008
Lactiplantibacillus plantarum
Chilov, G.G.; Stroganov, O.V.; Svedas, V.K.
Molecular modeling studies of substrate binding by penicillin acylase
Biochemistry (Moscow)
73
56-64
2008
Escherichia coli (P06875)
Bergeron, L.M.; Tokatlian, T.; Gomez, L.; Clark, D.S.
Redirecting the inactivation pathway of penicillin amidase and increasing amoxicillin production via a thermophilic molecular chaperone
Biotechnol. Bioeng.
102
417-424
2009
Escherichia coli
Romero, O.; Vergara, J.; Fernandez-Lafuente, R.; Guisan, J.M.; Illanes, A.; Wilson, L.
Simple strategy of reactivation of a partially inactivated penicillin g acylase biocatalyst in organic solvent and its impact on the synthesis of beta-lactam antibiotics
Biotechnol. Bioeng.
103
472-479
2009
Escherichia coli
Bernardino, S.M.; Fernandes, P.; Fonseca, L.P.
A new biocatalyst: Penicillin G acylase immobilized in sol-gel micro-particles with magnetic properties
Biotechnol. J.
4
695-702
2009
Escherichia coli
Massolini, G.; Temporini, C.; Calleri, E.
Penicillin G acylase as chiral selector in LC and CE: exploring the origins of enantioselectivity
J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.
875
20-29
2008
Rhizobium viscosum, Priestia megaterium, Escherichia coli, Kluyvera cryocrescens
Kafshnochi, M.; Farajnia, S.; Aboshof, R.; Babaei, H.; Aminolroayaee, M.
Cloning and over-expression of penicillin G acylase in Escherichia coli BL21
Afr. J. Biotechnol.
9
2697-2701
2010
Escherichia coli (D5M8S0)
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Tahir, A.; Aftab, M.; Mateen, B.; Jabeen, F.
Hyper production of enzyme penicillin amidase by locally isolated thermotolerant Bacillus sp. MARC-0103 from rice starch in cheese whey
Ann. Microbiol.
59
777-783
2009
Bacillus sp. (in: Bacteria)
Adikane, H.V.; Thakar, D.M.
Studies of penicillin G acylase immobilization using highly porous cellulose-based polymeric membrane
Appl. Biochem. Biotechnol.
160
1130-1145
2010
Escherichia coli, Escherichia coli NCIM 2400
Martinez-Hernandez, J.L.; Mata-Gomez, M.A.; Aguilar-Gonzalez, C.N.; Ilyina, A.
A process to produce penicillin G acylase by surface-adhesion fermentation using Mucor griseocyanus to obtain 6-aminopenicillanic acid by penicillin G hydrolysis
Appl. Biochem. Biotechnol.
160
2045-2053
2010
Mucor griseocyanus, Mucor griseocyanus H/55.1.1
Senerovic, L.; Stankovic, N.; Ljubijanic, G.; Vasiljevic, B.
Glycosylation and pH stability of penicillin G acylase from Providencia rettgeri produced in Pichia pastoris
Arch. Biol. Sci.
61
581-586
2009
Providencia rettgeri
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Hormigo, D.; De La Mata, I.; Castillon, M.; Acebal, C.; Arroyo, M.
Kinetic and microstructural characterization of immobilized penicillin acylase from Streptomyces lavendulae on Sepabeads EC-EP
Biocatal. Biotransform.
27
271-281
2009
Streptomyces lavendulae
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Pinotti, L.; Fonseca, L.; Prazeres, D.; Rodrigues, D.; Nucci, E.; Giordano, R.
Recovery and partial purification of penicillin G acylase from E. coli homogenate and B. megaterium culture medium using an expanded bed adsorption column
Biochem. Eng. J.
44
111-118
2009
Priestia megaterium, Escherichia coli, Priestia megaterium ATCC 14945, Escherichia coli ATCC 9637
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Xue, J.; Wang, A.; Zhou, C.; Shen, S.
Penicillin acylase immobilization depending on macromolecular crowding and catalysis in aqueous-organic medium
Bioprocess Biosyst. Eng.
32
765-772
2009
Priestia megaterium
Zhiryakova, D.; Guncheva, M.; Ivanov, I.; Stambolieva, N.
Hydrolysis of phenylacetanilides catalyzed by penicillin G acylase from Alcaligenes faecalis: sensitivity of the reaction to substitution in the leaving group
Catal. Commun.
11
196-201
2009
Alcaligenes faecalis
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Krzeslak, J.; Braun, P.; Voulhoux, R.; Cool, R.H.; Quax, W.J.
Heterologous production of Escherichia coli penicillin G acylase in Pseudomonas aeruginosa
J. Biotechnol.
142
250-258
2009
Escherichia coli
Shi, Y.F.; Soumillion, P.; Ueda, M.
Effects of catalytic site mutations on active expression of phage fused penicillin acylase
J. Biotechnol.
145
139-142
2010
Escherichia coli
Pchelintsev, N.; Youshko, M.; Švedas, V.
Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase
J. Mol. Catal. B
56
202-207
2009
Escherichia coli
-
Kumar, A.; Pundle, A.
Effect of organic solvents on cell-bound penicillin V acylase activity of Erwinia aroideae (DSMZ 30186): A permeabilization effect
J. Mol. Catal. B
57
67-71
2009
Pectobacterium aroidearum
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Kumar, A.; Gowda, N.; Gaikwad, S.; Pundle, A.
Rhodotorula aurantiaca penicillin V acylase: active site characterization and fluorometric studies
J. Photochem. Photobiol. B
97
109-116
2009
Buckleyzyma aurantiaca
Wilson, L.; Illanes, A.; Soler, L.; Henriquez, M.
Effect of the degree of cross-linking on the properties of different CLEAs of penicillin acylase
Process Biochem.
44
322-326
2009
Escherichia coli
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Yuryev, R.; Kasche, V.; Ignatova, Z.; Galunsky, B.
Improved A. faecalis penicillin amidase mutant retains the thermodynamic and pH stability of the wild type enzyme
Protein J.
29
181-187
2010
Alcaligenes faecalis
Cecchini, D.A.; Pavesi, R.; Sanna, S.; Daly, S.; Xaiz, R.; Pregnolato, M.; Terreni, M.
Efficient biocatalyst for large-scale synthesis of cephalosporins, obtained by combining immobilization and site-directed mutagenesis of penicillin acylase
Appl. Microbiol. Biotechnol.
95
1491-1500
2012
Escherichia coli (P06875), Escherichia coli
Blum, J.K.; Deaguero, A.L.; Perez, C.V.; Bommarius, A.S.
Ampicillin synthesis using a two-enzyme cascade with both alpha-amino ester hydrolase and penicillin G acylase
ChemCatChem
2
987-991
2010
Escherichia coli
Rathinaswamy, P.; Gaikwad, S.M.; Suresh, C.G.; Prabhune, A.A.; Brannigan, J.A.; Dodson, G.G.; Pundle, A.V.
Purification and characterization of YxeI, a penicillin acylase from Bacillus subtilis
Int. J. Biol. Macromol.
50
25-30
2012
Bacillus subtilis (Q2HPP6), Bacillus subtilis
Miranda, V.; Wilson, L.; Cardenas, C.; Illanes, A.
Reactivation of immobilized penicillin G acylase: Influence of cosolvents and catalytic modulators
J. Mol. Catal. B
68
77-82
2011
Escherichia coli
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Deaguero, A.; Blum, J.; Bommarius, A.
Improving the diastereoselectivity of penicillin G acylase for ampicillin synthesis from racemic substrates
Protein Eng. Des. Sel.
25
135-144
2012
Escherichia coli (P06875)
Gong, X.; Su, E.; Wang, P.; Wei, D.
Alcaligenes faecalis penicillin G acylase-catalyzed enantioselective acylation of dl-phenylalanine and derivatives in aqueous medium
Tetrahedron Lett.
52
5398-5402
2011
Alcaligenes faecalis
-
Varshney, N.K.; Kumar, R.S.; Ignatova, Z.; Prabhune, A.; Pundle, A.; Dodson, E.; Suresh, C.G.
Crystallization and X-ray structure analysis of a thermostable penicillin G acylase from Alcaligenes faecalis
Acta Crystallogr. Sect. F
68
273-277
2012
Alcaligenes faecalis (O34142), Alcaligenes faecalis
Varshney, N.K.; Ramasamy, S.; Brannigan, J.A.; Wilkinson, A.J.; Suresh, C.G.
Cloning, overexpression, crystallization and preliminary X-ray crystallographic analysis of a slow-processing mutant of penicillin G acylase from Kluyvera citrophila
Acta Crystallogr. Sect. F
69
925-929
2013
Kluyvera cryocrescens (P07941), Kluyvera cryocrescens, Kluyvera cryocrescens DMSZ 2660 (P07941)
Torres, L.L.; Cantero, A.; del Valle, M.; Marina, A.; Lopez-Gallego, F.; Guisan, J.M.; Berenguer, J.; Hidalgo, A.
Engineering the substrate specificity of a thermophilic penicillin acylase from Thermus thermophilus
Appl. Environ. Microbiol.
79
1555-1562
2013
Thermus thermophilus (Q72G76), Thermus thermophilus
Zhang, Q.; Xu, H.; Zhao, J.; Zeng, R.
Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library
Biotechnol. Lett.
36
617-625
2014
uncultured bacterium (D6R1Q1)
Orr, V.; Scharer, J.; Moo-Young, M.; Honeyman, C.; Fenner, D.; Crossley, L.; Suen, S.; Chou, C.
Integrated development of an effective bioprocess for extracellular production of penicillin G acylase in Escherichia coli and its subsequent one-step purification
J. Biotechnol.
161
19-26
2012
Escherichia coli, Escherichia coli JE5505
Moenster, A.; Villain, L.; Scheper, T.; Beutel, S.
One-step-purification of penicillin G amidase from cell lysate using ion-exchange membrane adsorbers
J. Membr. Sci.
444
359-364
2013
Escherichia coli
-
Torres-Bacete, J.; Hormigo, D.; Torres-Guzman, R.; Arroyo, M.; Castillon, M.; Garcia, J.; Acebal, C.; de la Mata, I.
Overexpression of penicillin V acylase from Streptomyces lavendulae and elucidation of its catalytic residues
Appl. Environ. Microbiol.
81
1225-1233
2015
Streptomyces lavendulae subsp. lavendulae (Q539C0), Streptomyces lavendulae subsp. lavendulae ATCC 13664 (Q539C0)
Adediran, S.A.; Pratt, R.F.
Penicillin acylase and O-aryloxycarbonyl hydroxamates Two acyl-enzymes, one leading to hydrolysis, the other to inactivation
Arch. Biochem. Biophys.
614
65-71
2017
Escherichia coli
Avinash, V.; Pundle, A.; Ramasamy, S.; Suresh, C.
Penicillin acylases revisited Importance beyond their industrial utility
Crit. Rev. Biotechnol.
36
303-316
2016
Achromobacter sp., Alcaligenes faecalis, Achromobacter xylosoxidans, Bacillus badius, Bacillus subtilis, Lysinibacillus sphaericus, Fusarium oxysporum, Providencia rettgeri, Escherichia coli (P06875), Kluyvera cryocrescens (P07941), Rhizobium viscosum (P31956), Priestia megaterium (Q60136)
Xu, G.; Zhao, Q.; Huang, B.; Zhou, J.; Cao, F.
Directed evolution of a penicillin V acylase from Bacillus sphaericus to improve its catalytic efficiency for 6-APA production
Enzyme Microb. Technol.
119
65-70
2018
Lysinibacillus sphaericus (P12256), Lysinibacillus sphaericus
Ye, J.; Li, A.; Chu, T.; Pan, X.; He, B.
Poly-lysine supported cross-linked enzyme aggregates of penicillin G acylase and its application in synthesis of beta-lactam antibiotics
Int. J. Biol. Macromol.
140
423-428
2019
Providencia rettgeri (A0A2I6UI54), Providencia rettgeri, Providencia rettgeri PX04 (A0A2I6UI54)
Avinash, V.S.; Ramasamy, S.; Suresh, C.G.; Pundle, A.
Penicillin V acylase from Pectobacterium atrosepticum exhibits high specific activity and unique kinetics
Int. J. Biol. Macromol.
79
1-7
2015
Pectobacterium atrosepticum (Q6D291), Pectobacterium atrosepticum, Pectobacterium atrosepticum ATCC BAA-672 (Q6D291)
Grulich, M.; Brezovsky, J.; Stepanek, V.; Palyzova, A.; Kyslikova, E.; Kyslik, P.
Resolution of alpha/beta-amino acids by enantioselective penicillin G acylase from Achromobacter sp.
J. Mol. Catal. B
122
240-247
2015
Escherichia coli (P06875), Achromobacter sp. CCM 4824 (Q3ZEF0)
-
Avinash, V.S.; Panigrahi, P.; Chand, D.; Pundle, A.; Suresh, C.G.; Ramasamy, S.
Structural analysis of a penicillin V acylase from Pectobacterium atrosepticum confirms the importance of two Trp residues for activity and specificity
J. Struct. Biol.
193
85-94
2016
Pectobacterium atrosepticum (Q6D291), Pectobacterium atrosepticum, Pectobacterium atrosepticum ATCC BAA-672 (Q6D291)
Ayakar, S.; Yadav, G.
Development of novel support for penicillin acylase and its application in 6-aminopenicillanic acid production
Mol. Catal.
476
110484
2019
Escherichia coli (P06875)
-
Philem, P.D.; Sonalkar, V.V.; Dharne, M.S.; Prabhune, A.A.
Purification and partial characterization of novel penicillin V acylase from Acinetobacter sp. AP24 isolated from Loktak Lake, an Indo-Burma biodiversity hotspot
Prep. Biochem. Biotechnol.
46
524-530
2016
Acinetobacter sp. AP-24
Chand, D.; Varshney, N.; Ramasamy, S.; Panigrahi, P.; Brannigan, J.A.; Wilkinson, A.J.; Suresh, C.G.
Structure mediation in substrate binding and post-translational processing of penicillin acylases Information from mutant structures of Kluyvera citrophila penicillin G acylase
Protein Sci.
24
1660-1670
2015
Kluyvera cryocrescens (P07941), Kluyvera cryocrescens
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