Information on EC 3.4.24.B2 - oligoendopeptidase F

Word Map on EC 3.4.24.B2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.4.24.B2
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
oligoendopeptidase F
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
proteolytic cleavage of proteins at Leu-Gly
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
110639-28-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 23-7A
SwissProt
Manually annotated by BRENDA team
strain 23-7A
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain, LS2195, aquatic bacterium
-
-
Manually annotated by BRENDA team
strain, LS2195, aquatic bacterium
-
-
Manually annotated by BRENDA team
formerly Geobacillus lituanicus DSM 15325, gene GT-SM3B
UniProt
Manually annotated by BRENDA team
formerly Geobacillus lituanicus DSM 15325, gene GT-SM3B
UniProt
Manually annotated by BRENDA team
strain NRC-1
-
-
Manually annotated by BRENDA team
strain NRC-1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-phenylazobenzyloxycarbonyl-L-Pro-Leu-Gly-L-Pro-D-Arg + H2O
?
show the reaction diagram
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) + H2O
?
show the reaction diagram
-
-
-
-
?
adrenocorticotropic hormone fragment 1-10 + H2O
SYSMEHF + RWG
show the reaction diagram
-
-
?
adrenocorticotropic hormone fragment 1-14 + H2O
SYSMEHF + RWG + KPVG
show the reaction diagram
-
-
?
adrenocorticotropic hormone fragment 1-17 + H2O
SYSMEHF + RWG + KPV + GKKR
show the reaction diagram
-
-
?
adrenocorticotropic hormone fragment 4-10 + H2O
MEHF + RWG
show the reaction diagram
-
-
?
beta-casomorphin + H2O
YPFP + GPI
show the reaction diagram
-
-
?
Bradykinin + H2O
?
show the reaction diagram
-
best substrate among peptides
-
-
?
bradykinin + H2O
RPPGF + SPR
show the reaction diagram
best substrate
-
?
carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala + H2O
?
show the reaction diagram
DesPro2bradykinin + H2O
RPGF + SPFR
show the reaction diagram
-
-
?
insulin chain B fragment 22-30 + H2O
RGFF + Tyr + TPKA
show the reaction diagram
-
-
?
Lys1bradykinin + H2O
KPPGF + SPFR
show the reaction diagram
-
-
?
N-(3-(2-furyl)acryloyl)-Leu-Gly-Pro-Ala + H2O
?
show the reaction diagram
N-carboxylbenzoyl-Gly-Pro-Leu-Gly-Pro + H2O
?
show the reaction diagram
neurotensin + H2O
Pyr-LYENKPR + RPYIL
show the reaction diagram
-
-
?
protein + H2O
peptides
show the reaction diagram
substance P fragment 2-11 + H2O
PKPQQ + Phe + Phe + GLM-NH2
show the reaction diagram
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
protein + H2O
peptides
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
increases activity
Li+
5% activation at 1 mM
Na+
27% activation at 1 mM
additional information
no effect by 1 mM K+ and 1-5 mM Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2-mercaptoethanol
14% inhibition at 1 mM
Brij 35
complete inhibition at 1 mM
Brij 58
complete inhibition at 1 mM
Cd2+
inhibits at 10 mM
Co2+
6% inhibition at 1 mM
dithiothreitol
-
-
DTT
42% inhibition at 1 mM
ethylene glycol
34% inhibition at 1%
Fe2+
inhibits at 10 mM
Fe3+
77% inhibition at 1 mM
glycerol
26% inhibition at 5%, 15% ativation at 1%
Guanidine-HCl
22% inhibition at 1 mM
Mn2+
51% inhibition at 1 mM
NaCl
-
0.001 mM decreases enzyme activity by 25%
Ni2+
41% inhibition at 1 mM
Pb2+
inhibits at 10 mM
SDS
48% inhibition at 1 mM, complete inhibition at 10 mM
Triton X-100
37% inhibition at 1%
Triton X-305
98% inhibition at 1%
-
Tween 20
33% inhibition at 0.1%
Tween 40
42% inhibition at 0.1%
Tween 60
38% inhibition at 0.1%
Tween 80
58% inhibition at 0.1%
Zn2+
14% inhibition at 1 mM
additional information
not inhibited by E-64, iodoacetamide, phosporamidon and pehylmethylsulfonyl fluoride
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycerol
26% inhibition at 5%, 15% ativation at 1%
TCEP
10% activation at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00217
Carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala
pH 7.3, 40°C, recombinant wild-type enzyme
0.0992
N-(3-(2-furyl)acryloyl)-Leu-Gly-Pro-Ala
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.99
Carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala
Geobacillus thermoleovorans
W5RWH8
pH 7.3, 40°C, recombinant wild-type enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20.7
with N-(3-(2-furyl)acryloyl)-Leu-Gly-Pro-Ala as substrate
22.42
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
substrate specificty is pH-dependent
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 8.5
activity range, profile overview
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 75
activity range, profile overview
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
67700
2 * 67700, enzyme without signal peptide, SDS-PAGE, 2 * 70200, enzyme with signal peptide, SDS-PAGE
69000
SDS-PAGE, purified enzyme
70200
2 * 67700, enzyme without signal peptide, SDS-PAGE, 2 * 70200, enzyme with signal peptide, SDS-PAGE
77050
sequence analysis
90000
-
gel filtration
98000
-
gel filtration
170000
gel filtration, native enzyme
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 70000, SDS-PAGE
homodimer
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
recombinant enzyme, stable at with over 60% of maximal activity
734466
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
stable for 1 h, thermally inactive at temperatures over 55°C
50
purified recombinant enzyme, 84% activity remaining
60
purified recombinant enzyme, 71% activity remaining after 1 h
70
purified recombinant enzyme, 30% activity remaining after 1 h
80
purified recombinant enzyme, no activity remaining after 1 h
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by chromatography
-
recombinant secreted enzyme from Escherichia coli strain BL21 (DE3) culture medium by dialysis, anion exchange and cation exchange chromatography
to homogeneity, 75fold
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from a Bacillus amyloliquefaciens 23-7A library
gene GT-SM3B, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the soluble enzyme with introduced N-terminal hexahistidine thrombin-cleavable tag in Escherichia coli strain BL21 (DE3) cytoplasm and secretion of the enzyme
gene pepF, DNA and amino acid sequence determination and analysis
gene pepF, DNA sequence determination and analysis, 2 putative promotor regions, operon expression is independent of the transcription regulator CtrA
-
overexpression in Bacillus subtilis
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information