Information on EC 3.4.24.57 - O-sialoglycoprotein endopeptidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.4.24.57
-
RECOMMENDED NAME
GeneOntology No.
O-sialoglycoprotein endopeptidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Hydrolysis of O-sialoglycoproteins; cleaves -Arg31-/-Asp- bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
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-
-
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Glycophorin A proteinase
-
-
-
-
Glycoprotease
-
-
-
-
Glycoprotease
-
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Glycoproteinase
-
-
-
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O-sialoglycoprotein endopeptidase
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OSGE
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Proteinase, glyco-
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-
-
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Sialoglycoprotease
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-
-
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Sialoglycoprotease
-
-
Sialoglycoproteinase
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-
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CAS REGISTRY NUMBER
COMMENTARY
129430-53-1
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
A1; serotype A13, A14 and A16, not: serotype A11 and all recognized T biotypes
-
-
Manually annotated by BRENDA team
Mannheimia haemolytica A1
A1
-
-
Manually annotated by BRENDA team
CD43 knockout and CD43 wild type mice, on the C57BL/6 background
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
sialidase and sialoglycoproteases modulate virulence in Porphyromonas gingivalis
physiological function
-
GPIbalpha-clustering activates caspase-9 activation and phosphatidylserine surface expression. This is opposed by O-sialoglycoprotein endopeptidase treatment
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CD43 + H2O
?
show the reaction diagram
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a O-linked glycoprotein on T-cell surface
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-
?
Cell-surface O-sialoglycoproteins + H2O
?
show the reaction diagram
-
no activity with glycoproteins which contain N-linked glycans but no O-sialo-glycans, leukocyte integrins, transferrin receptor, HLA class I and 8A3 Ag
-
-
-
Cell-surface O-sialoglycoproteins + H2O
?
show the reaction diagram
-
CD34, CD43 (leukosialin), CD44 (hyaluronic acid receptor) and CD45 (leukocyte common Ag)
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-
-
Cell-surface O-sialoglycoproteins + H2O
?
show the reaction diagram
Mannheimia haemolytica A1
-
no activity with glycoproteins which contain N-linked glycans but no O-sialo-glycans, leukocyte integrins, transferrin receptor, HLA class I and 8A3 Ag, CD34, CD43 (leukosialin), CD44 (hyaluronic acid receptor) and CD45 (leukocyte common Ag)
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-
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Fetuin + H2O
N-terminal fragment of MW 45000 + a 20000 MW fragment at Val50 + C-terminal fragment at Val273 + C-terminal fragment at His287
show the reaction diagram
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-
-
-
Glycophorin A + H2O
?
show the reaction diagram
-
-
-
-
-
Glycophorin A + H2O
?
show the reaction diagram
Mannheimia haemolytica A1
-
-
-
-
-
surface CD43 + H2O
?
show the reaction diagram
-
OSGE improves T-cell function even in mice lacking CD43, showing that other glycoproteins must contribute to the OSGE effect on function, overview
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-
?
glycoprotein Ibalpha + H2O
?
show the reaction diagram
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extracellular GPIbalpha is removed by O-sialoglycoprotein endopeptidase treatment
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-
?
additional information
?
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the enzyme removes the ligand for P-selectin from the surface of the myeloid cells, HL-60 cells and human neutrophils
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-
-
additional information
?
-
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not: furylacryloyl-Gly-Leu amide
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-
-
additional information
?
-
-
the enzyme is immunogenic and may have a role in inducing a protective immune response against the pathogen Pasteurella haemolytica
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-
-
additional information
?
-
-
the enzyme is required for staphylococcal growth in the culture
-
-
-
additional information
?
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Gcp is required for growth and is essential for bacteria, the essential Gcp is involved in the modification of substrates of murein hydrolases as well as in the regulation of expression and/or activity of some murein hydrolases, which, in turn, may play important roles in bacterial viability, Gcp may function independently from several key autolysins, e.g. Atl, LytM, and LytN, and regulators, e.g. ArlRS, Mgr/Rat, and CidA, overview
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-
-
additional information
?
-
-
increases alpha-CD3-induced Ca2+ responses in CD4+ T cells, and enhances cytokine production by CD4+ T cells, of both young and old mice, treatment with OSGE can enhance the expression of early activation markers in both WT and CD43 KO mice, overview
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-
-
additional information
?
-
-
removal of GPIbalpha N-terminal domain from human or mouse platelet GPIb-V-IX complex, overview
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-
-
additional information
?
-
Mannheimia haemolytica A1
-
the enzyme is immunogenic and may have a role in inducing a protective immune response against the pathogen Pasteurella haemolytica
-
-
-
additional information
?
-
Mannheimia haemolytica A1
-
not: furylacryloyl-Gly-Leu amide
-
-
-
additional information
?
-
Mannheimia haemolytica A1
-
the enzyme removes the ligand for P-selectin from the surface of the myeloid cells, HL-60 cells and human neutrophils
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
surface CD43 + H2O
?
show the reaction diagram
-
OSGE improves T-cell function even in mice lacking CD43, showing that other glycoproteins must contribute to the OSGE effect on function, overview
-
-
?
additional information
?
-
-
the enzyme is immunogenic and may have a role in inducing a protective immune response against the pathogen Pasteurella haemolytica
-
-
-
additional information
?
-
-
the enzyme is required for staphylococcal growth in the culture
-
-
-
additional information
?
-
-
Gcp is required for growth and is essential for bacteria, the essential Gcp is involved in the modification of substrates of murein hydrolases as well as in the regulation of expression and/or activity of some murein hydrolases, which, in turn, may play important roles in bacterial viability, Gcp may function independently from several key autolysins, e.g. Atl, LytM, and LytN, and regulators, e.g. ArlRS, Mgr/Rat, and CidA, overview
-
-
-
additional information
?
-
-
increases alpha-CD3-induced Ca2+ responses in CD4+ T cells, and enhances cytokine production by CD4+ T cells, of both young and old mice, treatment with OSGE can enhance the expression of early activation markers in both WT and CD43 KO mice, overview
-
-
-
additional information
?
-
Mannheimia haemolytica A1
-
the enzyme is immunogenic and may have a role in inducing a protective immune response against the pathogen Pasteurella haemolytica
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Zinc
-
metalloprotease
additional information
-
Staphylococcus aureus becomes more sensitive to the Zn2+ ion under the downregulation of glycoprotease expression in vitro
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-
Ki is about 50 mM
High salt concentration
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irreversible, 0.3 M NaCl inactivates within a few h
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additional information
-
not: phosphoramidon
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ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
Gcp may function independently from several key autolysins, e.g. Atl, LytM, and LytN, and regulators, e.g. ArlRS, Mgr/Rat, and CidA, overview
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Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
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-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Mannheimia haemolytica A1
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-
-
Manually annotated by BRENDA team
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CD4+ T cell of both young and old mice
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Mannheimia haemolytica A1
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-
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35000
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-
Pasteurella haemolytica, calculation from amino acid sequence
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
8
-
stable
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Dilute solutions lose activity at 0-4°C, or on repeated freezing and thawing
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STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4°C or -12°C, stable for over 1 year
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
construction of a spacp-regulated gcp expression strain and a TetR-regulated gcp antisense expression strain, the down-regulation of gcp expression can effectively inhibit Triton X-100-induced lysis, eliminate penicillin- and vancomycin-caused cell lysis, and dramatically increase tolerance to hydrolases, overview
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
drug development
-
Gcp may be a target for developing antibacterial agents
medicine
-
glycoprotease is a potential target for the development of antimicrobials against Staphylococcus aureus infection