Information on EC 3.4.24.39 - deuterolysin

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.4.24.39
-
RECOMMENDED NAME
GeneOntology No.
deuterolysin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage of bonds with hydrophobic residues in P1'; also Asn3-/-Gln and Gly8-/-Ser bonds in insulin B chain
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
-
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CAS REGISTRY NUMBER
COMMENTARY hide
247028-11-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene Mep-1 like
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-
Manually annotated by BRENDA team
gene MEP1-like
Uniprot
Manually annotated by BRENDA team
gene NCU05071; gene NCU05071
UniProt
Manually annotated by BRENDA team
gene NCU05071; gene NCU05071
UniProt
Manually annotated by BRENDA team
gene MEP-2-like
UniProt
Manually annotated by BRENDA team
Uncinocarpus resii
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Albumin + H2O
?
show the reaction diagram
-
low activity of the enzyme
-
-
?
calf thymus histone 4 + H2O
?
show the reaction diagram
-
-
-
?
Carboxybenzyl-Gly-Phe amide + H2O
Carboxybenzyl-Gly + Phe amide
show the reaction diagram
-
-
-
-
casein + H2O
?
show the reaction diagram
-
low activity of the enzyme
-
-
?
casein + H2O
hydrolyzed casein
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
-
low activity of the enzyme
-
-
?
Hemoglobin + H2O
?
show the reaction diagram
-
low activity of the enzyme
-
-
?
histone + H2O
?
show the reaction diagram
-
high activity of the enzyme
-
-
?
Histone + H2O
Hydrolyzed histone
show the reaction diagram
-
-
-
-
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N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
-
-
-
?
N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
-
-
-
?
N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
-
-
-
?
Oxidized insulin B-chain + H2O
?
show the reaction diagram
protamine + H2O
?
show the reaction diagram
-
high activity of the enzyme
-
-
?
Protamine + H2O
Hydrolyzed protamine
show the reaction diagram
-
-
-
-
-
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
-
-
-
?
salmon protamine sulfate + H2O
?
show the reaction diagram
-
-
-
?
Z-Arg-Arg-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
metal chelator
-
-
-
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0264 - 0.0275
N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide
0.0638 - 0.07
N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide
0.146 - 0.1573
N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide
0.0119 - 0.0237
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide
0.0473 - 0.0522
Z-Arg-Arg-4-methylcoumarin-7-amide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.017 - 0.022
N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide
0.0017 - 0.0045
N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide
0.0035 - 0.009
N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide
0.00082 - 0.0022
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide
0.00065 - 0.0009
Z-Arg-Arg-4-methylcoumarin-7-amide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19300
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Aspergillus oryzae, gel filtration
19800
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recombinant enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 19000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 100
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extremely stable at 100C, but unstable near 75C because of self-digestion
70 - 100
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most unstable after 10 min at ca. 65-75C, but regains stability beyond this temperature
90
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10 min, less than 30% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant prodeuterolysin
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to homogeneity
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene BDCG_00922, sequence comparisons, and phylogenetic analysis and tree, overview; gene BDCG_03454, sequence comparisons, and phylogenetic analysis and tree, overview
C5G8P3, C5GGT9
gene CIMG_00508, sequence comparisons, and phylogenetic analysis and tree, overview; gene CIMG_03010, sequence comparisons, and phylogenetic analysis and tree, overview; gene CIMG_05736, sequence comparisons, and phylogenetic analysis and tree, overview; gene CIMG_07349, sequence comparisons, and phylogenetic analysis and tree, overview; gene CIMG_08613, sequence comparisons, and phylogenetic analysis and tree, overview; gene CIMG_10101, sequence comparisons, and phylogenetic analysis and tree, overview; gene CIMG_11800, sequence comparisons, and phylogenetic analysis and tree, overview
gene Mep-1 like, sequence comparisons, and phylogenetic analysis and tree, overview
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gene MEP-2-like, sequence comparisons, and phylogenetic analysis and tree, overview
gene MEP1-like, sequence comparisons, and phylogenetic analysis and tree, overview
gene MEP2, sequence comparisons, and phylogenetic analysis and tree, overview; gene MEP3, sequence comparisons, and phylogenetic analysis and tree, overview; gene MEP4-1 like, sequence comparisons, and phylogenetic analysis and tree, overview; gene MEP4, sequence comparisons, and phylogenetic analysis and tree, overview; gene MEP5, sequence comparisons, and phylogenetic analysis and tree, overview; gene MEP6, sequence comparisons, and phylogenetic analysis and tree, overview; gene MEP7, sequence comparisons, and phylogenetic analysis and tree, overview; gene MEP8, sequence comparisons, and phylogenetic analysis and tree, overview
gene NCU05071, sequence comparisons, and phylogenetic analysis and tree, overview
gene Q0UCJ2, sequence comparisons, and phylogenetic analysis and tree, overview; gene SNOG_02177, sequence comparisons, and phylogenetic analysis and tree, overview
gene UREG_01255, sequence comparisons, and phylogenetic analysis and tree, overview; gene UREG_04198, sequence comparisons, and phylogenetic analysis and tree, overview
Uncinocarpus resii
gene UREG_02006, sequence comparisons, and phylogenetic analysis and tree, overview; gene UREG_03761, sequence comparisons, and phylogenetic analysis and tree, overview
recombinant enzyme expressed in Escherichia coli
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recombinant NpII-O expressed from Saccharomyces cerevisiae as a secreted form and from Escherichia coli as a pro-NpII-O in the cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D104N
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site-directed mutagenesis
D121N
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site-directed mutagenesis
D143E
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site-directed mutagenesis
D143N
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site-directed mutagenesis
D164N
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site-directed mutagenesis
D80N
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site-directed mutagenesis
E129D
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site-directed mutagenesis
E129Q
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site-directed mutagenesis
E142Q
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site-directed mutagenesis
E42Q
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site-directed mutagenesis
E65Q
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site-directed mutagenesis
E86Q
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site-directed mutagenesis
H118A
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site-directed mutagenesis
H128R
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site-directed mutagenesis
H132R
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site-directed mutagenesis
R58Q
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site-directed mutagenesis
Y106F
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site-directed mutagenesis
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
likely enables organisms to utilize substrates in their environment
Show AA Sequence (519 entries)
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