Information on EC 3.4.24.B24 - L-alanine-D-glutamate endopeptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.24.B24
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
L-alanine-D-glutamate endopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endohydrolysis of the linkage of L-alanine-D-glutamic acid in the stem of a peptidoglycan
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
Listeria bacteriophage
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
peptidoglycan hydrolysis
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
the enzymatically active domain, EAD, is linked to a cell-wall-binding domain, CBD
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17600
h-deltaCwlK, determined by SDS-PAGE and zymography
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the crystal structure of the enzymatically active domain of Ply500 is solved at 1.8 A resolution
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified on a nickel column
using a streptavidin and a Superdex-75 gel-filtration column
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
into the pASK-IBA5 vector for expression of truncated Ply500 fragments, EAD500(1-141), EAD500(1-151), EAD500(1-163), in Escherichia coli JM83 cells
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truncated CwlK fused with a histidine-tag, h-deltaCwlK, is produced in Escherichia coli cells, fusions with the 3xFLAG-tag are constructed
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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bacteriophage peptidoglycan-hydrolyzing enzymes have found several applications in biotechnology and medicine, e.g. for surface-decontamination purposes, for biopreservation of food and feed and as potential topical antimicrobials