Information on EC 3.4.24.77 - snapalysin

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The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY hide
3.4.24.77
-
RECOMMENDED NAME
GeneOntology No.
snapalysin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolyses proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
945859-47-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain AMT-3
-
-
Manually annotated by BRENDA team
strain AMT-3
-
-
Manually annotated by BRENDA team
strain K-OK-02
-
-
Manually annotated by BRENDA team
strain K-OK-02
-
-
Manually annotated by BRENDA team
strain TH-2
-
-
Manually annotated by BRENDA team
strain TH-2
-
-
Manually annotated by BRENDA team
strain 594
-
-
Manually annotated by BRENDA team
strain 594
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Arg-Gly-Phe-Phe + H2O
Arg-Gly + Phe-Phe
show the reaction diagram
-
-
-
-
?
Arg-Gly-Pro-Phe-Pro-Ile + H2O
Arg-Gly-Pro + Phe-Pro-Ile
show the reaction diagram
-
-
-
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro + H2O
Arg-Pro-Pro-Gly + Phe-Ser-Pro
show the reaction diagram
-
-
-
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg
show the reaction diagram
-
-
-
-
?
azocasein + H2O
?
show the reaction diagram
Azocoll + H2O
?
show the reaction diagram
Bovine serum albumin + H2O
?
show the reaction diagram
casein + H2O
?
show the reaction diagram
furylacryloyl-Met-Leu-Phe-Lys + H2O
furylacryloyl-Met-Leu + Phe-Lys
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
Glucagon + H2O
?
show the reaction diagram
Gly-Gly-Phe-Leu + H2O
Gly-Gly + Phe-Leu
show the reaction diagram
-
-
-
-
?
Insulin B-chain + H2O
?
show the reaction diagram
Leu-Trp-Met-Arg-Phe + H2O
Leu-Trp + Met-Arg-Phe
show the reaction diagram
-
-
-
-
?
Leu-Trp-Met-Arg-Phe-Ala + H2O
Leu-Trp + Met-Arg + Phe-Ala
show the reaction diagram
-
-
-
-
?
Met-Arg-Phe-Ala + H2O
Met-Arg + Phe-Ala
show the reaction diagram
-
-
-
-
?
Met-Gly-Trp-Met-Asp-Phe-NH2 + H2O
Met-Gly-Trp-Met-Asp + Phe-NH2
show the reaction diagram
-
-
-
-
?
milk protein + H2O
?
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Glu-Phe + H2O
N-benzyloxycarbonyl-Glu + Phe
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Gly-Phe + H2O
N-benzyloxycarbonyl-Gly + Phe
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
Phe-Phe-Phe-Phe + H2O
Phe-Phe + Phe-Phe
show the reaction diagram
-
-
-
-
?
resolufin-labeled casein + H2O
resolufin + casein
show the reaction diagram
-
-
-
-
?
Tyr-Gly-Gly-Phe-Leu + H2O
Tyr-Gly-Gly + Phe-Leu
show the reaction diagram
-
-
-
-
?
Tyr-Gly-Gly-Phe-Met + H2O
Tyr-Gly-Gly + Phe-Met
show the reaction diagram
-
-
-
-
?
Tyr-Pro-Phe-Pro + H2O
Tyr-Pro + Phe-Pro
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
10 mM, 1.37fold activity enhancement after 1 h at 55C
Co2+
-
0.1-1 mM, up to 3fold increase of activity
Mn2+
-
0.1-1 mM, up to 3fold increase of activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
antipain dihydrochloride
-
0.074 mM, 94.9% residual proteolytic activity
Ca2+
-
more than 2 mM
chymostatin
-
0.1 mM, 91.7% residual proteolytic activity
Cu2+
-
10 mM, 46% inhibition
diisopropyl fluorophosphate
-
-
FeSO4
-
2 mM, activity decreases to less than 20% of the maximum
iodoacetamide
-
-
N-bromosuccinimide
-
1 mM, 97% inhibition
N-[(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl]leucine
-
0.13 mM, 96.1% residual proteolytic activity
NiSO4
-
2 mM, activity decreases to less than 20% of the maximum
pefabloc
-
1 mM, 82.3% residual proteolytic activity
Phenanthroline
-
-
phenyl-methyl-sulfonyl-fluoride
-
-
phosphoramidon
-
0.6 mM, no residual proteolytic activity
thiorphan
-
0.001 mM and 0.0001 mM, 35% and 47% inhibition, respectively
Zn2+
-
0.01 mM
ZnSO4
-
2 mM, activity decreases to less than 20% of the maximum
additional information
-
no inhibitory effect of phenyl-methyl-sulfonyl-fluoride and p-chlormercuribenzoate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
0.005
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Ala-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Arg-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Glu-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Phe-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Asp-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Ile-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Lys-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Pro-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
5
N-[2-(N-methylamino)benzoyl]-2,3-diaminopropionic acid-Gly-Val-Tyr-Phe-Ala-Phe-Pro-Lys(2,4-dinitrophenyl)-D-Arg-D-Arg
Streptomyces septatus
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.25
-
supernatant
2.81
-
after 2.2fold purification
4.6
-
Suc-Ala-Ala-Phe-p-nitroanilide as substrate
5.3
-
bovine serum albumin as substrate
5.7
-
Gly-Gly-Gly-Phe-2-naphthylamide as substrate
6.4
-
beta-lactoglobulin as substrate
30.4
-
casein as substrate
33.3
-
gelatin as substrate
additional information
-
no degradation of Bz-L-Arg-2-naphthylamide, Bz-L-Tyr-2-naphthylamide, fyrylacryloyl-Gly-Leu-NH2, Z-Gly-Gly-Leu-p-nitroanilide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55 - 70
-
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15000 - 16000
-
gel filtration
16000
-
SDS-PAGE
20000 - 23000
-
SDS-PAGE
34060
-
calculated mass of the mature enzyme
40000
-
Superdex 200 10/300 GL gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure at 1 A resolution
-
crystal structure at 1.6 A resolution
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 80
-
calcium ion increases the activity by about 10% at the optimum temperature
30
-
during 30 min of various temperatures the enzyme is stable until 30C, but progressively lost its activity at higher temperature
30 - 40
-
the enzyme retains 90% of activity at 30C and 40C after 1 h incubation
60
-
stable at 60C for 120 min
additional information
-
Ca2+ plays an essential role in thermostability, but is not directly involved in the catalytic activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, crude enzyme preparation, 2 months, 25% loss of activity
-
4C, crude enzyme preparation, 2 months, 25% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
dialysis against 25 mM Tris-HCl, Vivapure-Q spin column chromatography and bio-scale CHT2-I hydroxyapatite column chromatography
-
gel filtration and ion exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation