Information on EC 3.4.24.50 - bothrolysin

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The expected taxonomic range for this enzyme is: Bothrops jararaca

EC NUMBER
COMMENTARY
3.4.24.50
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RECOMMENDED NAME
GeneOntology No.
bothrolysin
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cleavage of Gln4-/-His, Ser9-/-His and Ala14-/-Leu of insulin B chain and Pro-/-Phe of angiotensin I
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
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-
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Bothrops metalloendopeptidase J
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-
-
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J Protease
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-
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J Protease
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CAS REGISTRY NUMBER
COMMENTARY
443890-65-1
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
jararaca snake
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Angiotensin I + H2O
Hydrolyzed angiotensin I
show the reaction diagram
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cleavage of Pro-Phe
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angiotensin I + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
show the reaction diagram
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-
-
-
?
casein + H2O
?
show the reaction diagram
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-
-
-
?
insulin B chain + H2O
FVNQ + HLCGS + HLVEA + LYLVCGERGFFYTPKA
show the reaction diagram
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-
-
-
?
Insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
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cleavage of Gln4-His, Ser9-His and Ala14-Leu
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
activates
Ca2+
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increases reaction velocity, half-maximum activation at 0.4 mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
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+ 4 mM Ca2+: 85% inhibition
1,10-phenanthroline
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totally inhibits at 0.1 mM
5,5'-dithiobis(2-nitrobenzoic acid)
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1 mM: 70% inhibition, in presence of 2 mM Ca2+: 30% inhibition
DM43
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serum from opossum, inhibits the fibrinogenolytic activity of bothrolysin and forms a stable complex with it
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DTNB
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inhibitory, partially prevented by addition of Ca2+
EDTA
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0.1 mM: complete inhibition
Protein inhibitor
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from blood plasma of Bothrops jararaca, completely inhibits
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EDTA
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totally inhibits at 0.1 mM
additional information
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phosphoramidon, leupeptin, epoxysuccinyl derivatives e.g. E-64, Ep-475 or Ep-479, all 0.5 mM, are no inhibitors
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additional information
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not inhibited by phosphoramidon at 0.5 mM and E-64 and its derivates
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pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.8
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assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
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1 * 23000, Bothrops jararaca, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
by column chromatography
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