Information on EC 3.4.24.37 - saccharolysin

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The expected taxonomic range for this enzyme is: Opisthokonta

EC NUMBER
COMMENTARY hide
3.4.24.37
-
RECOMMENDED NAME
GeneOntology No.
saccharolysin
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of Pro-/-Phe and Ala-/-Ala bonds
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
96779-48-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain CECT 12487, isolated from the natural microflora of fermented sausages and selected as possible starter culture on the basis of its physiological and biochemical properties and its ability to compete during sausage manufacturing processes
-
-
Manually annotated by BRENDA team
strain CECT 12487, isolated from the natural microflora of fermented sausages and selected as possible starter culture on the basis of its physiological and biochemical properties and its ability to compete during sausage manufacturing processes
-
-
Manually annotated by BRENDA team
Western corn rootworm
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ac-Ala-Ala-Pro-Met-NHPhNO2 + H2O
?
show the reaction diagram
-
-
-
-
?
Ac-Ala-Ala-Pro-Phe-NHPhNO2 + H2O
?
show the reaction diagram
-
-
-
-
?
Acetyl-Ala-Ala-Pro-Ala 4-nitroanilide + H2O
Acetyl-Ala + Ala-Pro-Ala 4-nitroanilide
show the reaction diagram
-
-
-
-
Acetyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
Acetyl-Ala + Ala-Pro-Phe 4-nitroanilide
show the reaction diagram
-
-
-
-
acetyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Benzoyl-Pro-Phe-Arg 4-nitroanilide + H2O
Benzoyl-Pro + Phe-Arg 4-nitroanilide
show the reaction diagram
-
-
-
-
Bradykinin + H2O
?
show the reaction diagram
-
cleaved at the Phe-Ser bond
-
-
?
Bz-Pro-Phe-Arg-NHPhNO2 + H2O
?
show the reaction diagram
-
-
-
-
?
Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys + H2O
?
show the reaction diagram
-
-
-
-
?
MeOSuc-Ala-Ala-Pro-Met-NHPhNO2 + H2O
?
show the reaction diagram
-
-
-
-
?
Methoxysuccinyl-Ala-Ala-Pro-Met 4-nitroanilide + H2O
Methoxysuccinyl-Ala + Ala-Pro-Met 4-nitroanilide
show the reaction diagram
-
-
-
-
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
Pz-Pro-Leu-Gly-Pro-D-Arg,Mcc-Pro-Leu-Gly-Pro-D-Lys(Dnp) + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
enzyme is not involved in any vital event in mitochondrial biogenesis
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
metalloprotease, not affeted by Cu2+, Co2+, Mn2+, and Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
complete inhibition at 0.1 mM
2-mercaptoethanol
-
19% inhibition at 5 mM
3,4-dichloroisocoumarin
-
71% inhibition at 1 mM
4-chloromercuribenzoate
-
complete inhibition at 0.1 mM
4-hydroxymercuribenzoate
-
100% inhibition with 0.1 mM
Cd2+
-
33% inhibition at 0.5 mM
Cpp-Ala-Ala-Phe-p-aminobenzoic acid
-
strongly inhibits
DTT
-
19% inhibition at 1 mM
EGTA
-
35% inhibition at 5 mM
Hg2+
-
complete inhibition at 0.05 mM
Mercurials
-
-
-
Mg2+
-
21% inhibition at 0.5 mM
Pepstatin
-
weak
pepstatin A
-
15% inhibition at 1 mM
PMSF
-
50% inhibition at 1 mM
Zn2+
-
73% inhibition at 0.5 mM
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetate
-
11% activation at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19
Ac-Ala-Ala-Pro-Met-NHPhNO2
-
-
0.085
Ac-Ala-Ala-Pro-Phe-NHPhNO2
-
-
0.19
Acetyl-Ala-Ala-Pro-Ala 4-nitroanilide
-
-
0.085
Acetyl-Ala-Ala-Pro-Phe 4-nitroanilide
-
-
0.06
benzoyl-Pro-Phe-Arg 4-nitroanilide
0.029
Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys
-
-
0.035
MeOSuc-Ala-Ala-Pro-Met-NHPhNO2
0.006
Pz-Pro-Leu-Gly-Pro-D-Arg
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6000
Acetyl-Ala-Ala-Pro-Ala 4-nitroanilide
Saccharomyces cerevisiae
-
-
38000
Acetyl-Ala-Ala-Pro-Phe 4-nitroanilide
Saccharomyces cerevisiae
-
-
1500
benzoyl-Pro-Phe-Arg 4-nitroanilide
Saccharomyces cerevisiae
-
-
38000
Methoxysuccinyl-Ala-Ala-Pro-Met 4-nitroanilide
Saccharomyces cerevisiae
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
methoxy-succinyl-Ala-Ala-Pro-Met 4-nitroanilide
5.8 - 6
-
acetyl-Ala-Ala-Pro-Ala 4-nitroanilide
6.5 - 7
-
benzoyl-Pro-Phe-Arg 4-nitroanilide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
with MeOSuc-Ala-Ala-Pro-Met-NHPhNO2
5.9
-
with Ac-Ala-Ala-Pro-Met-NHPhNO2
7.9
-
with Bz-Pro-Phe-Arg-NHPhNO2
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
midgut juice (pH 5.75) from third instars larvae
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68000
-
gel filtration
86000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
250fold by chromatographies
-
native enzyme 2560fold by fractionation with protamine sulfate, anion exchange chromatography on a weak followed by a strong anion exchange resin, and gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation