Information on EC 3.4.24.45 - atrolysin F

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The expected taxonomic range for this enzyme is: Crotalus atrox

EC NUMBER
COMMENTARY
3.4.24.45
-
RECOMMENDED NAME
GeneOntology No.
atrolysin F
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cleavage of Val2-/-Asn, Gln4-/-His, Leu6-/-Cys, His10-/-Leu, Ala14-/-Leu and Tyr16-/-Leu bonds in insulin B chain
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Crotalus atrox metalloendopeptidase f
-
-
-
-
Hemorrhagic toxin f
-
-
-
-
Hemorrhagic toxin f
-
-
CAS REGISTRY NUMBER
COMMENTARY
172306-52-4
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Western diamondback rattlesnake
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Basement membrane preparation + H2O
?
show the reaction diagram
-
-
-
-
-
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
-
involved in disruption of capillary membranes
-
-
-
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
-
produces not only local hemorrhage in skin and muscle, but also systemic hemorrhage in internal organs such as intestine, kidney, lung, heart and liver
-
-
-
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
-
together with atrolysin A member of the class P-III hemorrhagic toxins
-
-
-
casein + H2O
?
show the reaction diagram
-
-
-
-
-
Collagen IV + H2O
?
show the reaction diagram
-
basement membrane component
-
-
-
Dimethylcasein + H2O
?
show the reaction diagram
-
-
-
-
-
Dimethylcasein + H2O
?
show the reaction diagram
-
-
-
-
?
Fibrinogen + H2O
Hydrolyzed fibrinogen
show the reaction diagram
-
no coagulation
non-clottable products
-
Fibrinogen + H2O
Hydrolyzed fibrinogen
show the reaction diagram
-
cleaves gamma-chain, Aalpha and Bbeta-chains remain intact
-
-
-
Fibrinogen + H2O
Hydrolyzed fibrinogen
show the reaction diagram
-
cleaves gamma-chain, Aalpha and Bbeta-chains remain intact
non-clottable products
-
Fibrinogen + H2O
?
show the reaction diagram
-
-
-
-
?
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
-
Gelatin of collagen type I + H2O
?
show the reaction diagram
-
-
-
-
-
Gelatin of collagen type III + H2O
?
show the reaction diagram
-
-
-
-
-
Gelatin of collagen type IV + H2O
?
show the reaction diagram
-
-
-
-
-
Nidogen + H2O
?
show the reaction diagram
-
basement membrane component
-
-
-
Oxidized insulin B-chain + H2O
?
show the reaction diagram
-
-
-
-
?
Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
show the reaction diagram
-
-
-
-
-
Oxidized insulin B-chain + H2O
Hydrolyzed oxidized insulin B-chain
show the reaction diagram
-
cleaves Val2-Asn3, Gln4-His5, Leu6-Cys7, H10-Leu11, Ala14-Leu15, Tyr16-Leu17 bonds
several peptides
-
Laminin + H2O
?
show the reaction diagram
-
basement membrane component
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
no hydrolysis of tosyl-L-Arg methyl ester or N-benzoyl-L-Arg ethyl ester
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
-
involved in disruption of capillary membranes
-
-
-
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
-
produces not only local hemorrhage in skin and muscle, but also systemic hemorrhage in internal organs such as intestine, kidney, lung, heart and liver
-
-
-
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
-
together with atrolysin A member of the class P-III hemorrhagic toxins
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Zn2+
-
zinc metalloproteinase, 1 mol zinc/mol enzyme
Zn2+
-
essential for hemorrhagic and proteolytic activity; zinc metalloproteinase, 1 mol zinc/mol enzyme
Zn2+
-
1.15 mol per mol enzyme
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
-
1,10-phenanthroline
-
casein as substrate
1,10-phenanthroline
-
inhibits proteolytic activity
EDTA
-
casein as substrate
EDTA
-
inhibits proteolytic activity
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
pI: 7.7
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
64000
-
-
SDS-PAGE
additional information
-
-
amino acid composition, immunologically not related to hemorrhagic toxin a or c
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
1 * 64000, Crotalus atrox, SDS-PAGE
monomer
-
1 * 64000, Crotalus atrox, SDS-PAGE; in the presence of 2-mercaptoethanol
monomer
-
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
no glycoprotein
-
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
from lyophilized crude venom
-
ion-exchange chromatography and gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
second-most potent hemorrhagic toxin of all the atrolysins, systemic hemorrhage in lung, kidney, liver, heart, destruction of muscle tissue