Information on EC 3.4.24.B30 - aeropyrolysin

for references in articles please use BRENDA:EC3.4.24.B30
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The expected taxonomic range for this enzyme is: Archaea, Bacteria

EC NUMBER
COMMENTARY hide
3.4.24.B30
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
aeropyrolysin
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-methoxycoumarin-4-acetyl-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2 + H2O
?
show the reaction diagram
7-methoxycoumarin-4-acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O
?
show the reaction diagram
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Leu-A2pr(Dnp)-Ala-Arg-NH2 + H2O
?
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
the maximal enzymatic activity is obtained at 110C in the presence of CaCl2. In the absence of CaCl2, the maximal activity observed is obtained at 100C. Below 100C the enzyme activity does not show significant differences either with or without CaCl2. Concentrations higher than 1 mM CaCl2 show an inhibitory effect on enzymatic activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
-
the maximal enzymatic activity is obtained at 110C in the presence of CaCl2. In the absence of CaCl2, the maximal activity observed is obtained at 100C. Below 100C the enzyme activity does not show significant differences either with or without CaCl2. Concentrations higher than 1 mM CaCl2 show an inhibitory effect on enzymatic activity
diisopropyl fluorophosphate
-
1 mM, 81% inhibition
EDTA
-
1 mM, complete loss of activity
EGTA
-
1 mM, 96% inhibition
phenylmethylsulfonyl fluoride
-
1 mM, 45% inhibition
soybean trypsin
-
1 mg/ml, 82% inhibition
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SDS
-
1%, 7% loss of acivity. 2% 2.6fold activation
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
azocasein hydrolysis
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 9
-
pH 4.5: about 30% of maximal activity, pH 9.0: about 65% loss of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
in absence of CaCl2
110
-
in the presence of 1 mM CaCl2
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80 - 130
-
80C: about 50% of maximal activity, 130C: about 35% of maximal activity, in the presence of 1 mM CaCl2
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
-
1 * 52000, SDS-PAGE
53000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120
-
half-life: 2.5 h, in the presence of 1 mM CaCl2
125
-
half-life: 1.2 h, in the presence of 1 mM CaCl2
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-mercaptoethanol
dithiothreitol
guanidine-HCl
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE