Information on EC 3.4.24.59 - mitochondrial intermediate peptidase

New: Word Map on EC 3.4.24.59
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
3.4.24.59
-
RECOMMENDED NAME
GeneOntology No.
mitochondrial intermediate peptidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
136447-30-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
cleavage of Notch recptor at the S5 site is abolished in MIEP knockout cells
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
+ H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
51 kDa subunit of complex I + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
ABC transporter of the mitochondrion 1 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
Abz-FQTKVAAK(Dnp)-NH2 + H2O
Abz-FQ + TKVAAK(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-FRSGQPLQNKVQLQ-ED(Dnp) + H2O
Abz-FRSGQPLQ + NKVQLQ-ED(Dnp)
show the reaction diagram
-
-
-
-
?
Abz-FSSKTTVK(Dnp)-NH2 + H2O
Abz-FS + SKTTVK(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-GFSPFRQ-EDDnp + H2O
Abz-GF + SPFRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-IKQSSLLK(Dnp)-NH2 + H2O
Abz-IKQ + SSLLK(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-MTAALKTK(Dnp)-NH2 + H2O
Abz-MT + AALK + TK(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-NLMKKSTK(Dnp)-NH2 + H2O
Abz-NLM + KKSTK(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-QPLANKVQ-ED(Dnp) + H2O
Abz-QPLA + NKVQ-ED(Dnp)
show the reaction diagram
-
-
-
-
?
Abz-QPLQAKVQ-ED(Dnp) + H2O
Abz-QPLQ + AKVQ-ED(Dnp)
show the reaction diagram
-
-
-
-
?
Abz-QPLQNKVQ-ED(Dnp) + H2O
Abz-QPLQ + NKVQ-ED(Dnp)
show the reaction diagram
-
-
-
-
?
Abz-RPPGFSPFRQ-EDDnp + H2O
Abz-RPPGF + SPFRQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-TTKLKAAK(Dnp)-NH2 + H2O
Abz-TTKL + L-Lys + L-Ala + AK(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-VAAQTKTK(Dnp)-NH2 + H2O
Abz-VAA + QTKTK(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-VISSRLEK(Dnp)-NH2 + H2O
Abz-VIS + SRLEK(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
Abz-VRNFRSGQPLQNKVQED(Dnp) + H2O
Abz-VRNFRSGQPLQ + NKVQED(Dnp)
show the reaction diagram
-
-
-
-
?
Abz-WTTGGKAK(Dnp)-NH2 + H2O
Abz-WT + TGGKAK(Dnp)-NH2
show the reaction diagram
-
-
-
-
?
alanine aminotransferase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
alanine aminotransferase 2 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
alanine:glyoxylate aminotransferase 3 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
aldehyde dehydrogenase 2B + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
aldehyde dehydrogenase 6B + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
alpha/beta-hydrolases superfamily protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
alternative oxidase 1D + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
arginase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
ascorbate peroxidase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
ATP synthase beta + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
ATP-dependent caseinolytic protease + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
B13 NADH complex protein + H2O
?
show the reaction diagram
substrate of isoform OCT1
-
-
?
Bradykinin + H2O
?
show the reaction diagram
-
-
-
-
?
coenzyme Q 3 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
cofactor of nitrate reductase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
copper ion binding protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
cytochrome c oxidase 4 precursor protein + H2O
mature cytochrome c oxidase + propeptide
show the reaction diagram
dihydrolipoamide acetyltransferase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
dihydrolipoamide succinyltransferase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
electron transfer flavoprotein alpha + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
FAD-linked oxidases family protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
frataxin + H2O
?
show the reaction diagram
-
-
-
-
?
FTSH protease 4 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
fumarase 1 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
glutamate tRNA synthetase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
glutathione reductase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
HSP60 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
Hsp60-3 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
Hsp70-1+ H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
Hsp70-2 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
iron-sulfur cluster biosynthesis family protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
isopentenyl diphosphate isomerase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
lactate/malate dehydrogenase family protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
lipoamide dehydrogenase 2 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
methylenetetrahydrofolate reductase family protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
mitochondrial acyl carrier protein 1 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
mitochondrial acyl carrier protein 2 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
mitochondrial acyl carrier protein 3 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
mitochondrial glycoprotein family protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
Mitochondrial intermediate proteins + H2O
?
show the reaction diagram
mitochondrial RNA helicase 2 + H2O
?
show the reaction diagram
substrate of isoform OCT1
-
-
?
monodehydroascorbate reductase 6 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
MPP beta + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
NAD(P)-binding Rossmann-fold superfamily protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
NADH-ubiquinone oxidoreductase-related protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
NADPH-dependent thioredoxin reductase B + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
Notch receptor + H2O
?
show the reaction diagram
-
Notch is proteolysed at the S5 site which is 8 amino acids downstream of the S3 site, generating a DELTA NICD (Notch intracellular domain) fragment
-
-
?
nuclear-encoded CLP protease P7 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
O-acetylserine (thiol) lyase isoform C + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
octapeptide from the mitochondrial processing peptidase-generated N-terminus in the substrate proteins + H2O
?
show the reaction diagram
-
-
-
-
?
Oxa1 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
pentatricopeptide repeat protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
peroxiredoxin IIF + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
phenylalanyl-tRNA synthetase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
polyketide + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
Precursor proteins that have already been processed by mitochondrial processing peptidase + H2O
?
show the reaction diagram
primosome PriB/single-strand DNA-binding protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
proteinaceous RNase P1 + H2O
?
show the reaction diagram
substrate of isoform OCT1
-
-
?
Proteins with the octapeptide Phe-Xaa-Xaa-Ser-Xaa-Xaa-Xaa-Xaa at the amino termini + H2O
Protein + Phe-Xaa-Xaa-Ser-Xaa-Xaa-Xaa-Xaa
show the reaction diagram
-
amino-terminal octapeptide can be cleaved only within the structural context of twice-cleaved precursors
-
-
-
radical SAM superfamily protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
ribosomal protein L3 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
Rieske iron sulfur protein from complex III + H2O
?
show the reaction diagram
substrate of isoform OCT1
-
-
?
rubredoxin-like superfamily protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
S-adenosyl-L-methionine-dependent methyltransferase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
succinate dehydrogenase 1-1 + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
succinyl-CoA ligase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
succinyl-CoA ligase alpha subunit + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
transketolase family protein + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
translation elongation factor Ts + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
UDP-3-O-acyl N-acetylglycosamine deacetylase + H2O
?
show the reaction diagram
substrate of isoform ICP55
-
-
?
valyl-tRNA synthetase + H2O
?
show the reaction diagram
substrate of isoform OCT1
-
-
?
Ygr031w/Imo32 + H2O
?
show the reaction diagram
-
Ygr031w/Imo32 encodes an Oct1 substrate protein with an unusual sequence motif
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cytochrome c oxidase 4 precursor protein + H2O
mature cytochrome c oxidase + propeptide
show the reaction diagram
-
enzyme inhibition leads to prevention of the normal processing of the precursor of cytochrome c oxidase, i.e. COX, subunit 4 and induced COX degradation, overview
-
-
?
frataxin + H2O
?
show the reaction diagram
-
-
-
-
?
Mitochondrial intermediate proteins + H2O
?
show the reaction diagram
Precursor proteins that have already been processed by mitochondrial processing peptidase + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
required
additional information
-
activated by divalent cations
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
total inhibition at 10 mM
amastatin
-
-
bestatin
-
significant inhibition
captopril
-
-
DTT
-
inhibits enzymatic activity above to 0.1 mM
iodoacetamide
-
-
iodoacetic acid
-
-
JA-2
-
-
Leupeptin
-
-
N-ethylmaleimide
-
-
p-hydroxymercuribenzoate
-
-
Pepstatin
-
-
Synthetic peptides
thiol-blocking agent
-
-
-
Thiol-blocking reagents
-
-
-
thiorphan
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DTT
-
activates enzymatic activity up to 0.1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0034
Abz-FQTKVAAK(Dnp)-NH2
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.000941
Abz-FRSGQPLQNKVQLQ-ED(Dnp)
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0013
Abz-FSSKTTVK(Dnp)-NH2
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.001
Abz-GFSPFRQ-EDDnp
-
pH 7.4, 37C, 0.1 mM DTT
0.0011
Abz-IKQSSLLK(Dnp)-NH2
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0042
Abz-MTAALKTK(Dnp)-NH2
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0013
Abz-NLMKKSTK(Dnp)-NH2
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0051
Abz-QPLANKVQ-ED(Dnp)
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0032
Abz-QPLQAKVQ-ED(Dnp)
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0029
Abz-QPLQNKVQ-ED(Dnp)
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.00085
Abz-TTKLKAAK(Dnp)-NH2
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.01
Abz-VAAQTKTK(Dnp)-NH2
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0019
Abz-VISSRLEK(Dnp)-NH2
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0018
Abz-VRNFRSGQPLQNKVQED(Dnp)
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0062
Abz-WTTGGKAK(Dnp)-NH2
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.057
Abz-FQTKVAAK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.122
Abz-FRSGQPLQNKVQLQ-ED(Dnp)
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.036
Abz-FSSKTTVK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.055
Abz-IKQSSLLK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.19
Abz-MTAALKTK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.028
Abz-NLMKKSTK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.057
Abz-QPLANKVQ-ED(Dnp)
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0943
Abz-QPLQAKVQ-ED(Dnp)
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.061
Abz-QPLQNKVQ-ED(Dnp)
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.07
Abz-TTKLKAAK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.05
Abz-VAAQTKTK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.083
Abz-VISSRLEK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.223
Abz-VRNFRSGQPLQNKVQED(Dnp)
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
0.0033
Abz-WTTGGKAK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17
Abz-FQTKVAAK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206909
130
Abz-FRSGQPLQNKVQLQ-ED(Dnp)
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206911
28
Abz-FSSKTTVK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206906
40
Abz-GFSPFRQ-EDDnp
Homo sapiens
-
pH 7.4, 37C, 0.1 mM DTT
8875
50
Abz-IKQSSLLK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206904
45
Abz-MTAALKTK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206903
22
Abz-NLMKKSTK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206905
11
Abz-QPLANKVQ-ED(Dnp)
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206913
29
Abz-QPLQAKVQ-ED(Dnp)
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206914
21
Abz-QPLQNKVQ-ED(Dnp)
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206912
82
Abz-TTKLKAAK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206908
5
Abz-VAAQTKTK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206901
44
Abz-VISSRLEK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206907
124
Abz-VRNFRSGQPLQNKVQED(Dnp)
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206910
0.53
Abz-WTTGGKAK(Dnp)-NH2
Homo sapiens
-
in 50 mM Tris, 100 mM NaCl, pH 8.0, at 25C
206902
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.057
captopril
-
pH 7.4, 37C
0.007
JA-2
-
pH 7.4, 37C
0.0015
thiorphan
-
pH 7.4, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6 - 8.9
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
-
assay at
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
initially MIP synthesized as a large precursor molecule carrying a mitochondrial leader peptide
Manually annotated by BRENDA team
-
encoding of MIP
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
74470
-
rat, calculation from amino acid sequence
78640
-
mass spectroscopy
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 75000, SDS-PAGE
monomer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70C, stable for at least 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography using Ni-Sepharose resin and ion exchange chromatography with a Hi-trap resource Q column
-
affinity purified
-
Ni-Sepharose column chromatography and Resource Q column chromatography
-
purified 2250fold to homogeneity
-
recombinant MIPEP protein is purified from baculovirus-infected Sf9 cells
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned into vector pG-3
-
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli BL21 (DE3) pLysS cells
-
expressed in Saccharomyces cerevisiae
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
MIP stimulates iron uptake by mitochondria
Show AA Sequence (126 entries)
Please use the Sequence Search for a certain query.