Information on EC 3.4.24.41 - atrolysin B

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The expected taxonomic range for this enzyme is: Crotalus atrox

EC NUMBER
COMMENTARY hide
3.4.24.41
-
RECOMMENDED NAME
GeneOntology No.
atrolysin B
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of His5-/-Leu, His10-/-Leu, Ala14-/-Leu, Tyr16-/-Leu and Gly23-/-Phe of insulin B chain; identical to the cleavage of insulin B chain by atrolysin C. Also cleaves -/-Ser bonds in glucagon
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
172306-48-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Basement membrane preparation + H2O
Soluble peptides
show the reaction diagram
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
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involved in disruption of capillary membranes, together with atrolysins C and E member of the class P-I hemorrhagic toxins, most abundant hemorrhagic toxin in the venom
-
-
-
Dimethylcasein + H2O
?
show the reaction diagram
Fibrinogen + H2O
?
show the reaction diagram
-
-
-
-
?
Fibrinogen + H2O
Hydrolyzed fibrinogen
show the reaction diagram
-
-
-
-
-
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
Laminin + H2O
?
show the reaction diagram
Nidogen + H2O
?
show the reaction diagram
-
basement membrane component
-
-
-
o-aminobenzoyl-Ala-Gly-Leu-Ala-nitrobenzylamide + H2O
o-aminobenzoyl-Ala-Gly-Leu + Ala-nitrobenzylamide
show the reaction diagram
-
-
-
-
?
Oxidized insulin B-chain + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Basement membranes surrounding capillaries + H2O
?
show the reaction diagram
-
involved in disruption of capillary membranes, together with atrolysins C and E member of the class P-I hemorrhagic toxins, most abundant hemorrhagic toxin in the venom
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
Hemorrhagic toxin inhibitor from Crotalus atrox
-
-
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Oprin
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rosmarinic acid
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isolated from a methanolic extract of Argusia argentea (Boraginaceae) which significantly inhibits snake venom-induced hemorrhage; significantly inhibits the hemorrhagic effect of crude venom of Crotalus atrox and others as well as snake venom metalloproteinase HT-b
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0267 - 1.02
oxidized insulin B-chain
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19000
-
Crotalus atrox, gel filtration
29300
-
Crotalus atrox, minimal MW calculated from zinc content
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 24000, Crotalus atrox, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no glycoprotein
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by ion-exchange chromatography and gel filtration
-
from lyophilized crude venom
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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less potent hemorrhagic toxin, degradation of the basement membrane structure surrounding capillaries in muscle tissue