Information on EC 3.4.24.48 - ruberlysin

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The expected taxonomic range for this enzyme is: Crotalus ruber ruber

EC NUMBER
COMMENTARY hide
3.4.24.48
-
RECOMMENDED NAME
GeneOntology No.
ruberlysin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of His10-/-Leu, Ala14-/-Leu, Tyr16-/-Leu and Gly23-/-Phe bonds in the B chain of insulin; His-/-Pro, Pro-/-Phe, and Trp-/-Ser of angiotensin I; and Gly-/-Phe of Met enkephalin
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
846020-01-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Met)enkephalin + H2O
?
show the reaction diagram
-
cleavage at Gly3-Phe4
-
-
?
Angiotensin + H2O
?
show the reaction diagram
-
cleavage at Pro7-Phe8 and His6-Pro7
-
-
?
Angiotensin I + H2O
?
show the reaction diagram
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleaved at His-Pro and Pro-Phe
-
-
-
casein + H2O
?
show the reaction diagram
Dimethylcasein + H2O
?
show the reaction diagram
Fibrin + H2O
?
show the reaction diagram
-
-
-
-
-
Fibrinogen + H2O
?
show the reaction diagram
hide powder azure + H2O
?
show the reaction diagram
luteinizing hormone releasing hormone + H2O
?
show the reaction diagram
-
cleavage at Gly6-Leu7 and Trp3-Ser4
-
-
?
Luteinizing hormone-releasing hormone + H2O
?
show the reaction diagram
-
cleavage sites: Trp3-Ser4, Gly6-Leu7
-
-
-
Met-enkephalin + H2O
Tyr-Gly-Gly + Phe-Met
show the reaction diagram
-
i.e. Tyr-Gly-Gly-Phe-Met, cleavage site: Gly3-Phe4
-
-
oxidized insulin B chain + H2O
?
show the reaction diagram
-
-
-
-
?
Oxidized insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
-
cleavage sites: His10-Leu11, Ala14-Leu15, Tyr16-Leu17, Gly23-Phe24
-
-
additional information
?
-
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exhibits proteolytic, hemorrhatic and myonecrotic activity
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Na+
-
metalloproteinase, contains 10.3 mol Na+/mol enzyme
additional information
-
less than 0.3 mol Mg2+/mol enzyme and less than 0.9 mol K+/mol enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pI: 5.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by gel filtration and ion-exchange chromatography
-
from lyophilized crude venom
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
HT-2 has hemorrhagic and lethal activity