Information on EC 3.4.24.54 - mucrolysin

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The expected taxonomic range for this enzyme is: Protobothrops mucrosquamatus

EC NUMBER
COMMENTARY hide
3.4.24.54
-
RECOMMENDED NAME
GeneOntology No.
mucrolysin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of Ser9-/-His, His10-/-Leu, Ala14-/-Leu, Leu15-/-Tyr and Tyr16-/-Leu bonds in insulin B chain
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
85898-38-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-Leu + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Leu + H2O
Alanine + leucine
show the reaction diagram
-
less effective than Gly-Pro, Leu-Gly or Gly-Leu
-
-
Dimethylcasein + H2O
?
show the reaction diagram
Fibrinogen + H2O
?
show the reaction diagram
-
-
-
-
?
Fibrinogen + H2O
Hydrolyzed fibrinogen
show the reaction diagram
-
hydrolyzes first the Aalpha and then the Bbeta chain
-
-
Gly-Gly + H2O
?
show the reaction diagram
-
-
-
-
?
Gly-Gly + H2O
Glycine + glycine
show the reaction diagram
-
less effective than Gly-Pro, Leu-Gly or Gly-Leu
-
-
Gly-Leu + H2O
Glycine + leucine
show the reaction diagram
-
-
-
-
Gly-Met + H2O
?
show the reaction diagram
-
-
-
-
?
Gly-Met + H2O
glycine + L-methionine
show the reaction diagram
-
less effective than Gly-Pro, Leu-Gly or Gly-Leu
-
-
-
Gly-Pro + H2O
?
show the reaction diagram
-
-
-
-
?
Gly-Pro + H2O
Glycine + proline
show the reaction diagram
-
-
-
-
Gly-Tyr + H2O
?
show the reaction diagram
-
-
-
-
?
Gly-Tyr + H2O
Glycine + tyrosine
show the reaction diagram
-
less effective than Gly-Pro, Leu-Gly or Gly-Leu
-
-
His-Leu + H2O
?
show the reaction diagram
-
-
-
-
?
His-Leu + H2O
Histidine + leucine
show the reaction diagram
-
less effective than Gly-Pro, Leu-Gly or Gly-Leu
-
-
insulin B chain + H2O
?
show the reaction diagram
-
hydrolyzes Aalpha chain first and then the Bbeta chain
-
-
?
Leu-Gly + H2O
?
show the reaction diagram
-
-
-
-
?
Leu-Gly + H2O
Leucine + glycine
show the reaction diagram
-
-
-
-
Leu-Tyr + H2O
?
show the reaction diagram
-
-
-
-
?
Leu-Tyr + H2O
Leucine + tyrosine
show the reaction diagram
-
less effective than Gly-Pro, Leu-Gly or Gly-Leu
-
-
Oxidized insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
-
cleavage at Ser9-His10, His10-Leu11, Ala14-Leu15, Leu15-Tyr16 and Tyr16-Leu17
-
-
Tyr-Leu + H2O
?
show the reaction diagram
-
-
-
-
?
Tyr-Leu + H2O
Tyrosine + leucine
show the reaction diagram
-
less effective than Gly-Pro, Leu-Gly or Gly-Leu
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
metalloproteinase, contains 3 mol/mol enzyme
Mg2+
-
metalloproteinase, contains 0.2 mol/mol enzyme
Zn2+
-
metalloproteinase, contains 2 mol/mol enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
Antivenin
-
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pI: 4.3
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
94000
-
x * 94000, Trimeresurus mucrosquamatus, SDS-PAGE
additional information
-
amino acid composition
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 94000, Trimeresurus mucrosquamatus, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
10 min, 55°C, inactivation
31300
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
10 min, pH 7, inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from lyophilized crude venom
-
to homogeneity, by gel filtraton and ion-exchange column chromatography
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
mucrolysin possesses lethal and hemorrhagic activities, induces coagulation necrosis, bleeding into various organs, especially the heart and stomach