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Information on EC 3.4.24.B6 - matrix metalloproteinase-20 and Organism(s) Mus musculus and UniProt Accession P57748

for references in articles please use BRENDA:EC3.4.24.B6
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.B6 matrix metalloproteinase-20
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This record set is specific for:
Mus musculus
UNIPROT: P57748
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Tetrapoda
Reaction Schemes
proteolytic degradation of ameloblastin
proteolytic cleavage of ameloblastin
Synonyms
mmp20, mmp-20, enamelysin, matrix metalloproteinase-20, matrix metalloproteinase 20, enamel protease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
enamel metalloproteinase
-
M10.019
Merops-ID
enamelysin
matrix metalloproteinase 20
-
-
MMP-20
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
CAS REGISTRY NUMBER
COMMENTARY hide
185766-51-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
proteins + H2O
peptides
show the reaction diagram
-
-
?
amelogenin + H2O
?
show the reaction diagram
casein + H2O
?
show the reaction diagram
-
zymography
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
proteins + H2O
peptides
show the reaction diagram
-
-
?
amelogenin + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
marimastat
-
alters morphogenesis and mineralization of the tooth germs associated with inhibition of activation of MMP-20
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at room temperature
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
specialized cell that deposits tooth enamel
Manually annotated by BRENDA team
minute quantities, 5000fold lower than in 4-day-old first molar tooth buds
Manually annotated by BRENDA team
-
from mouse embryos at E18.5 and postnatal day 3 mice
Manually annotated by BRENDA team
only in jaw containing the developing tooth buds appreciable levels of the enzyme
Manually annotated by BRENDA team
-
from hemimandibles and hemimaxillae
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
MMP-20 is synthesized and secreted by ameloblasts
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
malfunction
-
Mmp20-deficient mouse ameloblasts can initiate, terminate, and reinitiate the secretory stage of enamel development
metabolism
-
TGF-beta signaling involved in amelogenesis is partially mediated by regulating the expression of MMP20 mRNA
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MMP20_MOUSE
482
0
54373
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21000
-
Western blot analysis, active form of MMP-20
54000
-
Western blot analysis
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
enamel from MMP-20 null mice is ca. 37% softer, contains 53% less bulk mineral and has 7-16% higher levels of water and protein per unit weight than wild type enamel
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Q-Sepharose column chromatography and heparin Sepharose 6 column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
isolation, characterization, and chromosomal location mapping of the mouse enamelysin gene locus
real-time PCR MMP-20 expression analysis, overview
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Mmp20 expression levels must be within a specific range for normal enamel development to occur. Creation of a normally thick enamel layer may occur over a wider range of Mmp20 expression levels, but acquisition of normal enamel hardness has a narrower range
increased expression of ODAM and Runx2 augments MMP-20 expression, and both TGF-beta1 and BMP-2 up-regulate Runx2 expression. TGF-beta1 promotes MMP-20 expression in ameloblasts
-
loss and siRNA-mediated silencing of Runx2 in ameloblast-lineage cells decreases ODAM expression, resulting in downregulation of MMP-20 expression
-
transforming growth factor-beta1, TGF-beta1, prominently induces MMP20 expression in ameloblast-lineage cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
critical role of MMPs in the processing and maturation of the dental matrix
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Mus musculus (P57748), Sus scrofa (P79287)
Manually annotated by BRENDA team
Turk, B.E.; Lee, D.H.; Yamakoshi, Y.; Klingenhoff, A.; Reichenberger, E.; Wright, J.T.; Simmer, J.P.; Komisarof, J.A.; Cantley, L.C.; Bartlett, J.D.
MMP-20 is predominately a tooth-specific enzyme with a deep catalytic pocket that hydrolyzes type V collagen
Biochemistry
45
3863-3874
2006
Homo sapiens, Mus musculus (Q3LRH7), Mus musculus
Manually annotated by BRENDA team
Bourd-Boittin, K.; Fridman, R.; Fanchon, S.; Septier, D.; Goldberg, M.; Menashi, S.
Matrix metalloproteinase inhibition impairs the processing, formation and mineralization of dental tissues during mouse molar development
Exp. Cell Res.
304
493-505
2005
Mus musculus
Manually annotated by BRENDA team
Bartlett, J.D.; Beniash, E.; Lee, D.H.; Smith, C.E.
Decreased mineral content in MMP-20 null mouse enamel is prominent during the maturation stage
J. Dent. Res.
83
909-913
2004
Mus musculus, Mus musculus C57BL6
Manually annotated by BRENDA team
Bartlett, J.D.; Skobe, Z.; Lee, D.H.; Wright, J.T.; Li, Y.; Kulkarni, A.B.; Gibson, C.W.
A developmental comparison of matrix metalloproteinase-20 and amelogenin null mouse enamel
Eur. J. Oral Sci.
114
18-23
2006
Mus musculus
Manually annotated by BRENDA team
Caterina, J.; Shi, J.; Krakora, S.; Bartlett, J.D.; Engler, J.A.; Kozak, C.A.; Birkedal-Hansen, H.
Isolation, characterization, and chromosomal location of the mouse enamelysin gene
Genomics
62
308-111
1999
Mus musculus (P57748), Mus musculus
Manually annotated by BRENDA team
Caterina, J.; Shi, J.; Sun, X.; Qian, Q.; Yamada, S.; Liu, Y.; Krakora, S.; Bartlett, J.D.; Yamada, Y.; Engler, J.A.; Birkedal-Hansen, H.; Simmer, J.P.
Cloning, characterization, and expression analysis of mouse enamelysin
J. Dent. Res.
79
1697-1703
2000
Mus musculus (P57748), Mus musculus
Manually annotated by BRENDA team
Gao, Y.; Li, D.; Han, T.; Sun, Y.; Zhang, J.
TGF-beta1 and TGFBR1 are expressed in ameloblasts and promote MMP20 expression
Anat. Rec. (Hoboken)
292
885-890
2009
Mus musculus
Manually annotated by BRENDA team
Lee, H.K.; Lee, D.S.; Ryoo, H.M.; Park, J.T.; Park, S.J.; Bae, H.S.; Cho, M.I.; Park, J.C.
The odontogenic ameloblast-associated protein (ODAM) cooperates with RUNX2 and modulates enamel mineralization via regulation of MMP-20
J. Cell. Biochem.
111
755-767
2010
Mus musculus
Manually annotated by BRENDA team
Bartlett, J.D.; Yamakoshi, Y.; Simmer, J.P.; Nanci, A.; Smith, C.E.
MMP20 cleaves E-cadherin and influences ameloblast development
Cells Tissues Organs
194
222-226
2011
Mus musculus, Sus scrofa
Manually annotated by BRENDA team
Shin, M.; Hu, Y.; Tye, C.E.; Guan, X.; Deagle, C.C.; Antone, J.V.; Smith, C.E.; Simmer, J.P.; Bartlett, J.D.
Matrix metalloproteinase-20 over-expression is detrimental to enamel development: a Mus musculus model
PLoS ONE
9
e86774
2014
Mus musculus (P57748), Mus musculus
Manually annotated by BRENDA team
Prajapati, S.; Tao, J.; Ruan, Q.; De Yoreo, J.J.; Moradian-Oldak, J.
Matrix metalloproteinase-20 mediates dental enamel biomineralization by preventing protein occlusion inside apatite crystals
Biomaterials
75
260-270
2016
Mus musculus (P57748)
Manually annotated by BRENDA team
Yamazaki, H.; Tran, B.; Beniash, E.; Kwak, S.Y.; Margolis, H.C.
Proteolysis by MMP20 prevents aberrant mineralization in secretory enamel
J. Dent. Res.
98
468-475
2019
Mus musculus (P57748)
Manually annotated by BRENDA team
Shin, M.; Suzuki, M.; Guan, X.; Smith, C.; Bartlett, J.
Murine matrix metalloproteinase-20 overexpression stimulates cell invasion into the enamel layer via enhanced Wnt signaling
Sci. Rep.
6
29492
2016
Mus musculus (P57748), Mus musculus
Manually annotated by BRENDA team