Information on EC 3.4.24.38 - gametolysin

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The expected taxonomic range for this enzyme is: Chlamydomonas reinhardtii

EC NUMBER
COMMENTARY hide
3.4.24.38
-
RECOMMENDED NAME
GeneOntology No.
gametolysin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of the proline- and hydroxyproline-rich proteins of the Chlamydomonas cell wall; also cleaves azocasein, gelatin and Leu-Trp-Met-/-Arg-Phe-Ala
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
97089-74-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-neoendorphin + H2O
?
show the reaction diagram
-
-
-
-
?
azocasein + H2O
fragments of azocasein
show the reaction diagram
-
poor substrate
-
-
Azocoll + H2O
?
show the reaction diagram
-
-
-
-
-
casein + H2O
polypeptides derived from casein
show the reaction diagram
-
alpha- and beta-casein, poor substrate
-
-
Cell wall protein + H2O
?
show the reaction diagram
Chlamydomonas cell wall polypeptide + H2O
?
show the reaction diagram
-
2 polypeptides (peptide 2, MW 294000, and 5, MW 215000) of 16 polypeptides, i.e. the NaClO4-insoluble fraction, disintegrates cell wall structure by acting on only a few salt-insoluble components
-
-
-
dynorphin(1-13) + H2O
?
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
-
Heat-denatured collagen + H2O
?
show the reaction diagram
-
-
-
-
-
Leu-Trp-Met-Arg-Phe-Ala + H2O
Leu-Trp-Met + Arg-Phe-Ala + Leu-Trp + Met-Arg-Phe-Ala
show the reaction diagram
-
-
-
-
Mastoparan + H2O
?
show the reaction diagram
-
-
-
-
?
neurotensin + H2O
?
show the reaction diagram
-
-
-
-
?
Proteins rich in proline and hydroxyproline + H2O
?
show the reaction diagram
-
-
-
-
-
RNAse A + H2O
Lower MW polypetides
show the reaction diagram
-
-
-
-
Zoosporangia cell walls + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Cell wall protein + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zinc
-
zinc enzyme, 2.5 gatom zinc/62000 MW enzyme subunit, atomic absorption spectroscopy
additional information
-
the enzyme does not contain cobalt or manganese
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2,2'-dipyridyl
-
2 mM
Alpha-macroglobulin
-
-
-
alpha2-Macroglobulin
-
-
-
Co2+
-
above 1-2 mM, native or apoenzyme
diethyldicarbonate
-
-
Lectin from Canavalia ensiformis
-
not from Ricinus communis
-
Mn2+
-
above 1-2 mM, native or apoenzyme
p-chloromercuribenzoic acid
-
-
phosphoramidon
tosyl-Lys chloromethyl ketone
-
2 mM, less efficient than tosyl-Phe-chloromethyl ketone
tosyl-Phe chloromethyl ketone
-
i.e. TPCK, 2 mM
Zn2+
-
above 1-2 mM, native or apoenzyme
additional information
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 11
-
about half-maximal activity at pH 6 and 11, about 80% of maximal activity at pH 7 and 10.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
the enzyme, i.e. r-lysin, is released from periplasmic space during the first few min of mating
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49630
-
mature enzyme
60000 - 62000
-
active enzyme secreted into the mating medium
65000
-
proenzyme
67000
-
Chlamydomonas reinhardtii, gel filtration
additional information
-
each gamete mating type contains a single form of enzyme, the s-lysin of m+ is of slightly higher molecular mass than that of m-
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 67000, Chlamydomonas reinhardtii, SDS-PAGE
additional information
-
the enzyme is found as large aggregates with different molecular masses in culture medium or under low salt concentration, MW 120000, 180000, 220000 and higher
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
several h stable
31235
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
DTT stabilizes
-
Freeze-thawing of cells releases substantial amounts of inactive s-lysin in a soluble form
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, in Tris-acetate buffer, pH 7.5, 0.2 M NaCl, 2 mM DTT
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
freeze-thawing of cells releases substantial amounts of inactive s-lysin in a soluble form
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
-
degrades gametic cell walls, cell walls of vegetative cells and those of mother sporangial cells