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Information on EC 3.4.24.51 - ophiolysin and Organism(s) Ophiophagus hannah and UniProt Accession A3R0T9

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.51 ophiolysin
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This record set is specific for:
Ophiophagus hannah
UNIPROT: A3R0T9 not found.
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The taxonomic range for the selected organisms is: Ophiophagus hannah
The expected taxonomic range for this enzyme is: Ophiophagus hannah
Reaction Schemes
Cleavage of Asn3-/-Gln, Gln4-/-His, His10-/-Leu, Ala14-/-Leu, and Tyr16-/-Leu in insulin B chain
Synonyms
ohagin, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ophiophagus metalloendopeptidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Fibrinogen + H2O
?
show the reaction diagram
casein + H2O
?
show the reaction diagram
-
-
-
-
?
insulin B chain + H2O
Phe-Val-Asn + Gln + HLCGSH + LVEA + Leu-Tyr + LVCGERGFFYTPKA
show the reaction diagram
-
-
-
-
?
Insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
-
cleavage of Asn3-Gln, Gln4-His, His10-Leu, Ala14-Leu and Tyr16-Leu
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Fibrinogen + H2O
?
show the reaction diagram
human substrate, specific degradation of the alpha-chain, no activity with beta-chain and gamma-chain, the enzyme has a fibrinogenolytic activity and hemorrhagic activity in mice, overview
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
complete inhibition
cysteine
-
strongly inhibits
formaldehyde
-
-
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
-
assay at
additional information
-
basic enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.84
sequence calculation
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
king cobra
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
VM3_OPHHA
611
0
69049
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47344
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation
50000
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation
55000
gel filtration
65000
70000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 50000, SDS-PAGE, 1 * 47344, sequence calculation
monomer
-
1 * 70000, Ophiophagus hannah, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
ohagin contains four potential N-glycosylation sites
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme by gel filtration, ion exchange and heparin affinity chromatography
by successive chromatographies
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic tree
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yamakawa, Y.; Omori-Satoh, T.
A protease in the venom of king cobra (Ophiophagus hannah): purification, characterization and substrate specificity on oxidized insulin B-chain
Toxicon
26
1145-1155
1988
Ophiophagus hannah
Manually annotated by BRENDA team
Yamakawa, Y.
Ophiolysin
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
699-701
2004
Ophiophagus hannah
-
Manually annotated by BRENDA team
Guo, X.; Zeng, L.; Lee, W.; Zhang, Y.; Jin, Y.
Isolation and cloning of a metalloproteinase from king cobra snake venom
Toxicon
49
954-965
2007
Ophiophagus hannah (A3R0T9), Ophiophagus hannah
Manually annotated by BRENDA team