Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
The taxonomic range for the selected organisms is: Agkistrodon contortrix contortrix
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Fibrinogen + H2O
?
the enzyme has a direct proteolytic activity against the A alpha-chain of fibrinogen at positions Lys413 and Leu414
-
-
?
fibrinogen + H2O
fibrin + propeptide
4-amino-benzene-His-Thr-Glu-Lys-leu-Val-Thr-Ser-dinitrophenol + H2O
?
-
-
-
-
?
azocasein + H2O
?
-
-
-
-
?
bradykinin + H2O
Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg
-
-
-
-
?
Fibrin Aalpha-chain + H2O
?
-
human, cleavage at Lys413-Lys414, the Bbeta-chain is cleaved more slowly and the gamma-chain is minimally affected
-
-
?
Fibrinogen + H2O
?
-
fibrolase rapidly cleaves the A(alpha)-chain of fibrinogen and the B(beta)-chain at a slower rate, while it has no activity on the gamma-chain. The primary cleavage site at the alpha-chain ist he Lys-Leu bond at residues 413-414
-
-
?
fibrinogen + H2O
fibrin + propeptide
Fibrinogen Aalpha-chain + H2O
?
His-Thr-Glu-Ala-Leu-Val-Thr-Ser + H2O
?
-
-
-
-
?
His-Thr-Glu-Arg-Leu-Val-Thr-Ser + H2O
?
-
-
-
-
?
His-Thr-Glu-Asn-Leu-Val-Thr-Ser + H2O
?
-
-
-
-
?
His-Thr-Glu-Glu-Leu-Val-Thr-Ser + H2O
?
-
-
-
-
?
His-Thr-Glu-His-Leu-Val-Thr-Ser + H2O
?
-
-
-
-
?
His-Thr-Glu-Leu-Leu-Val-Thr-Ser + H2O
?
-
-
-
-
?
His-Thr-Glu-Lys-Leu-Val-Thr-Ser + H2O
?
-
cleaving sequence residues 410-417 from fibrinogen Aalpha-chain
-
-
?
His-Thr-Glu-Phe-Leu-Val-Thr-Ser + H2O
?
-
-
-
-
?
His-Thr-Glu-Pro-Leu-Val-Thr-Ser + H2O
?
-
-
-
-
?
His-Thr-Glu-Ser-Leu-Val-Thr-Ser + H2O
?
-
-
-
-
?
Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val + H2O
?
-
cleaving sequence residues 11-18 from insulin B-chain
-
-
?
low molecular weight kininogen + H2O
kallidin + ?
-
-
i.e. Lys-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
?
low-molecular weight kininogen + H2O
kallidin + ?
additional information
?
-
Fibrin + H2O
?
-
-
?
Fibrin + H2O
?
degradation of fibrin clots
-
-
?
Fibrin + H2O
?
fibrinolytic activity
-
-
?
Fibrin + H2O
?
-
fibrinolytic activity
-
-
?
fibrinogen + H2O
fibrin + propeptide
bovine substrate
-
-
?
fibrinogen + H2O
fibrin + propeptide
bovine substrate, fibrinogenolytic activity
-
-
?
Fibrin + H2O
?
-
fibrinolytic activity
-
-
?
Fibrin + H2O
?
-
fibrinolytic activity cleaving the Lys413-Leu414 bond
-
-
?
Fibrin + H2O
?
-
human substrate, fibrinolytic activity cleaving the Lys413-Leu414 bond
-
-
?
Fibrin + H2O
?
-
fibrinolase cleaves the alpha-chain more rapidly than the beta-chain of fibrin
-
-
?
fibrinogen + H2O
fibrin + propeptide
-
fibrinogenolytic activity
-
-
?
fibrinogen + H2O
fibrin + propeptide
-
human substrate, fibrinogenolytic activity
-
-
?
Fibrinogen Aalpha-chain + H2O
?
-
human
-
-
?
Fibrinogen Aalpha-chain + H2O
?
-
cleavage at Lys413-Leu414 (initial cleavage)
-
-
?
Fibrinogen Aalpha-chain + H2O
?
-
cleavage specificity is not dictated by the Lys-Leu bond per se, but by the surrounding sequence
-
-
?
Fibrinogen Aalpha-chain + H2O
?
-
the Bbeta-chain is cleaved more slowly and the gamma-chain is minimally affected
-
-
?
Insulin B-chain + H2O
?
-
cleavage between Ala14 and Leu15
-
-
?
Insulin B-chain + H2O
?
-
oxidized
-
-
?
Insulin B-chain + H2O
?
-
fibrinogenolytic activity cleaving the Leu11-Val-Glu-Ala-Leu-Tyr-Leu-Val18 peptide
-
-
?
low-molecular weight kininogen + H2O
kallidin + ?
-
-
-
-
?
low-molecular weight kininogen + H2O
kallidin + ?
-
initiates generation of bradykinin
-
-
?
additional information
?
-
fibrolase identified in multiple isoforms
-
?
additional information
?
-
-
fibrolase identified in multiple isoforms
-
?
additional information
?
-
the enzyme is a nonhemorrhagic zinc metalloprotease
-
-
?
additional information
?
-
the enzyme is a nonhemorrhagic zinc metalloprotease
-
-
?
additional information
?
-
-
the enzyme is a nonhemorrhagic zinc metalloprotease
-
-
?
additional information
?
-
chimeric Arg-Gly-Asp-fibrolase efficiently inhibits platelet aggregation in a dose-dependent manner and a prolonged incubation time obviously increased the inhibitory effect, in contrast to fibrolase
-
-
?
additional information
?
-
-
chimeric Arg-Gly-Asp-fibrolase efficiently inhibits platelet aggregation in a dose-dependent manner and a prolonged incubation time obviously increased the inhibitory effect, in contrast to fibrolase
-
-
?
additional information
?
-
-
no activity against a series of chromogenic 4-nitroanilide substrates
-
-
?
additional information
?
-
-
exhibits little if any hemorrhagic activity
-
-
?
additional information
?
-
-
does not activate protein C
-
-
?
additional information
?
-
-
does not activate plasminogen
-
-
?
additional information
?
-
-
does not activate plasminogen
-
-
?
additional information
?
-
-
direct fibrinolytic activity
-
-
?
additional information
?
-
-
thrombolysis in animals by snake venom alfimeprase, overview
-
-
?
additional information
?
-
-
alfimeprase is the recombinant truncated form of fibrolase from snake venom lacking the two N-terminal amino acids Glu and Gln
-
-
?
additional information
?
-
-
the enzyme is a nonhemorrhagic zinc metalloprotease, peptide substrate specificity, preference for Xaa-Leu and Xaa-Phe overview
-
-
?
additional information
?
-
-
fibrolase neither activates nor degrades plasminogen
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Guan, A.L.; Retzios, A.D.; Henderson, G.N.; Markland, F.S.
Purification and characterization of a fibrinolytic enzyme from venom of the southern copperhead snake (Agkistrodon contortrix contortrix)
Arch. Biochem. Biophys.
289
197-207
1991
Agkistrodon contortrix contortrix
brenda
Retzios, A.D.; Markland, F.S.
A direct-acting fibrinolytic enzyme from the venom of Agkistrodon contortrix contortrix: effects on various components of the human blood coagulation and fibrinolysis systems
Thromb. Res.
52
541-552
1988
Agkistrodon contortrix contortrix
brenda
Randolph, A.; Chamberlain, S.H.; Chu, H.L.C.; Retzios, A.D.; Markland, F.S.; Masiarz, F.R.
Amino acid sequence of fibrolase, a direct-acting fibrinolytic enzyme from Agkistrodon contortrix contortrix venom
Protein Sci.
1
590-600
1992
Agkistrodon contortrix contortrix
brenda
Loayza, S.L.; Trikha, M.; Markland, F.S.; Riquelme, P.; Kuo, J.
Resolution of isoforms of natural and recombinant fibrolase, the fibrinolytic enzyme from Agkistrodon contortrix contortrix snake venom, and comparison of their EDTA sensitivities
J. Chromatogr.
662
227-243
1994
Agkistrodon contortrix contortrix
brenda
Retzios, A.D.; Markland, F.S.
Fibrinolytic enzymes from the venoms of Agkistrodon contortrix contortrix and Crotalus basiliscus basiliscus: cleavage site specificity towards the alpha-chain of fibrin
Thromb. Res.
74
355-367
1994
Agkistrodon contortrix contortrix
brenda
Manning, M.C.
Sequence analysis of fibrolase, a fibrinolytic metalloproteinase from Agkistrodon contortrix contortrix
Toxicon
33
1189-1200
1995
Agkistrodon contortrix contortrix
brenda
Trikha, M.; Schmitmeier, S.; Markland, F.S.
Purification and characterization of fibrolase isoforms from venom of individual southern copperhead (Agkistrodon contortrix Contortrix) snakes
Toxicon
32
1521-1531
1994
Agkistrodon contortrix contortrix
brenda
Patton, L.M.; Pretzer, D.; Schulteis, B.S.; Saggart, B.S.; Tennant, K.D.; Ahmed, N.K.
Activity assays for characterizing the thrombolytic protein fibrolase
J. Biochem. Biophys. Methods
27
11-23
1993
Agkistrodon contortrix contortrix
brenda
Markland, F.S.; Morris, S.; Deschamps, J.R.; Ward, K.B.
Resolution of isoforms of natural and recombinant fibrinolytic snake venom enzyme using high performance capillary electrophoresis
J. Liq. Chromatogr.
16
2189-2201
1993
Agkistrodon contortrix contortrix
-
brenda
Pretzer, D.; Schulteis, B.; Vander Velde, D.G.; Smith, C.D.; Mitchell, J.W.; Manning, M.C.
Effect of zinc binding on the structure and stability of fibrolase, a fibrinolytic protein from snake venom
Pharm. Res.
9
870-877
1992
Agkistrodon contortrix contortrix
brenda
Pretzer, D.; Schulteis, B.S.; Smith, C.D.; Vander Velde, D.G.; Mitchell, J.W.; Manning, M.C.
Stability of the thrombolytic protein fibrolase: effect of temperature and pH on activity and conformation
Pharm. Res.
8
1103-1112
1991
Agkistrodon contortrix contortrix
brenda
Chen, H.M.; Guan, A.L.; Markland, F.S.
Immunological properties of the fibrinolytic enzyme (fibrolase) from southern copperhead (Agkistrodon contortrix contortrix) venom and its purification by immunoaffinity chromatography
Toxicon
29
683-694
1991
Agkistrodon contortrix contortrix
brenda
Guan, A.L.; Markland, F.S.
Isoelectric focusing in immobilized pH gradients of a snake venom fibrinolytic enzyme
J. Biochem. Biophys. Methods
16
215-226
1988
Agkistrodon contortrix contortrix
brenda
Bolger, M.B.; Swenson, S.; Markland, F.S., Jr.
Three-dimensional structure of fibrolase, the fibrinolytic enzyme from southern copperhead venom, modeled from the X-ray structure of adamalysin II and atrolysin C
AAPS Pharmsci.
3
E16
2001
Agkistrodon contortrix contortrix (P28891), Agkistrodon contortrix contortrix
brenda
Shi, J.; Zhang, S.T.; Zhang, X.J.; Xu, H.; Guo, A.G.
Expression, purification, and activity identification of alfimeprase in Pichia pastoris
Protein Expr. Purif.
54
240-246
2007
Agkistrodon contortrix contortrix (P28891)
brenda
Zhang, S.T.; Shi, J.; Zhao, J.; Qi, Y.F.; Guo, A.G.
Expression of soluble and functional snake venom fibrinolytic enzyme fibrolase via the co-expression of DsbC in Escherichia coli
Protein Pept. Lett.
13
559-563
2006
Agkistrodon contortrix contortrix (P28891), Agkistrodon contortrix contortrix
brenda
Swenson, S.; Markland, F.S.
Fibrolase
Toxin Rev.
25
351-378
2006
Agkistrodon contortrix contortrix
-
brenda
Toombs, C.F.; Deitcher, S.R.
Nonclinical and clinical characterization of a novel acting thrombolytic: alfimeprase
Toxin Rev.
25
379-392
2006
Agkistrodon contortrix contortrix
-
brenda
No authors listed
Alfimeprase
Drugs R. D.
9
185-190
2008
Agkistrodon contortrix contortrix
brenda
Fang, H.M.; Zhao, L.; Lu, P.; Chen, S.J.; Bao, Z.X.; Qin, Y.F.; Zhu, Z.Y.; Zhao, J.M.; Mai, J.; Zhang, S.T.
Modular design, expression and characterization of novel bifunctional mutants of fibrolase with combined platelet aggregation-inhibition and fibrinolytic activity
Protein J.
30
247-252
2011
Agkistrodon contortrix contortrix (P28891), Agkistrodon contortrix contortrix
brenda
Markland, F.S.; Swenson, S.
Fibrolase: trials and tribulations
Toxins
2
793-808
2010
Agkistrodon contortrix contortrix
brenda