Information on EC 3.4.24.74 - fragilysin

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The expected taxonomic range for this enzyme is: Bacteroides fragilis

EC NUMBER
COMMENTARY hide
3.4.24.74
-
RECOMMENDED NAME
GeneOntology No.
fragilysin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Broad proteolytic specificity, bonds hydrolysed including -Gly-/-Leu-, -Met-/-Leu-, -Phe-/-Leu-, -Cys-/-Leu-, Leu-/-Gly
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
-
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CAS REGISTRY NUMBER
COMMENTARY hide
188596-63-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Bacteroides fragilis 086-5443-2-2
strain 086-5443-2-2
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
strain I-1345
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Manually annotated by BRENDA team
strain NCTC 11295
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Abz-Asp-Tyr-Val-Ala-Ser-Glu-Lys(Dnp) + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-Gly-Ile-Val-Arg-Ala-Lys(Dnp) + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-Lys-Asp-Glu-Ser-Tyr-Arg-Lys(Dnp) + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-Thr-Val-Leu-Glu-Arg-Ser-Lys(Dnp) + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-Tyr-Gly-Lys-Arg-Val-Phe-Lys(Dnp) + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-Val-Lys-Phe-Tyr-Asp-Ile-Lys(Dnp) + H2O
?
show the reaction diagram
-
-
-
-
?
acetyl-Ala-Lys-Lys-Ala-Lys-Leu-Thr-Ala-Leu-Val-NMe + H2O
?
show the reaction diagram
-
-
-
-
?
actin + H2O
?
show the reaction diagram
Actin + H2O
Fragements of actin
show the reaction diagram
-
initial cleavage at Gly-Met, followed by Thr-Leu
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-
AGLRRAALGG + H2O
AGLRRAA + LGG
show the reaction diagram
-
-
-
-
?
alpha1-proteinase inhibitor + H2O
?
show the reaction diagram
-
-
-
-
?
azocasein + H2O
?
show the reaction diagram
Azocoll + H2O
Fragements of azocoll
show the reaction diagram
-
-
-
-
azocollagen + H2O
?
show the reaction diagram
-
-
-
-
?
beta-catenin + H2O
?
show the reaction diagram
-
-
-
-
?
BODIPY FL-casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
collagen type IV + H2O
?
show the reaction diagram
-
-
-
-
?
E-cadherin + H2O
?
show the reaction diagram
Fibrinogen + H2O
?
show the reaction diagram
Fibrinogen + H2O
Fragements of fibrinogen
show the reaction diagram
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hydrolysis to a lesser degree than other substrates
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-
Fibronectin + H2O
?
show the reaction diagram
Fragilysin + H2O
Fragements of fragilysisn
show the reaction diagram
-
autodigestion
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-
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
Fragements of gelatin
show the reaction diagram
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-
-
-
human complement C3 + H2O
?
show the reaction diagram
-
-
-
-
?
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(Dnp)-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
myosin + H2O
?
show the reaction diagram
-
-
-
-
?
myosinO + H2O
?
show the reaction diagram
-
-
-
?
p-phenylazobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg + H2O
?
show the reaction diagram
-
-
-
-
?
Proteins + H2O
?
show the reaction diagram
PTKIYDNIYD + H2O
PTKIY + DNIYD
show the reaction diagram
-
-
-
-
?
SGHMAALTA + H2O
SGHMAA + LTA
show the reaction diagram
-
-
-
-
?
SGPMSLRMTA + H2O
SGPMSLR + MTA
show the reaction diagram
-
-
-
-
?
SGPRSLKSTA + H2O
SGPRSLK + STA
show the reaction diagram
-
-
-
-
?
SGPVSMRYTA + H2O
SGPVSMR + YTA
show the reaction diagram
-
-
-
-
?
tropomyosin + H2O
?
show the reaction diagram
-
-
-
-
?
Tropomyosin + H2O
Fragements of tropomyosin
show the reaction diagram
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hydrolysis to a lesser degree than other substrates
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-
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
actin + H2O
?
show the reaction diagram
O05091, O86049, Q9S5W0
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-
-
?
E-cadherin + H2O
?
show the reaction diagram
Fibrinogen + H2O
?
show the reaction diagram
O05091, O86049, Q9S5W0
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-
-
?
myosinO
?
show the reaction diagram
O05091, O86049, Q9S5W0
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-
-
?
myosinO + H2O
?
show the reaction diagram
O05091, O86049, Q9S5W0
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-
-
?
Proteins + H2O
?
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
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-
5,7-dihydroxy-3',4',6-trimethoxyflavone
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inhibits the BFT induced nuclear factor-kappaB activation, interleukin-8 secretion, cyclo-oxygenase-2 expression and activated I-kappa-B-alpha and I-kappa-B-kinase signals in human colon epithelial cell line HT-29. Analysis of the mechanism of inhibition
Alpha-macroglobulin
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moderate inhibitor
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diethyl dicarbonate
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-
diethyldicarbonate
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moderate inhibitor
Diethylenetriaminepentaacetic acid
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-
dithiothreitol
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-
genistein
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broad spectrum tyrosine kinase inhibitor, significantly inhibits BFT-stimulated interleukin-8 production
germ agglutinin
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inhibits BFT biological activity on HT29/C1 cells but not binding of Alexa 488-labeled BFT to HT29/C1 cells
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GM6001
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significantly reduces Alexa 488-labeled BFT binding
PP2
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selective Src-family tyrosine kinase inhibitor, significantly inhibits ca. 73% of BFT-stimulated interleukin-8 production
SB203580
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p38 inhibitor, 0.01 mM inhibits ca. 80% of BFT-stimulated interleukin-8 production
subtilisin A
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inhibits both BFT biological activity on HT29/C1 cells and nearly 90% of binding of Alexa 488-labeled BFT
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Thermolysin
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inhibits both BFT biological activity on HT29/C1 cells and nearly 90% of binding of Alexa 488-labeled BFT
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thimerosal
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i.e. sodium ethylmercurithiosalicylate
tyrphostin AG1478
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selective epidermal growth factor receptor tyrosine kinase inhibitor, significantly inhibits ca. 64% of BFT-stimulated interleukin-8 production
U126
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inhibits both basal and BFT-augmented extracellular signal-related kinase phosphorylation, 0.01 mM inhibits ca. 80% of BFT-stimulated interleukin-8 production
additional information
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000 - 22000
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gel fitration
20600
-
cleaved to its enzymatically active secreted form
44400
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preproprotein
additional information
-
analysis of BFT-2 by native PAGE reveals bands at 36000 Da, 90000 Da and 200000 Da. Both the 36000 Da and higher-molecular-mass bands are biologically active when tested in the HT29/C1 cell assay
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
-
BFT-2 reveals a major band of 20000 Da with faint bands in some preparations at 70000 Da and 100000 Da, BFT-1 reveals a major band at 22000-23000 Da with fainter bands in some preparations at 70000 Da and 100000 Da, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 100 mM sodium citrate dihydrate, 20% (w/v) PEG 3000, pH 5.5
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
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-
668836
5 - 10
-
-
31497
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-20
-
stable at
4 - 20
-
unstable and rapidly autodigests above 37°C
4
-
stable at
55
-
unstable above
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
BFT-1 and BFT-2 are partially cleaved by trypsin, 1:2 w/w, after 24 h. The pattern of tryptic peptides from BFT-1 and BFT-2 is identical
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Stable to freeze-drying
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stable to treatment with trypsin, chymotrypsin, and Dispase I
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2000-3000fold purified by sequential ammonium sulfate precipitation, ion-exchange chromatography, hydrophobic chromatography, high resolution ion-exchange chromatography
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BFT-1 and VFT-2
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native enzyme from culture supernatant
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Ni Sepharose column chromatography and Superdex 200 gel filtration
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Ni-Sepharose column chromatography
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nickel-affinity column chromatography
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purified to homogeneity by urea-based purification method
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
analysis of the effect of enterotoxigenic Bacteroides fragilis in specific pathogen-free C57BL/6J mouse and germfree 129S6/SvEv mouse respectively
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determination of pathogenicity islet
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expressed in Escherichia coli B834(DE3) cells
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expressed in Escherichia coli BL21(DE3) Gold cells
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expressed in Escherichia coli Origami-2 (DE3) cells
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expression of H348D in Escherichia coli
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gene bft-1; gene bft-2; gene bft-3
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A394E
-
mutation does not affect BFT processing or secretion
G355R
-
expression of BFT similar to that of wild type
H348D
-
smaller amounts of BFT compared to wild type
H348Y/H352Y/H358Y
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no activity with E-cadherin
H358Y
-
expression of BFT similar to that of wild type
M366R
-
smaller amounts of BFT compared to wild type
E349A
-
residual activity against fibrinogen and fluorophore-labeled casein
-
H352Y
-
Alexa-488-labeled BFT binding to HT29/C1 not blocked
-
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
additional information