Information on EC 3.4.24.B30 - aeropyrolysin

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The expected taxonomic range for this enzyme is: Aeropyrum pernix

EC NUMBER
COMMENTARY hide
3.4.24.B30
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
aeropyrolysin
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-methoxycoumarin-4-acetyl-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(Dnp)-NH2 + H2O
?
show the reaction diagram
7-methoxycoumarin-4-acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O
?
show the reaction diagram
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Leu-A2pr(Dnp)-Ala-Arg-NH2 + H2O
?
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
the maximal enzymatic activity is obtained at 110°C in the presence of CaCl2. In the absence of CaCl2, the maximal activity observed is obtained at 100°C. Below 100°C the enzyme activity does not show significant differences either with or without CaCl2. Concentrations higher than 1 mM CaCl2 show an inhibitory effect on enzymatic activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
-
the maximal enzymatic activity is obtained at 110°C in the presence of CaCl2. In the absence of CaCl2, the maximal activity observed is obtained at 100°C. Below 100°C the enzyme activity does not show significant differences either with or without CaCl2. Concentrations higher than 1 mM CaCl2 show an inhibitory effect on enzymatic activity
diisopropyl fluorophosphate
-
1 mM, 81% inhibition
EDTA
-
1 mM, complete loss of activity
EGTA
-
1 mM, 96% inhibition
phenylmethylsulfonyl fluoride
-
1 mM, 45% inhibition
soybean trypsin
-
1 mg/ml, 82% inhibition
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SDS
-
1%, 7% loss of acivity. 2% 2.6fold activation
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
azocasein hydrolysis
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 9
-
pH 4.5: about 30% of maximal activity, pH 9.0: about 65% loss of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
in absence of CaCl2
110
-
in the presence of 1 mM CaCl2
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80 - 130
-
80°C: about 50% of maximal activity, 130°C: about 35% of maximal activity, in the presence of 1 mM CaCl2
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
-
1 * 52000, SDS-PAGE
53000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120
-
half-life: 2.5 h, in the presence of 1 mM CaCl2
125
-
half-life: 1.2 h, in the presence of 1 mM CaCl2
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-mercaptoethanol
dithiothreitol
guanidine-HCl
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE