Information on EC 3.4.24.60 - dactylysin

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The expected taxonomic range for this enzyme is: Tetrapoda

EC NUMBER
COMMENTARY hide
3.4.24.60
-
RECOMMENDED NAME
GeneOntology No.
dactylysin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of peptides of at least six residues, with bulky hydrophobic residues in the P1' position. Shows a preference for hydrophobic doublets such as -Phe-/-Phe- and -Phe-/-Leu- in somatostatin-(1-14)-peptide and dynorphin A-(1-6)-peptide, respectively
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
139466-40-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(19-28)ANF peptide + H2O
?
show the reaction diagram
-
i.e. atrial natriuretic factor derived peptide, cleavage site: Gly20-Leu21
-
-
-
(20-28)ANF peptide + H2O
?
show the reaction diagram
-
i.e. atrial natriuretic factor derived peptide
-
-
-
(21-28)ANF octapeptide + H2O
?
show the reaction diagram
-
i.e. atrial natriuretic factor derived peptide
-
-
-
(22-28)ANF heptapeptide + H2O
?
show the reaction diagram
-
i.e. atrial natriuretic factor derived peptide
-
-
-
(23-28)ANF hexapeptide + H2O
?
show the reaction diagram
-
i.e. atrial natriuretic factor derived peptide
-
-
-
(5-11)Substance P heptapeptide + H2O
?
show the reaction diagram
-
i.e. Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, cleavage site: Gly9-Leu10
-
-
-
(5-28)ANF + H2O
ANF(5-25) + Phe26-Arg27-Tyr28
show the reaction diagram
(6-11)Substance P hexapeptide + H2O
?
show the reaction diagram
-
i.e. Gln-Phe-Phe-Gly-Leu-Met-NH2, cleavage site: Gly9-Leu10
-
-
-
(Arg0,Leu6)-enkephalin + H2O
?
show the reaction diagram
-
cleavage of Phe4-Leu5
-
-
?
(Leu5,Arg6)-enkephalin + H2O
YGGF + Leu-Arg
show the reaction diagram
-
cleavage of Phe4-Leu5
-
-
?
Angiotensin II + H2O
?
show the reaction diagram
ANP-(103-126) + H2O
ANP-(103-123) + Phe124-Arg125-Tyr126
show the reaction diagram
-
i.e. atrial natriuretic peptide, cleavage sites: Xaa-Phe, Xaa-Leu or Xaa-Ile bonds
-
-
Antibacterial peptide PGLa + H2O
?
show the reaction diagram
-
i.e. Gly-Met-Ala-Ser-Lys-Ala-Gly-Ala-Ile-Ala-Gly-Lys-Ile-Ala-Lys-Val-Ala-Leu-Lys-Ala-Leu-NH2, a peptide of Xenopus laevis skin secretion, cleavage site: Lys12-Ile13
-
-
-
Asp-Ala-Asp-Thr-Cys-[D-Arg8]-kermit + H2O
?
show the reaction diagram
-
model substrate, derivative of kermit, cleavage site: Ser12-Phe13
-
-
-
atrial natriuretic factor + H2O
LGCNS + Phe-Arg-Tyr
show the reaction diagram
-
cleavage of Ser-Phe
-
-
?
beta(1-40)Amyloid
?
show the reaction diagram
-
cleavage site: Gly33-Leu34
-
-
-
beta-amyloid derived peptide + H2O
?
show the reaction diagram
-
cleavage of Gly-Leu
-
-
?
Bradykinin + H2O
?
show the reaction diagram
dynorphin A1-16 + H2O
?
show the reaction diagram
-
cleavage of Phe-Leu
-
-
?
kermit + H2O
?
show the reaction diagram
-
cleavage of the peptide link Ser-Phe
-
-
?
Litorin + H2O
?
show the reaction diagram
Neurokinin A + H2O
?
show the reaction diagram
neurokinin A + H2O
HKTDSFVG + Leu-Met-NH2
show the reaction diagram
-
-
-
-
?
Neuromedin B + H2O
?
show the reaction diagram
-
i.e. Gly-Asn-Leu-Trp-Ala-Thr-Gly-His-Phe-Met-NH2, major cleavage site: His8-Phe9, minor site: Gly7-His8
-
-
-
Neuromedin C + H2O
?
show the reaction diagram
peptide PglA + H2O
?
show the reaction diagram
-
antimicrobial peptide of Xenopus laevis skin secretion predominantly cleaved at the Lys12-Ile13 bond
-
-
?
Somatostatin-14 + H2O
?
show the reaction diagram
Substance P + H2O
?
show the reaction diagram
substance P + H2O
RPKQQF + Phe-Gly + Leu-Met-NH2
show the reaction diagram
-
cleavage of Gly9-Leu10
-
-
?
[Arg0,Leu5,Arg6]enkephalin + H2O
?
show the reaction diagram
-
heptapeptide Arg-Tyr-Gly-Gly-Phe-Leu-Arg, cleavage site: Phe5-Leu6
-
-
-
[Arg0,Leu5]enkephalin + H2O
?
show the reaction diagram
-
hexapeptide Arg-Tyr-Gly-Gly-Phe-Leu, cleavage site: Phe4-Leu5
-
-
-
[D-Arg8]-kermit + H2O
?
show the reaction diagram
-
model substrate, derivative of kermit i.e. Asp-Val-Asp-Glu-Arg-Asp-Val-Arg-Gly-Phe-Ala-Ser-Phe-Leu-NH2, cleavage site: Ser12-Phe13
-
-
-
[Leu5,Arg6]enkephalin + H2O
?
show the reaction diagram
-
hexapeptide Tyr-Gly-Gly-Phe-Leu-Arg, cleavage site: Phe4-Leu5
-
-
-
[Leu5,Ile6]enkephalin + H2O
?
show the reaction diagram
-
hexapeptide Tyr-Gly-Gly-Phe-Leu-Ile, cleavage site: Phe4-Leu5
-
-
-
[Leu5,Lys6]enkephalin + H2O
?
show the reaction diagram
-
hexapeptide Tyr-Gly-Gly-Phe-Leu-Lys, cleavage site: Phe4-Leu5
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(24-28)ANF pentapeptide
-
i.e. Asn-Ser-Phe-Arg-Tyr, weak, substance P as substrate, inhibits cleavage of Gly9-Leu10 and Phe7-Phe8 bonds
(25-28)ANF tetrapeptide
-
i.e. Ser-Phe-Arg-Tyr, weak, substance P as substrate, inhibits cleavage of Gly9-Leu10 and Phe7-Phe8 bonds
(Leu5)-enkephalin
-
inhibitor of dactylysin towards substance P
(Met5)-enkephalin
-
inhibitor of dactylysin towards substance P
1,10-phenanthroline
Acetorphan
-
less efficient than phosphoramidon
benzamidine
beta(25-35)Amyloid peptide
-
i.e. Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met
beta(31-35)Amyloid pentapeptide
-
i.e. Ile-Ile-Gly-Leu-Met
beta(31-35)Amyloid-NH2
-
i.e. Ile-Ile-Gly-Leu-Met-NH2
CP-96345
-
non-peptide agonist of substance P, substance P as substrate, inhibits cleavage of Gly9-Leu10 bond
Kelatorphan
-
less efficient than phosphoramidon
N-ethylmaleimide
p-Chloromercuriphenylsulfonic acid
Pentapeptides reproducing or mimicking the COOH-terminal sequence of ANF
-
24-28 ANF as substrate
-
phosphoramidon
RP 67580
-
non-peptide agonist of substance P, substance P as substrate, inhibits cleavage of Gly9-Leu10 bond
thiorphan
tosyl-Arg methyl ester
[Ala26]-(21-28)ANF
-
weak
[Leu5]enkephalin
[Leu5]enkephalinamide
[Val26]-(21-28)ANF
-
weak
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.091
(19-28)ANF peptide
-
-
0.513
(20-28)ANF peptide
-
-
0.571
(21-28)ANF octapeptide
-
-
0.208
(22-28)ANF heptapeptide
-
-
0.37
(23-28)ANF hexapeptide
-
-
0.124
(5-11)Substance P heptapeptide
-
-
0.05
(5-28)ANF
-
-
0.011
(Arg0,Leu6)-enkephalin
-
-
0.079
(Leu5,Arg6)-enkephalin
-
-
0.063
angiotensin II
-
-
0.019
ANP
-
-
-
0.028
antibacterial peptide PGLa
-
-
-
0.05
Atrial natriuretic factor
-
-
-
0.125
beta-(1-40)amyloid
-
-
-
0.125
beta-amyloid derived peptide
-
-
0.041
bradykinin
-
-
0.05
dynorphin A
-
-
0.024
Litorin
-
-
0.42
neurokinin
-
-
-
0.125
Neurokinin A
-
-
0.021
Neuromedin B
-
-
0.022
Neuromedin C
-
-
0.028
PGLa
-
-
0.018
Somatostatin-14
-
-
0.02
Substance P
0.011
[Arg0,Leu5,Arg6]enkephalin
-
-
0.133
[Arg0,Leu5]enkephalin
-
-
0.12
[D-Arg8]-kermit
-
-
0.143
[Ile26](21-28)ANF octapeptide
-
(6-11)substance P hexapeptide
0.408
[Leu26](21-28)ANF octapeptide
-
-
0.079
[Leu5,Arg6]enkephalin
-
-
0.167
[Leu5,Ile6]enkephalin
-
-
0.2
[Trp26](21-28)ANF octapeptide
-
-
0.6
[Tyr26](21-28)ANF octapeptide
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0001
1,10-phenanthroline
-
98% inhibition
0.001
DTT
-
70% inhibition when tested on kermit
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.1826
-
[D-Arg8]-kermit as substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
about half-maximal activity at pH 6 and about 75% of maximal activity at pH 8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14000
-
subunit that retains the proteolytic activity
90000 - 100000
-
-
100000
-
1 * 100000, Xenopus laevis, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 100000, Xenopus laevis, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
24 h stable
31363
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Resistant to 1.5 M NaCl and 30% v/v methanol
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Methanol
-
resistant to 30% v/v methanol
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, in 10 mM potassium phosphate buffer, pH 7.5, 0.05% Nonidet P-40, at least 3 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2000fold purified by a four-step fractionation including successively ion-exchange column, hydrophobic chromatographies and gel filtration
-
improved procedure
-
partial
-