Information on EC 3.4.24.42 - atrolysin C

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The expected taxonomic range for this enzyme is: Crotalus

EC NUMBER
COMMENTARY hide
3.4.24.42
-
RECOMMENDED NAME
GeneOntology No.
atrolysin C
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of His5-/-Leu, His10-/-Leu, Ala14-/-Leu, Tyr16-/-Leu and Gly23-/-Phe bonds in B chain of insulin. With small molecule substrates prefers hydrophobic residue at P2' and small residue such as Ala, Gly at P1
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
158886-17-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
red rattlesnake
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzyl amide + H2O
?
show the reaction diagram
Acetyl-Val-Ala-Ala-Leu-Gly-Ala + H2O
?
show the reaction diagram
-
-
-
-
-
Acetyl-Val-Ala-Leu-Leu-Ala + H2O
?
show the reaction diagram
-
best substrate of synthetic acetylated pentapeptides
-
-
-
Angiotensin I + H2O
Asp-Arg-Val-Tyr-Ile-His + Pro + Phe-His-Leu
show the reaction diagram
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleaved at His-Pro and Pro-Phe
-
-
azocoll
?
show the reaction diagram
-
-
-
-
?
Basement membrane preparation + H2O
Soluble peptides
show the reaction diagram
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
bovine aggrecan monomer + H2O
?
show the reaction diagram
cartilage aggrecan + H2O
?
show the reaction diagram
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
-
Collagen type IV + H2O
Hydrolyzed collagen type IV
show the reaction diagram
Dimethylcasein + H2O
?
show the reaction diagram
Fibrinogen + H2O
?
show the reaction diagram
-
-
-
-
?
Fibrinogen + H2O
Hydrolyzed fibrinogen
show the reaction diagram
-
-
-
-
-
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
-
FSFRLT + H2O
?
show the reaction diagram
-
good substrate
-
-
?
Gelatin of collagen type I + H2O
Hydrolyzed gelatin of collagen type I
show the reaction diagram
Gelatin of collagen type III + H2O
?
show the reaction diagram
-
i.e. denatured collagen
-
-
-
Gelatin of collagen type V + H2O
Hydrolyzed gelatin of collagen type V
show the reaction diagram
-
i.e. denatured collagen, hydrolysis at 38ųC and above, not below
MW 130000, MW 118000, MW 93000 and MW 85000 fragments
-
Glu-Ala-Leu-Tyr-Leu + H2O
?
show the reaction diagram
-
peptide derived from insulin B-chain
-
-
-
hide powder azure + H2O
?
show the reaction diagram
HTLRKA + H2O
?
show the reaction diagram
-
good substrate
-
-
?
Human alpha2-macroglobulin + H2O
?
show the reaction diagram
-
cleavage at Arg696-Leu697
-
-
-
IAQLNR + H2O
?
show the reaction diagram
-
good substrate
-
-
?
IPLRTV + H2O
?
show the reaction diagram
-
good substrate
-
-
?
Laminin + H2O
?
show the reaction diagram
Leu-Val-Glu-Ala-Leu-Tyr-Leu + H2O
Leu-Val-Glu-Ala + Leu-Tyr-Leu
show the reaction diagram
Luteinizing hormone-releasing hormone + H2O
?
show the reaction diagram
-
cleavage sites: Trp3-Ser4, Gly6-Leu7
-
-
-
Met-enkephalin + H2O
Tyr-Gly-Gly + Phe-Met
show the reaction diagram
-
i.e. Tyr-Gly-Gly-Phe-Met, cleavage site: Gly3-Phe4
-
-
-
Nidogen + H2O
?
show the reaction diagram
-
basement membrane component
-
-
-
Oxidized insulin B-chain + H2O
?
show the reaction diagram
SFMRLS + H2O
?
show the reaction diagram
-
good substrate
-
-
?
TQRKRS + H2O
?
show the reaction diagram
-
good substrate
-
-
?
Val-Glu-Ala-Leu-Tyr-Leu + H2O
?
show the reaction diagram
-
peptide derived from insulin B-chain
-
-
-
von Willebrand factor + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
von Willebrand factor + H2O
?
show the reaction diagram
-
VWF is a large, multidomain glycoprotein present in human blood and in the secretory granules of endothelial cells and platelets
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
0.75 mol/mol toxin HT-3, 1.1 mol/mol toxin HT-2
Na+
-
8.5 mol/mol toxin HT-3, 10.3 mol/mol toxin HT-2
additional information
-
less than 0.3 mol Mg2+/mol toxin, less than 0.8 mol K+/mol toxin HT-3 and less than 0.9 mol K+/mol toxin HT-2
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
Ala-Leu-Tyr-Leu
-
isozyme Ht-c
alpha2-Macroglobulin
-
Amino acid hydroxamates
amino acid hydroxymate
-
-
Benzyloxycarbonyl-Leu-Gly-NHOH
-
-
catrocollastatin C
-
-
-
chloromethyl ester
-
-
Collagenase inhibitor SC 44463
-
-
cysteine
-
-
Inhibitor SCH 47890
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mechanism
phosphoramidon
Pyro-Glu-Asn-Trp
-
-
thiorphan
-
-
tissue inhibitor of metalloproteinase 3
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N-terminal domain of the tissue inhibitor of metalloproteinase 3 is a potent inhibitor
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.71
acetyl-Ala-Ala-Leu-Gly-Ala
-
-
0.76
acetyl-Glu-Ala-Leu-Gly-Ala
-
isozyme Ht-c
0.69
Acetyl-Val-Ala-Ala-Leu-Gly-Ala
-
isozyme Ht-d
0.31 - 0.45
acetyl-Val-Ala-Leu-Gly-Gly
0.62 - 0.63
Acetyl-Val-Ala-Leu-Leu-Ala
0.5
acetyl-Val-Ala-Phe-Gly-Ala
-
isozyme Ht-c
0.21
acetyl-Val-Gly-Leu-Gly-Ala
-
isozyme Ht-c
0.14 - 0.29
Glu-Ala-Leu-Tyr-Leu
0.032 - 0.11
Leu-Val-Glu-Ala-Leu-Tyr-Leu
0.023 - 0.12
Val-Glu-Ala-Leu-Tyr-Leu
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00015
tissue inhibitor of metalloproteinase 3
-
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
8.3
-
hide powder azure, isozyme Ht-c
9
-
hide powder azure, isozyme Ht-d
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3 - 10
-
about half-maximal activity at pH 7.3 and 10, isozyme Ht-d
7.5 - 9.8
-
about half-maximal activity at pH 7.5 and 9.8, isozyme Ht-c
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20500
-
Crotalus atrox, isozyme Ht-c, gel filtration
21000
-
Crotalus atrox, isozyme Ht-d, gel filtration
23230
-
Crotalus atrox, isozyme Ht-d
24000
-
SDS-PAGE
27900
-
Crotalus atrox, minimal MW calculated from zinc content
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Crotalus atrox, crystallographic parameters
-
X-ray structure of atrolysin C, form D
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
formerly Ht-c and Ht-d; from lyophilized crude venom; two isozymes Cc and Cd
-
from lyophilized crude venom
-
from lyophilized crude venom, toxins HT-2 and HT-3
-
gel filtration, ion exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
molecular characteristics that influence the toxic effects of snake venom metalloproteases, subdivision in hemorrhagic and non-hemorrhagic snake venom metalloproteases