Information on EC 3.4.24.48 - ruberlysin

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The expected taxonomic range for this enzyme is: Crotalus ruber ruber

EC NUMBER
COMMENTARY
3.4.24.48
-
RECOMMENDED NAME
GeneOntology No.
ruberlysin
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cleavage of His10-/-Leu, Ala14-/-Leu, Tyr16-/-Leu and Gly23-/-Phe bonds in the B chain of insulin; His-/-Pro, Pro-/-Phe, and Trp-/-Ser of angiotensin I; and Gly-/-Phe of Met enkephalin
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Crotalus ruber metalloendopeptidase II
-
-
-
-
Hemorrhagic toxin II
-
-
-
-
Hemorrhagic toxin II
-
-
HT-2
-
-
-
-
red rattlesnake metalloendopeptidase
-
-
CAS REGISTRY NUMBER
COMMENTARY
846020-01-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
red rattlesnake
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Met)enkephalin + H2O
?
show the reaction diagram
-
cleavage at Gly3-Phe4
-
-
?
Angiotensin + H2O
?
show the reaction diagram
-
cleavage at Pro7-Phe8 and His6-Pro7
-
-
?
Angiotensin I + H2O
?
show the reaction diagram
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleaved at His-Pro and Pro-Phe
-
-
-
casein + H2O
?
show the reaction diagram
-
-
-
-
-
casein + H2O
?
show the reaction diagram
-
-
-
-
?
Dimethylcasein + H2O
?
show the reaction diagram
-
-
-
-
-
Dimethylcasein + H2O
?
show the reaction diagram
-
-
-
-
?
Fibrin + H2O
?
show the reaction diagram
-
-
-
-
-
Fibrinogen + H2O
?
show the reaction diagram
-
Aalpha chain, not beta or gamma chain
-
-
-
Fibrinogen + H2O
?
show the reaction diagram
-
hydrolysis of Aalpha and Bbeta chains, whereas the gamma chain is resistant to hydrolysis
-
-
?
hide powder azure + H2O
?
show the reaction diagram
-
-
-
-
-
hide powder azure + H2O
?
show the reaction diagram
-
-
-
-
?
luteinizing hormone releasing hormone + H2O
?
show the reaction diagram
-
cleavage at Gly6-Leu7 and Trp3-Ser4
-
-
?
Luteinizing hormone-releasing hormone + H2O
?
show the reaction diagram
-
cleavage sites: Trp3-Ser4, Gly6-Leu7
-
-
-
oxidized insulin B chain + H2O
?
show the reaction diagram
-
-
-
-
?
Oxidized insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
-
cleavage sites: His10-Leu11, Ala14-Leu15, Tyr16-Leu17, Gly23-Phe24
-
-
Met-enkephalin + H2O
Tyr-Gly-Gly + Phe-Met
show the reaction diagram
-
i.e. Tyr-Gly-Gly-Phe-Met, cleavage site: Gly3-Phe4
-
-
additional information
?
-
-
exhibits proteolytic, hemorrhatic and myonecrotic activity
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
metalloproteinase, contains 1.1 mol Ca2+/mol enzyme
Na+
-
metalloproteinase, contains 10.3 mol Na+/mol enzyme
Zn2+
-
metalloproteinase, contains 1.1 mol Zn2+/mol enzyme
Zn2+
-
1.1 mol of zinc per mol of protein
Ca2+
-
1.1 mol of calcium per mol of protein
additional information
-
less than 0.3 mol Mg2+/mol enzyme and less than 0.9 mol K+/mol enzyme
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
-
additional information
-
no inhibition by benzamidine, tosyl-L-Phe chloromethyl ketone, soybean or egg white trypsin inhibitor or PCMB
-
additional information
-
not inhibited by benzamidine
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
pI: 5.2
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
amino acid composition of isozyme HT-2; amino acid sequence (81% identity of toxin HT-2 and Crotalus atrox toxin Ht-d)
additional information
-
-
amino acid composition of isozyme HT-2
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 24000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE
?
-
x * 25000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
by gel filtration and ion-exchange chromatography
-
from lyophilized crude venom
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
HT-2 has hemorrhagic and lethal activity