Information on EC 3.4.24.62 - magnolysin

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.24.62
-
RECOMMENDED NAME
GeneOntology No.
magnolysin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of polypeptides with Arg or Lys in P1 and P2, e.g. to hydrolyse pro-oxytocin at -Lys-Arg-/-Ala-Val-. The specificity further depends on the organization of a beta-turn-alpha-helix of nine or more residues containing the paired basic amino acids near the centre
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
110353-43-0
-
162875-09-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Pro-oxytocin/neurophysin + H2O
?
show the reaction diagram
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
show the reaction diagram
[Ala10]-Pro-oxytocin/neurophysin(1-20) + H2O
?
show the reaction diagram
[Arg11,Lys12]-Pro-oxytocin/neurophysin(1-20) + H2O
?
show the reaction diagram
-
i.e. peptide VI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20)
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Pro-oxytocin/neurophysin + H2O
?
show the reaction diagram
-
involved in post-translational processing
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
metalloendopeptidase
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
weak, 1 mM, peptide I as substrate
Cd2+
-
weak, 0.1 mM, peptide I as substrate
Cys-Lys-Gly-Gly-Lys-Arg-Ala-Val-Lys-Cys
-
-
D-Arg12 derivate of pro-oxytocin/neurophysin (1-20)
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Divalent cation chelators
-
-
-
Leu-Gly-Gly-Lys-Arg-Ala-Val-Lys-Asp
-
-
p-(chloromercuri)-benzenesulfonic acid
p-Chloromercuriphenylsulfonic acid
Zn2+
-
1 mM, peptide I as substrate
[D-Arg12]-Pro-oxytocin/neurophysin(1-20)
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 0.2
peptide I
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
pituitary neurosecretory granule
Manually annotated by BRENDA team
additional information
-
secretory granule from neurohypophysis hypothalamus
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000 - 58000
-
bovine, gel filtration
52000
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bovine, PAGE, 2 isoforms, MW 52000 and 58000
55000 - 65000
58000
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bovine, PAGE, 2 isoforms, MW 52000 and 58000
60000
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human
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
affinity chromatography; from purified vesicles
-
from purified vesicles; partial
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partial
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