Information on EC 3.5.1.101 - L-proline amide hydrolase

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The expected taxonomic range for this enzyme is: Pseudomonas azotoformans

EC NUMBER
COMMENTARY hide
3.5.1.101
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RECOMMENDED NAME
GeneOntology No.
L-proline amide hydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-piperidine-2-carboxamide + H2O = (S)-piperidine-2-carboxylate + NH3
show the reaction diagram
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L-prolinamide + H2O = L-proline + NH3
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
(S)-piperidine-2-carboxamide amidohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
79633-25-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
IAM 1603
SwissProt
Manually annotated by BRENDA team
IAM 1603
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R,S)-piperazine-2-tert-butylcarboxamide + H2O
(S)-piperazine-2-tert-butylcarboxylic acid + NH3
show the reaction diagram
(S)-piperazine-2-carboxamide + H2O
(S)-piperazine-2-carboxylic acid + NH3
show the reaction diagram
(S)-piperazine-2-tert-butylcarboxamide + H2O
(S)-piperazine-2-carboxylic acid + tert-butylamine
show the reaction diagram
0.2% of the activity with L-proline amide
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?
(S)-piperidine-2-carboxamide + H2O
(S)-piperidine-2-carboxylic acid + NH3
show the reaction diagram
32% of the activity with L-proline amide
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?
L-alanine amide + H2O
L-alanine + NH3
show the reaction diagram
10.6% of the activity with L-proline amide
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?
L-isoleucine amide + H2O
L-isoleucine + NH3
show the reaction diagram
0.17% of the activity with L-proline amide
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?
L-leucine amide + H2O
L-leucine + NH3
show the reaction diagram
L-methionine amide + H2O
L-methionine + NH3
show the reaction diagram
4.2% of the activity with L-proline amide
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?
L-phenylalanine amide + H2O
L-phenylalanine + NH3
show the reaction diagram
L-proline amide + H2O
L-proline + NH3
show the reaction diagram
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-
-
?
L-proline-p-nitroanilide + H2O
? + NH3
show the reaction diagram
40.9% of the activity with L-proline amide
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?
L-serine amide + H2O
L-serine + NH3
show the reaction diagram
0.43% of the activity with L-proline amide
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?
L-threonine amide + H2O
L-threonine + NH3
show the reaction diagram
0.12% of the activity with L-proline amide
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?
L-tryptophan amide + H2O
L-tryptophan + NH3
show the reaction diagram
0.2% of the activity with L-proline amide
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?
L-tyrosine amide + H2O
L-tyrosine + NH3
show the reaction diagram
0.086% of the activity with L-proline amide
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?
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
1 mM, complete inhibition
Cd2+
1 mM, complete inhibition
CoCl2
1 mM, 52% inhibition
Hg2+
1 mM, complete inhibition
iodoacetate
1 mM, 40% inhibition
NEM
1 mM, 24% inhibition
NiCl2
1 mM, 70% inhibition
p-chloromercuribenzoate
1 mM, 67% inhibition
PbCl2
1 mM, 73% inhibition
phenylhydrazine
1 mM, complete inhibition
Zn2+
1 mM, complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.58
L-proline-p-nitroanilide
30°C, pH 7.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000894
S-stereoselective amidase from Pseudomonas azotoformans IAM 1603
192
LaaA from Escharichia coli JM109 harboring pSTB20
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stored without loss of activity for more than six months in the buffer containing 50% glycerol
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis