Information on EC 3.4.24.B26 - myroilysin

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The expected taxonomic range for this enzyme is: Myroides profundi

EC NUMBER
COMMENTARY hide
3.4.24.B26
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
myroilysin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
broad specificity, elastinolytic activity and collagen-swelling ability
show the reaction diagram
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-
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
bovine elastin + H2O
?
show the reaction diagram
casein + H2O
?
show the reaction diagram
Collagen + H2O
?
show the reaction diagram
Elastin + H2O
?
show the reaction diagram
the enzyme shows much higher elastinolytic activity than the bacterial elastinase pseudolysin (EC 3.4.24.26). It has 6.78 U/mg elastinolytic activity in artificial seawater at 4°C, suggesting that it probably can degrade elastin in the deep sea
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-
?
elastinorcein + H2O
?
show the reaction diagram
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-
-
?
Fibrin + H2O
?
show the reaction diagram
hydrolyzes alpha, beta and gamma fibrin. Cleavage sites on fibrinogen and fibrin are different from those of pseudolysin
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-
?
Fibrinogen + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
-
-
-
?
Insulin B-chain + H2O
?
show the reaction diagram
N-succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Ala + p-nitroaniline
show the reaction diagram
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
show the reaction diagram
-
-
-
?
N-succinyl-Phe-Ala-Ala-Phe-p-nitroanilide + H2O
N-succinyl-Phe-Ala-Ala-Phe + p-nitroaniline
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Collagen + H2O
?
show the reaction diagram
Elastin + H2O
?
show the reaction diagram
B5B0E6
the enzyme shows much higher elastinolytic activity than the bacterial elastinase pseudolysin (EC 3.4.24.26). It has 6.78 U/mg elastinolytic activity in artificial seawater at 4°C, suggesting that it probably can degrade elastin in the deep sea
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
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2 mM NaCl increases enzyme binding to the elastin fibres
Zn
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the enzyme has the same conserved residues at the four zinc ligands as astacin (EC 3.4.24.21)
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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4 mM, complete inhibition
Co2+
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inhibits the caseinolytic and elastinolytic activities
EDTA
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moderate inhibitory effect
EGTA
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moderate inhibitory effect
Fe2+
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inhibits the caseinolytic and elastinolytic activities
Ni2+
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inhibits the caseinolytic and elastinolytic activities
Sr2+
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inhibits the caseinolytic and elastinolytic activities
Triton X-100
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significantly decreases enzyme binding to elastin
Tween 20
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significantly decreases enzyme binding to elastin
Tween 60
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significantly decreases enzyme binding to elastin
additional information
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Li+, K+, Mg2+, Ca2+, Sr2+, Ba2+, and Na+ have a little or no effect on activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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Km-value for casein at 50°C is 0.38%
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
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at 40°C, substrate: elastinorcein
9.5
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at 50°C; substrate: casein
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 45
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pH 9.0, substrate: elastinorcein
50
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pH 9.0, substrate: casein
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3
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calculated from sequence
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22936
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1 * 22936, mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
half-life: 28 min
43
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apparent melting temperature is 42.6°C
45
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half-life: 5 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE