Information on EC 3.4.24.B28 - ADAM15

Word Map on EC 3.4.24.B28
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.24.B28
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
ADAM15
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
proteolysis of proteins
show the reaction diagram
-
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
rheumatoid arthritis synovial fibroblasts display significantly higher caspase 3/7 activity upon camptothecin and FasL exposure when ADAM15 is down-regulated by specific small interfering RNAs. There is also a marked reduction of apoptosis upon knockdown of ADAM15 protein expression
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
major histocompatibility complex class I polypeptide-related sequence B + H2O
?
show the reaction diagram
-
ectodomain shedding
-
-
?
nephrocystin + H2O
?
show the reaction diagram
additional information
?
-
-
in contrast other ADAM15 isoforms ADAM15i3 interacts preferentially with Hck and Lyn containing proteins
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
major histocompatibility complex class I polypeptide-related sequence B + H2O
?
show the reaction diagram
-
ectodomain shedding
-
-
?
nephrocystin + H2O
?
show the reaction diagram
additional information
?
-
-
in contrast other ADAM15 isoforms ADAM15i3 interacts preferentially with Hck and Lyn containing proteins
-
-
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
x * 45000, mature enzyme, SDS-PAGE
110000
x * 110000, precursor, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Blue Sepharose column chromatography, nickel chelate column chromatography, and DEAE anion exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as a GST-fusion protein in Escherichia coli
-
the disintegrin domain of ADAM15 fused to human serum albumin is expressed in Pichia pastoris strain GS115
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
ADAM15 expression is down-regulated in melanoma metastasis compared to primary melanoma. Interferon-gamma and tumor growth factor-beta1 down-regulate ADAM15 protein levels in melanoma cells
-
enzyme expression is increased in pancreatic cancer cells compared to normal pancreatic tissue
-
severe hypoxia persistently up-regulates ADAM15 mRNA and protein levels in hippocampus and neocortex but not in thalamus or hypothalamus
-
the enzyme is up-regulated in the rheumatoid arthritis synovial membrane
-