Information on EC 3.4.24.57 - O-sialoglycoprotein endopeptidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.4.24.57
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RECOMMENDED NAME
GeneOntology No.
O-sialoglycoprotein endopeptidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of O-sialoglycoproteins; cleaves -Arg31-/-Asp- bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
129430-53-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
CD43 knockout and CD43 wild type mice, on the C57BL/6 background
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CD43 + H2O
?
show the reaction diagram
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a O-linked glycoprotein on T-cell surface
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-
?
Cell-surface O-sialoglycoproteins + H2O
?
show the reaction diagram
Fetuin + H2O
N-terminal fragment of MW 45000 + a 20000 MW fragment at Val50 + C-terminal fragment at Val273 + C-terminal fragment at His287
show the reaction diagram
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Glycophorin A + H2O
?
show the reaction diagram
glycoprotein Ibalpha + H2O
?
show the reaction diagram
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extracellular GPIbalpha is removed by O-sialoglycoprotein endopeptidase treatment
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-
?
surface CD43 + H2O
?
show the reaction diagram
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OSGE improves T-cell function even in mice lacking CD43, showing that other glycoproteins must contribute to the OSGE effect on function, overview
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
surface CD43 + H2O
?
show the reaction diagram
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OSGE improves T-cell function even in mice lacking CD43, showing that other glycoproteins must contribute to the OSGE effect on function, overview
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-
?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zinc
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metalloprotease
additional information
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Staphylococcus aureus becomes more sensitive to the Zn2+ ion under the downregulation of glycoprotease expression in vitro
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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Ki is about 50 mM
High salt concentration
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irreversible, 0.3 M NaCl inactivates within a few h
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additional information
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not: phosphoramidon
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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Gcp may function independently from several key autolysins, e.g. Atl, LytM, and LytN, and regulators, e.g. ArlRS, Mgr/Rat, and CidA, overview
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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CD4+ T cell of both young and old mice
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
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Pasteurella haemolytica, calculation from amino acid sequence
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 8
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stable
31344
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Dilute solutions lose activity at 0-4°C, or on repeated freezing and thawing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C or -12°C, stable for over 1 year
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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construction of a spacp-regulated gcp expression strain and a TetR-regulated gcp antisense expression strain, the down-regulation of gcp expression can effectively inhibit Triton X-100-induced lysis, eliminate penicillin- and vancomycin-caused cell lysis, and dramatically increase tolerance to hydrolases, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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Gcp may be a target for developing antibacterial agents
medicine
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glycoprotease is a potential target for the development of antimicrobials against Staphylococcus aureus infection
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