Information on EC 3.4.24.42 - atrolysin C

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The expected taxonomic range for this enzyme is: Crotalus

EC NUMBER
COMMENTARY hide
3.4.24.42
-
RECOMMENDED NAME
GeneOntology No.
atrolysin C
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of His5-/-Leu, His10-/-Leu, Ala14-/-Leu, Tyr16-/-Leu and Gly23-/-Phe bonds in B chain of insulin. With small molecule substrates prefers hydrophobic residue at P2' and small residue such as Ala, Gly at P1
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
158886-17-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
red rattlesnake
-
-
Manually annotated by BRENDA team
Crotalus scutulatus subsp. scutulatus
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzyl amide + H2O
?
show the reaction diagram
Acetyl-Val-Ala-Ala-Leu-Gly-Ala + H2O
?
show the reaction diagram
-
-
-
-
-
Acetyl-Val-Ala-Leu-Leu-Ala + H2O
?
show the reaction diagram
-
best substrate of synthetic acetylated pentapeptides
-
-
-
Angiotensin I + H2O
Asp-Arg-Val-Tyr-Ile-His + Pro + Phe-His-Leu
show the reaction diagram
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleaved at His-Pro and Pro-Phe
-
-
azocoll
?
show the reaction diagram
-
-
-
-
?
Basement membrane preparation + H2O
Soluble peptides
show the reaction diagram
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
bovine aggrecan monomer + H2O
?
show the reaction diagram
cartilage aggrecan + H2O
?
show the reaction diagram
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
-
Collagen type IV + H2O
Hydrolyzed collagen type IV
show the reaction diagram
Dimethylcasein + H2O
?
show the reaction diagram
Fibrinogen + H2O
?
show the reaction diagram
-
-
-
-
?
Fibrinogen + H2O
Hydrolyzed fibrinogen
show the reaction diagram
-
-
-
-
-
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
-
FSFRLT + H2O
?
show the reaction diagram
-
good substrate
-
-
?
Gelatin of collagen type I + H2O
Hydrolyzed gelatin of collagen type I
show the reaction diagram
Gelatin of collagen type III + H2O
?
show the reaction diagram
-
i.e. denatured collagen
-
-
-
Gelatin of collagen type V + H2O
Hydrolyzed gelatin of collagen type V
show the reaction diagram
-
i.e. denatured collagen, hydrolysis at 38ųC and above, not below
MW 130000, MW 118000, MW 93000 and MW 85000 fragments
-
Glu-Ala-Leu-Tyr-Leu + H2O
?
show the reaction diagram
-
peptide derived from insulin B-chain
-
-
-
hide powder azure + H2O
?
show the reaction diagram
HTLRKA + H2O
?
show the reaction diagram
-
good substrate
-
-
?
Human alpha2-macroglobulin + H2O
?
show the reaction diagram
-
cleavage at Arg696-Leu697
-
-
-
IAQLNR + H2O
?
show the reaction diagram
-
good substrate
-
-
?
IPLRTV + H2O
?
show the reaction diagram
-
good substrate
-
-
?
Laminin + H2O
?
show the reaction diagram
Leu-Val-Glu-Ala-Leu-Tyr-Leu + H2O
Leu-Val-Glu-Ala + Leu-Tyr-Leu
show the reaction diagram
Luteinizing hormone-releasing hormone + H2O
?
show the reaction diagram
-
cleavage sites: Trp3-Ser4, Gly6-Leu7
-
-
-
Met-enkephalin + H2O
Tyr-Gly-Gly + Phe-Met
show the reaction diagram
-
i.e. Tyr-Gly-Gly-Phe-Met, cleavage site: Gly3-Phe4
-
-
-
Nidogen + H2O
?
show the reaction diagram
-
basement membrane component
-
-
-
Oxidized insulin B-chain + H2O
?
show the reaction diagram
SFMRLS + H2O
?
show the reaction diagram
-
good substrate
-
-
?
TQRKRS + H2O
?
show the reaction diagram
-
good substrate
-
-
?
Val-Glu-Ala-Leu-Tyr-Leu + H2O
?
show the reaction diagram
-
peptide derived from insulin B-chain
-
-
-
von Willebrand factor + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
von Willebrand factor + H2O
?
show the reaction diagram
-
VWF is a large, multidomain glycoprotein present in human blood and in the secretory granules of endothelial cells and platelets
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
0.75 mol/mol toxin HT-3, 1.1 mol/mol toxin HT-2
Na+
-
8.5 mol/mol toxin HT-3, 10.3 mol/mol toxin HT-2
additional information
-
less than 0.3 mol Mg2+/mol toxin, less than 0.8 mol K+/mol toxin HT-3 and less than 0.9 mol K+/mol toxin HT-2
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
Ala-Leu-Tyr-Leu
-
isozyme Ht-c
alpha2-Macroglobulin
Amino acid hydroxamates
amino acid hydroxymate
-
-
Benzyloxycarbonyl-Leu-Gly-NHOH
-
-
catrocollastatin C
-
-
-
chloromethyl ester
-
-
Collagenase inhibitor SC 44463
-
-
cysteine
-
-
Inhibitor SCH 47890
-
mechanism
phosphoramidon
Pyro-Glu-Asn-Trp
-
-
thiorphan
-
-
tissue inhibitor of metalloproteinase 3
-
N-terminal domain of the tissue inhibitor of metalloproteinase 3 is a potent inhibitor
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.71
acetyl-Ala-Ala-Leu-Gly-Ala
-
-
0.76
acetyl-Glu-Ala-Leu-Gly-Ala
-
isozyme Ht-c
0.69
Acetyl-Val-Ala-Ala-Leu-Gly-Ala
-
isozyme Ht-d
0.31 - 0.45
acetyl-Val-Ala-Leu-Gly-Gly
0.62 - 0.63
Acetyl-Val-Ala-Leu-Leu-Ala
0.5
acetyl-Val-Ala-Phe-Gly-Ala
-
isozyme Ht-c
0.21
acetyl-Val-Gly-Leu-Gly-Ala
-
isozyme Ht-c
0.14 - 0.29
Glu-Ala-Leu-Tyr-Leu
0.032 - 0.11
Leu-Val-Glu-Ala-Leu-Tyr-Leu
0.023 - 0.12
Val-Glu-Ala-Leu-Tyr-Leu
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00015
tissue inhibitor of metalloproteinase 3
-
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
8.3
-
hide powder azure, isozyme Ht-c
9
-
hide powder azure, isozyme Ht-d
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3 - 10
-
about half-maximal activity at pH 7.3 and 10, isozyme Ht-d
7.5 - 9.8
-
about half-maximal activity at pH 7.5 and 9.8, isozyme Ht-c
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20500
-
Crotalus atrox, isozyme Ht-c, gel filtration
21000
-
Crotalus atrox, isozyme Ht-d, gel filtration
23230
-
Crotalus atrox, isozyme Ht-d
24000
-
SDS-PAGE
27900
-
Crotalus atrox, minimal MW calculated from zinc content
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Crotalus atrox, crystallographic parameters
-
X-ray structure of atrolysin C, form D
-
-
Crotalus scutulatus subsp. scutulatus
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
formerly Ht-c and Ht-d; from lyophilized crude venom; two isozymes Cc and Cd
-
from lyophilized crude venom
-
from lyophilized crude venom, toxins HT-2 and HT-3
-
gel filtration, ion exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
molecular characteristics that influence the toxic effects of snake venom metalloproteases, subdivision in hemorrhagic and non-hemorrhagic snake venom metalloproteases