Information on EC 3.4.24.73 - jararhagin

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The expected taxonomic range for this enzyme is: Bothrops jararaca

EC NUMBER
COMMENTARY hide
3.4.24.73
-
RECOMMENDED NAME
GeneOntology No.
jararhagin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of -His10-/-Leu-, -Ala14-/-Leu-, -Tyr16-/-Leu-and -Phe24-/-Phe- bonds in insulin B chain
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
160477-79-2
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
additional information
-
jararhagin toxin binding to collagen appears to be sufficient to inhibit collagen-induced platelet aggregation in the human host
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Abz-LVEALYQ-EDDnp + H2O
?
show the reaction diagram
-
-
-
-
?
alpha2 subunit I domain of the platelet surface alpha2beta1 integrin + H2O
?
show the reaction diagram
-
-
-
-
?
alpha2-Plasmin inhibitor + H2O
Inactivated alpha2-plasmin inhibitor
show the reaction diagram
-
-
-
-
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
hydrolyzed casein
show the reaction diagram
-
-
-
-
collagen XII + H2O
?
show the reaction diagram
collagen XIV + H2O
?
show the reaction diagram
F-actin + H2O
G-actin + ?
show the reaction diagram
-
-
-
-
?
Fibrin + H2O
?
show the reaction diagram
-
-
-
-
?
Fibrinogen + H2O
?
show the reaction diagram
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
?
focal adhesion kinase + H2O
?
show the reaction diagram
-
-
-
-
?
FVNQHLCGSHLVEALYVCGERGFFYTPKA + H2O
FVNQHLCGSH + Lys-Val-Glu-Ala + Lys + YVCGERGF + FYTPKA
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
Laminin + H2O
?
show the reaction diagram
matrilin 1 + H2O
?
show the reaction diagram
matrilin 3 + H2O
?
show the reaction diagram
matrilin 4 + H2O
?
show the reaction diagram
Nidogen + H2O
?
show the reaction diagram
-
degradation, preferred substrate
-
-
?
Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
show the reaction diagram
-
cleavage at 4 peptide bonds
-
-
platelet collagen receptor integrin alpha2beta1 + H2O
?
show the reaction diagram
-
-
-
-
?
Type I collagen + H2O
?
show the reaction diagram
Type IV collagen + H2O
?
show the reaction diagram
von Willebrand factor + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
collagen XII + H2O
?
show the reaction diagram
-
-
-
-
?
collagen XIV + H2O
?
show the reaction diagram
-
-
-
-
?
Fibrinogen + H2O
?
show the reaction diagram
Laminin + H2O
?
show the reaction diagram
-
degradation
-
-
?
matrilin 1 + H2O
?
show the reaction diagram
-
-
-
-
?
matrilin 3 + H2O
?
show the reaction diagram
-
-
-
-
?
matrilin 4 + H2O
?
show the reaction diagram
-
-
-
-
?
Nidogen + H2O
?
show the reaction diagram
-
degradation, preferred substrate
-
-
?
Type I collagen + H2O
?
show the reaction diagram
P30431
-
-
-
?
Type IV collagen + H2O
?
show the reaction diagram
-
degradation
-
-
?
von Willebrand factor + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
a Zn2+-dependent proteinase
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
Alpha-macroglobulin
-
no effective inhibition
-
alpha2-Macroglobulin
-
-
-
BJ46a
-
SVMP inhibitor forming a monovalent complex with jararhagin thus inhibiting its hemorrhagic and catalytic activities
-
DM 43
-
SVMP inhibitor isolated from the venom of Didelphis marsupialis forming stable complexes with jararhagin thus inhibiting its hemorrhagic, fibrinolytic and proteolytic activities
-
metalloproteinase inhibitor POL 647
-
-
-
PO41
-
SVMP inhibitor isolated from the venom of Philander opossum forming stable complexes with jararhagin thus inhibiting its hemorrhagic, fibrinolytic and proteolytic activities
-
additional information
-
no inhibition by alpha2-macroblobulin
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0051 - 0.0297
Abz-LVEALYQ-EDDnp
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0105 - 0.14
Abz-LVEALYQ-EDDnp
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
400
-
purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
additional information
-
pI: 4.3
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28500
-
SDS-PAGE, catalytic domain of jararhagin
47000
-
calculation from amino acid sequence, deduced from mature jararhagin
additional information
-
deduced amino acid sequence compared with those of other toxins of the zinc metalloproteinases and disintegrin family
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 52000, SDS-PAGE
monomer
-
1 * 52000, Bothrops jararaca, SDS-PAGE
additional information
-
three-dimensional structure of jararhagin, structure-function relationship, molecular modeling and dynamics, comparison to berythractivase, overview
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
vapour-diffusion technique, space group P2(1)2(1)2(1), unit-cell parameters a : 73.7 A, b : 100.3 A, c : 133.4 A
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
inactivation below
31485
6 - 9
-
stable
31485
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
complete loss of proteolytic and hemorrhagic activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity column chromatography
-
native enzyme from venom
-
nickel affinity chromatography
-
phenyl Superose column chromatography and Mono-Q column chromatography
-
synthetic peptides designed based on the sequence of the cysteine-rich domain of jararhagin are purified to homogeneity by gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain BL21(DE3)
-
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
refolding after treatment with 6 M urea
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine