Information on EC 3.4.24.53 - trimerelysin II

Word Map on EC 3.4.24.53
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Protobothrops flavoviridis

EC NUMBER
COMMENTARY hide
3.4.24.53
-
RECOMMENDED NAME
GeneOntology No.
trimerelysin II
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of Asn3-/-Gln, His10-/-Leu and Ala14-/-Leu in the insulin B chain, and the bond Z-Gly-Pro-/-Leu-Gly-Pro in a small molecule substrate of microbial collagenase
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
151125-15-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro + H2O
Benzyloxycarbonyl-Gly-Pro + Leu-Gly-Pro
show the reaction diagram
-
-
-
-
casein + H2O
hydrolyzed casein
show the reaction diagram
-
-
-
-
Fibrinogen + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-peptide-2,4-dinitroanilinoethylamide + H2O
?
show the reaction diagram
-
-
-
-
?
Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
show the reaction diagram
-
cleavage at Asn3-Gln4, His10-Leu11 and Ala14-Leu15
-
-
additional information
?
-
-
completely free from hemorrhagic acitivity
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Benzyloxycarbonyl-Gly-Leu
-
-
Benzyloxycarbonyl-Gly-Leu-NH2
-
-
Benzyloxycarbonyl-Gly-Pro-Leu
-
-
Benzyloxycarbonyl-Gly-Pro-Leu-Gly
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22
Benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 11
-
6: about 40% of activity maximum, 11: about 80% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 70
-
about 30% of activity maximum at 30C and 70C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22990
-
Trimeresurus flavoviridis, calculated from amino acid sequence
24000
-
Trimeresurus flavoviridis, low speed sedimentation
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
37C, 1 h, about 40% loss of activity
31297
7 - 9
-
37C, 1 h, stable
31297
11
-
37C, 1 h, about 60% loss of activity
31297
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
pH 8.5, 5 min, enzyme retains full activity up to 45C
60
-
pH 8.5, 5 min, complete loss of activity above
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
completely free from hemorrhagic activity