Information on EC 3.4.24.47 - horrilysin

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Crotalus horridus horridus

EC NUMBER
COMMENTARY hide
3.4.24.47
-
RECOMMENDED NAME
GeneOntology No.
horrilysin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of only the single bond Ala14-/-Leu in the insulin B chain, Ser12-/-Leu in the A chain, and Ile-/-Gly, Pro-/-Ala, and Ser-/-Trp in melittin
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
84056-81-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Basement membrane preparation + H2O
?
show the reaction diagram
-
from glomeruli
-
-
-
Bee venom melittin + H2O
?
show the reaction diagram
-
cleaves at Ile2-Gly3, Pro14-Ala15, Ser18-Trp19
-
-
-
casein + H2O
?
show the reaction diagram
-
denatured casein, poor substrate
-
-
-
Collagen + H2O
?
show the reaction diagram
-
poor substrate
-
-
-
collagen I + H2O
?
show the reaction diagram
-
-
-
-
?
cow hide powder + H2O
?
show the reaction diagram
-
hydrolysis only at 20% of the rate at which hide powder azure is hydrolyzed
-
-
?
Denatured casein + H2O
?
show the reaction diagram
-
-
-
-
?
Fibrin + H2O
?
show the reaction diagram
Fibrinogen + H2O
?
show the reaction diagram
-
rapid digestion of the Aalpha chain followed by a slower degradation of the Bbeta chain
-
-
?
Fibrinogen + H2O
Hydrolyzed fibrinogen
show the reaction diagram
-
hydrolysis of alpha and beta chain
-
-
glomerular basement membrane + H2O
?
show the reaction diagram
-
catalysis of the complete solubilization
-
-
?
Hemoglobin + H2O
?
show the reaction diagram
Hide powder + H2O
?
show the reaction diagram
-
from cow, hydrolysis at 20% the rate of cow hide powder azure
-
-
-
hide powder azure + H2O
?
show the reaction diagram
insulin + H2O
?
show the reaction diagram
-
cleavage of the Val-Glu-Ala15-Leu16-Tyr-Leu bond of the B chain and Val-Cys-Ser12-Leu13-Tyr-Gly bond of the A chain
-
-
?
Melittin + H2O
?
show the reaction diagram
-
cleavage at the Ile2-Gly3, Pro14-Ala15 and Ser18-Trp19 bonds
-
-
?
Oxidized insulin A-chain + H2O
Hydrolyzed oxidized insulin A-chain
show the reaction diagram
-
cleavage site: Ser12-Leu13
-
-
Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
show the reaction diagram
-
cleavage site: Ala14-Leu15
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
hemorrhage inducing toxin
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
0.14 mol/mol enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
dithiothreitol
-
-
additional information
-
no inhibition by alpha1-antitrypsin, N-ethylmaleimide, iodoacetamide, phenylmethylsulfonylfluoride, egg white or soybean trypsin inhibitor
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
hide powder azure as substrate, 0.2 M Tris buffer
additional information
-
pI: 5.1
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
-
Crotalus horridus horridus, gel filtration
56000
-
1 * 56000, Crotalus horridus horridus, SDS-PAGE
additional information
-
amino acid composition compared to other rattlesnake venom proteins
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no glycoprotein
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Extensive dialysis against distilled water inactivates, incubation with Zn2+, Ca2+ or Mg2+ fails to reconstitute activity
-
Stable to dialysis against 0.2 M Tris buffer, pH 7.5
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, in either lyophilized or frozen state, at least 3 years
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by two step column chromatography
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
horrilysin possesses hemorrhagic and lethal activities