Information on EC 3.4.24.35 - gelatinase B

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
3.4.24.35
-
RECOMMENDED NAME
GeneOntology No.
gelatinase B
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of gelatin types I and V and collagen types IV and V
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
146480-36-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cattle infected with Echinococcus granulosus
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Gallus gallus White leghorn
-
UniProt
Manually annotated by BRENDA team
Mus musculus C57BL/6 x DBA/2
gene MMP9
UniProt
Manually annotated by BRENDA team
Mus musculus FVB/NJ
FVB/NJ mice
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-
Manually annotated by BRENDA team
-
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Manually annotated by BRENDA team
strain Y
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Manually annotated by BRENDA team
strain Y
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(7-methoxy-coumarin-4-yl)acetyl-PLGL-beta-(2,4-dinitrophenylamino)-AAR-NH2 + H2O
(7-methoxy-coumarin-4-yl)acetyl-PL + GL-beta-(2,4-dinitrophenylamino)-AAR-NH2
show the reaction diagram
-
-
-
-
?
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Leu-(3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-beta-(2,4-dinitrophenylamino)Ala-Ala-Arg-NH2 + H2O
?
show the reaction diagram
(7-methoxycoumaryl-4yl)acetylPLGLA2pr(2,4-dinitrophenol)-AR-NH2 + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-Pro-beta-cyclohexylalanyl-Gly-Cys(Me)-His-Ala-Lys(N-Me-2-aminobenzoyl)-NH2 + H2O
?
show the reaction diagram
-
a quenched fluorogenic substrate
-
-
?
2,4-Dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 + H2O
2,4-Dinitrophenyl-Pro-Leu-Gly + Leu-Trp-Ala-D-Arg-NH2
show the reaction diagram
-
and peptide substrates derived from, utility of substrate mapping form examining the subsite specificities and the optimization of proteinase substrates
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-
60 S ribosomal protein L6 + H2O
?
show the reaction diagram
-
-
-
-
?
Ac-L-Pro-L-Pro(OH)-Gly-L-Gln-Gly-L-Ala-L-Thr-Gly-L-Glu-L-Pro(OH)-Gly-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
actin + H2O
?
show the reaction diagram
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-
-
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?
ADAM 2 + H2O
?
show the reaction diagram
-
putative protein substrate with potential cleavage site
-
?
aggrecan + H2O
?
show the reaction diagram
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-
-
?
Aggrecan core protein + H2O
?
show the reaction diagram
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-
?
alpha 1(I) collagen + H2O
?
show the reaction diagram
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?
alpha 1(V) collagen + H2O
?
show the reaction diagram
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?
alpha 1(XI) collagen + H2O
?
show the reaction diagram
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-
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?
alpha 2(V) collagen + H2O
?
show the reaction diagram
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?
alpha-amylase precursor + H2O
?
show the reaction diagram
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?
alpha-enolase + H2O
?
show the reaction diagram
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?
alpha-N-acetylglucosaminidase + H2O
?
show the reaction diagram
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?
amyloid protein precursor + H2O
?
show the reaction diagram
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?
amyloid-beta A4 protein precursor + H2O
?
show the reaction diagram
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-
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?
annexin I + H2O
?
show the reaction diagram
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?
Arp2/3 complex subunit + H2O
?
show the reaction diagram
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?
arylsulfatase B precursor + H2O
?
show the reaction diagram
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-
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?
beta-glucuronidase precursor + H2O
?
show the reaction diagram
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?
beta-hexosaminidase beta chain precursor + H2O
?
show the reaction diagram
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?
beta1-integrin + H2O
?
show the reaction diagram
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?
beta2 integrin subunit CD18 + H2O
?
show the reaction diagram
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?
betaB1 crystallin + H2O
?
show the reaction diagram
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main enzyme substrate in lens extract
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?
Cartilage link protein + H2O
?
show the reaction diagram
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?
cathepsin E precursor + H2O
?
show the reaction diagram
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?
CB3 alpha 1(IV) collagen + H2O
?
show the reaction diagram
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?
CD25 + H2O
?
show the reaction diagram
-
CD25 is proteolytic cleaved to its soluble form by MMP-9
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?
CD316 antigen + H2O
?
show the reaction diagram
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?
chondroitin sulfate proteoglycan + H2O
?
show the reaction diagram
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?
citrate synthase + H2O
?
show the reaction diagram
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?
Collagen + H2O
?
show the reaction diagram
collagen I + H2O
?
show the reaction diagram
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?
Collagen type I + H2O
?
show the reaction diagram
collagen type II + H2O
?
show the reaction diagram
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?
Collagen type III + H2O
?
show the reaction diagram
collagen type IV + H2O
?
show the reaction diagram
collagen type V + H2O
?
show the reaction diagram
collagen type VII + H2O
?
show the reaction diagram
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?
collagen type XI + H2O
?
show the reaction diagram
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?
desmoglein 3 + H2O
?
show the reaction diagram
-
putative protein substrate with potential cleavage site
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?
DGRNIYNIHVEDSLECVKGPNVAA + H2O
?
show the reaction diagram
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?
ECVKGPNVAAIVGGT + H2O
ECVKGPNVAA + IVGGT
show the reaction diagram
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?
Elastin + H2O
?
show the reaction diagram
endoglin + H2O
?
show the reaction diagram
-
putative protein substrate with potential cleavage site
-
?
endothilin receptor + H2O
?
show the reaction diagram
-
putative protein substrate with potential cleavage site
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?
ephB1 + H2O
?
show the reaction diagram
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?
epidermal growth factor + H2O
?
show the reaction diagram
epididymis-specific alpha-mannosidase precursor + H2O
?
show the reaction diagram
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-
?
ezrin + H2O
?
show the reaction diagram
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?
fibrillar collagen + H2O
?
show the reaction diagram
Fibronectin + H2O
?
show the reaction diagram
filamin B + H2O
?
show the reaction diagram
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?
fragments of human collagen type II + H2O
?
show the reaction diagram
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fragments obtained by cleavage with collagenases MMP-1, MMP-8, or MMP-13. Enzyme produces small remnant peptides with still intact immunodominant epitopes
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?
galectin 3 + H2O
?
show the reaction diagram
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?
Galectin-3 + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
gelsolin + H2O
?
show the reaction diagram
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?
Golgi apparatus protein 1 precursor + H2O
?
show the reaction diagram
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?
heat shock protein 27 + H2O
?
show the reaction diagram
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?
heat shock protein 70 + H2O
?
show the reaction diagram
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?
heat shock protein 90 + H2O
?
show the reaction diagram
-
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-
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?
histidyl-tRNA synthetase + H2O
?
show the reaction diagram
-
-
-
-
?
HMGB1 protein + H2O
?
show the reaction diagram
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-
-
?
integrin beta5 + H2O
?
show the reaction diagram
-
putative protein substrate with potential cleavage site
-
?
interleukin-1beta + H2O
?
show the reaction diagram
-
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-
-
?
islet amyloid polypeptide + H2O
?
show the reaction diagram
kallikrein + H2O
?
show the reaction diagram
-
putative protein substrate with potential cleavage site
-
?
L-Pro-L-Pro(OH)-Gly-L-Gln-Gly-L-Ala-L-Thr-Gly-L-Glu-L-Pro(OH)-Gly-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
ladinin 1 + H2O
?
show the reaction diagram
-
putative protein substrate with potential cleavage site
-
?
Laminin + H2O
?
show the reaction diagram
laminin alpha3 chain + H2O
?
show the reaction diagram
-
putative protein substrate with potential cleavage site
-
?
lipase A + H2O
?
show the reaction diagram
-
-
-
-
?
LS276-THP + H2O
?
show the reaction diagram
-
development and evaluation of an activatable NIR fluorescent probe LS276-THP for in vivo detection of cancer-related matrix metalloproteinase activity based on a triplehelical peptide substrate with high specificity for MMP-2 and MMP-9 relative to other members of the MMP family, overview. Triple-helical peptides are suitable for highly specific in vivo detection of tumor-related MMP-2 and MMP-9 activity
-
-
?
moesin + H2O
?
show the reaction diagram
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?
myelin basic protein + H2O
?
show the reaction diagram
-
-
-
?
N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-7-methoxycoumarin-4-yl acetyl + H2O
?
show the reaction diagram
-
-
-
?
N-acetylglucosamine-6-sulfatase precursor + H2O
?
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-arginine p-nitroanilide + H2O
N-alpha-benzoyl-Arg + 4-nitroaniline
show the reaction diagram
-
i.e. BAPNA
-
-
?
N-cadherin + H2O
?
show the reaction diagram
-
-
-
-
?
Neonatal human proteoglycan + H2O
?
show the reaction diagram
-
poor substrate, cleavage of the His16-Ile17 bond
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-
-
nucleolin + H2O
?
show the reaction diagram
-
-
-
-
?
occludin + H2O
?
show the reaction diagram
-
-
-
-
?
pancreatic alpha-amylase isozyme + H2O
?
show the reaction diagram
-
-
-
-
?
peptide A13 + H2O
?
show the reaction diagram
-
-
-
?
peptide A13R + H2O
?
show the reaction diagram
-
-
-
?
peptide A3 + H2O
?
show the reaction diagram
-
-
-
?
peptide A34 + H2O
?
show the reaction diagram
-
-
-
?
peptide B74 + H2O
?
show the reaction diagram
-
-
-
?
peptide C15 + H2O
?
show the reaction diagram
-
non-selective peptide substrate
-
?
peptide C9 + H2O
?
show the reaction diagram
-
-
-
?
peptide m1A11 + H2O
?
show the reaction diagram
-
non-selective peptide substrate
-
?
peroxiredoxin 4 + H2O
?
show the reaction diagram
-
-
-
-
?
phosphate regulating neutral endopeptidase + H2O
?
show the reaction diagram
-
putative protein substrate with potential cleavage site
-
?
platelet factor 4 precursor + H2O
?
show the reaction diagram
-
-
-
-
?
pro-urokinase + H2O
active urokinase + prosequence of urokinase
show the reaction diagram
Proteoglycan + H2O
?
show the reaction diagram
-
-
-
-
?
retinoic acid early inducible protein 1 alpha-precursor + H2O
?
show the reaction diagram
-
-
-
-
?
retinoid-inducible serine carboxypeptidase precursor + H2O
?
show the reaction diagram
-
-
-
-
?
SGFGSRYLTA + H2O
?
show the reaction diagram
-
-
-
?
SGKGPRQITA + H2O
?
show the reaction diagram
-
-
-
?
SGKIPRRLTA + H2O
?
show the reaction diagram
-
-
-
?
SGKIPRTATA + H2O
?
show the reaction diagram
-
-
-
?
SGKIPRTLTA + H2O
?
show the reaction diagram
-
-
-
?
SGLKAMITA + H2O
?
show the reaction diagram
-
-
-
?
SGLPAKSTA + H2O
?
show the reaction diagram
-
-
-
?
SGPLFYSVTA + H2O
?
show the reaction diagram
-
-
-
?
SGPRAVSTTA + H2O
?
show the reaction diagram
-
-
-
?
SGQPHYLTTA + H2O
?
show the reaction diagram
-
-
-
?
snRNP D3 + H2O
?
show the reaction diagram
-
-
-
-
?
stathmin + H2O
?
show the reaction diagram
-
-
-
-
?
stromelysin 1 + H2O
?
show the reaction diagram
-
-
?
succinylated gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
tissue factor pathway inhibitor + H2O
?
show the reaction diagram
-
-
-
?
transforming growth factor-beta + H2O
?
show the reaction diagram
-
MMP9 mediates activation of latent transforming growth factor-beta
-
-
?
tubulin + H2O
?
show the reaction diagram
-
-
-
-
?
Type IV collagen + H2O
?
show the reaction diagram
-
-
-
-
?
type V collagen + H2O
?
show the reaction diagram
-
-
-
-
?
type XI collagen + H2O
?
show the reaction diagram
-
-
-
-
?
vascular endothelial-cadherin + H2O
?
show the reaction diagram
-
-
-
?
Vitronectin + H2O
?
show the reaction diagram
-
65000 MW and 75000 MW form of vitronectin
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Aggrecan core protein + H2O
?
show the reaction diagram
-
-
-
-
?
chondroitin sulfate proteoglycan + H2O
?
show the reaction diagram
-
-
-
-
?
Collagen + H2O
?
show the reaction diagram
collagen I + H2O
?
show the reaction diagram
-
-
-
-
?
Collagen type I + H2O
?
show the reaction diagram
-
-
-
-
?
Collagen type III + H2O
?
show the reaction diagram
-
-
-
-
?
collagen type IV + H2O
?
show the reaction diagram
collagen type V + H2O
?
show the reaction diagram
collagen type XI + H2O
?
show the reaction diagram
-
-
-
-
?
Elastin + H2O
?
show the reaction diagram
epidermal growth factor + H2O
?
show the reaction diagram
-
MMP-9 induces TGF-beta1 production in the airway epithelium through the cleavage of epidermal growth factor, EGF, and membrane-bound EGF-like ligands and activating epidermal growth factor receptor
-
-
?
fibrillar collagen + H2O
?
show the reaction diagram
Fibronectin + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
islet amyloid polypeptide + H2O
?
show the reaction diagram
Laminin + H2O
?
show the reaction diagram
pro-urokinase + H2O
active urokinase + prosequence of urokinase
show the reaction diagram
transforming growth factor-beta + H2O
?
show the reaction diagram
-
MMP9 mediates activation of latent transforming growth factor-beta
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Calcium
-
required
Zinc
-
required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2E)-3-(N-hydroxycarbamoyl)-2-(3-phenylpropylidene)propionyl-L-tryptophan-N-methylamide
-
-
(2E)-3-(N-hydroxycarbamoyl)-2-heptylidenepropionyl-L-tryptophan-N-methylamide
-
-
(2E)-3-(N-hydroxycarbamoyl)-2-isopropionyl-L-tryptophan-N-methylamide
-
-
(2E)-3-(N-hydroxycarbamoyl)-2-[(2E)-3-phenylprop-2-en-1-ylidene]propionyl-L-tryptophan-N-methylamide
-
-
(2E)-3-(N-hydroxycarbamoyl)-2-[(2E)-but-2-en-1-ylidene]propionyl-L-tryptophan-N-methylamide
-
-
(R)-2-(N-((6-fluoropyridin-3-yl)methyl)-4-methoxyphenyl-sulfonamido)-N-hydroxy-3-methylbutanamide
-
-
(S)-2,4-bis[(2,3-dihydroxybenzoyl)amino]butyric acid methyl ester
-
-
(S)-2,5-bis[(2,3-dihydroxybenzoyl)amino]pentanoic acid methyl ester
-
-
(S)-2,6-bis[(2,3-dihydroxybenzoyl)amino]-N-hydroxyhexanoamide
-
-
(S)-2,6-bis[(2,3-dihydroxybenzoyl)amino]hexanenitrile
-
-
(S)-2,6-bis[(2,3-dihydroxybenzoyl)amino]hexanoamide
-
shows 33% inhibition against MMP-9 at 0.00025 mM
(S)-2,6-bis[(2,3-dihydroxybenzoyl)amino]hexanoic acid
-
-
(S)-2,6-bis[(2,3-dihydroxybenzoyl)amino]hexanoic acid methyl ester
-
-
(S)-2,6-bis[(2,3-dimethoxybenzoyl)amino]hexanoic acid methyl ester
-
-
(S)-2,7-bis[(2,3-dihydroxybenzoyl)amino]heptanoic acid methyl ester
-
-
(S)-2-acetyl-6-[(2,3-dihydroxybenzoyl)amino]hexanoic acid methyl ester
-
-
(S)-2-benzoylamino-6-[(2,3-dihydroxybenzoyl)amino]-hexanoamide
-
-
(S)-2-benzoylamino-6-[(2,3-dihydroxybenzoyl)amino]-hexanoic acid methyl ester
-
-
(S)-6-benzoylamino-2-[(2,3-dihydroxybenzoyl)amino]-hexanoic acid methyl ester
-
-
1,10-phenanthroline
1,5-bis[(2,3-dihydroxybenzoyl)amino]pentane
-
-
2-[(biphenyl-4-ylsulfonyl)(isobutyl)amino]-N-hydroxyacetamide
-
50% inhibition at 34 nM, comparison with inhibitory effect on matrix metalloproteinases MMP-3, MMp-7, MMP-2
3',4',5,6,7,8-hexamethoxyflavone
-
i.e. nobiletin
3',4'-di-O-methylbutin-7-O-[(6''->1''')-3''',11'''-dimethyl-7'''-methylenedodeca-3''',10'''-dienyl]-beta-D-glucopyranoside
-
0.0065-0.0259 mM suppresses MMP-9 activity in lipopolysaccharide-stimulated human monocytic cells without cytotoxicity
3',4'-di-O-methylquercetin-7-O-[(4''->13''')-2''',6''',10''',14'''-tetramethylhexadec-13'''-ol-14'''-enyl]-beta-D-glucopyranoside
-
0.0065-0.0259 mM suppresses MMP-9 activity in lipopolysaccharide-stimulated human monocytic cells without cytotoxicity
3',4'-dihydroxy-5,6,7,8-tetramethoxyflavone
-
-
3'-hydroxy-4',5,6,7,8-pentamethoxyflavone
-
-
4'-hydroxy-3',5,6,7,8-pentamethoxyflavone
-
-
4'-O-methylbutin-7-O-[(6->1''')-3''',11'''-dimethyl-7'''-hydroxymethylenedodecanyl]-beta-D-glucopyranoside
-
0.0065-0.0259 mM suppresses MMP-9 activity in lipopolysaccharide-stimulated human monocytic cells without cytotoxicity
4'-O-methylkaempferol-3-O-[(4''->13''')-2''',6''',10''',14'''-tetramethylhexadecan-13'''-olyl]-beta-D-glucopyranoside
-
0.0065-0.0259 mM suppresses MMP-9 activity in lipopolysaccharide-stimulated human monocytic cells without cytotoxicity
5-(1,5-dihydroxypentan-3-yl)-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-hydroxybutan-2-yl)-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(3-(1-nitrooxy-methyl)piperidin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(4-(1-nitrooxy-ethyl)piperidin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(4-(2-nitrooxy-ethyl)piperazin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(4-(2-nitrooxy-ethyl)piperidin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(bis-(2-nitrooxy-ethyl)-amino)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(methyl-(2-nitrooxy-ethyl)-amino)-pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-[3-(hydroxymethyl)piperidin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-[4-(2-hydroxyethyl)piperazin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-[4-(2-hydroxyethyl)piperidin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-[4-(hydroxymethyl)piperidin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
adiponectin
-
adiponectin can markedly suppress protein expression and activity of MMP9 in brain, induced by 1 h ischemia followed by 23 h reperfusion
-
Ag-3340
-
i.e. N-hydroxy-2,2-dimethyl-4-[(4-phenoxyphenyl)sulfonyl]thiomorpholine-3-carboxamide, 50% inhibition at 0.26 nM, comparison with inhibitory effect on matrix metalloproteinases MMP-3, MMp-7, MMP-2
antibody REGA-3G12
-
highly selective, potent MMP-9 inhibition, the antibody recognizes the N-terminal part, not the C-terminal hemopexin and O-glycosylated domains, epitope mapping, overview
-
AZD-6140
-
-
BB1101
-
MMP inhibitor
BIIL284
-
BIIL284 treatment (3 mg/kg once daily for 2 weeks) significantly reduces the extracellular matrix metalloproteinase-9 activity in stented arteries
carboxy-derivatized glucosamine
-
62% inhibition of MMP-9 at 0.5 mg/ml
-
CGS 27023A
-
-
CGS27023A
-
50% inhibition at 8 nM, comparison with inhibitory effect on matrix metalloproteinases MMP-3, MMp-7, MMP-2
chitooligosaccharides
-
different molecular weight, inhibit both MMP-9 expression and activity, especially 1- to 3-kDa chitooligosaccharides, chitooligosaccharides-I in HT-1080 cells
cyclic CTT peptide
-
a gelatinase inhibitor peptide
cyclic CTTHWGFTLC
-
a specific gelatinase inhibitor
D-penicillamine
-
10 mg/l, 43% inhibition
doxycyclin
-
doxycycline significantly inhibits MMP-9 activity in gel zymography and also suppressed in situ gelatinase activity
doxycycline
GM6001
ilomastat
Immunoglobulins purified from antisera raised against gelatinase
-
-
-
macroglobulin
-
-
-
marimastat
-
-
Melatonin
-
i.e. N-acetyl-5-methoxytryptamine, used in protection during gastric ulcer, arresting cell injury, protein carbonyl formation, and lipid peroxidation in mice during gastroprotection, inhibits MMP-9 expression and activity
minocycline
-
0.02 mM minocycline displays inhibitory action on MMP-9 expression and activity
MMP-9 inhibitor I
MMP-9i
-
MMP-9 inhibitor, an anthranilate hydroxamic acid derivative
-
MMP9 inhibitor I
-
-
-
N,N'-[(2S)-6-[[(2,3-dihydroxyphenyl)carbonyl]amino]hexane-1,2-diyl]bis(2,3-dihydroxybenzamide)
-
-
N-hydroxy-2-(isobutyl[(4-methoxyphenyl)sulfonyl]amino)acetamide
-
50% inhibition at 2.9 nM, comparison with inhibitory effect on matrix metalloproteinases MMP-3, MMp-7, MMP-2
N-[(5R)-5-[[(2,3-dihydroxyphenyl)carbonyl]amino]-6-hydroxyhexyl]-2,3-dihydroxybenzamide
-
-
N-[(5S)-5-[[(2,3-dihydroxyphenyl)carbonyl]amino]-6-hydroxyhexyl]-2,3-dihydroxybenzamide
-
-
N-[(5S)-6-amino-5-[[(2,3-dihydroxyphenyl)carbonyl]amino]hexyl]-2,3-dihydroxybenzamide
-
-
norcantharidin
-
i.e. exo-7-oxabicylo-[2.2.1] heptane-2,3-dicarboxylic anhydride, norcantharidin inhibits gelatinase activity of MM-9 in a concentration- and time-dependent manner
o-phenanthroline
-
-
Phenanthroline
-
-
pioglitazone
-
pioglitazone reduces the upregulation of active form of MMP-9 after ischemia
polyhistidine
-
0.001 mM, 68% inhibition
proanthocyanidins
-
from the american cranberry, Vaccinium macrocarpon, decrease cellular viability of DU-145 cells via inhibition of MMP-9. The proanthocyanidins PACs increase the expression of TIMP-2, a known inhibitor of MMP activity, and decrease the expression of EMMPRIN, an inducer of MMP expression. Cranberry proanthocyanidins decrease MMP activity through the induction and/or inhibition of specific temporal MMP regulators, and by affecting either the phosphorylation status and/or expression of MAP kinase, PI-3 kinase, NF-kappaB and AP-1 pathway proteins
-
procyanidin oligomers
-
from Japanese quince, Chaenomeles japonica, fruit inhibit activity of MMP-9
-
REGA-3G12 scFv with (His6)2
-
monoclonal antibody REGA-3G12 single-chain variable fragment, 0.005 mM, 66% inhibition
-
reversion-inducing cysteine-rich protein with Kazal motifs
-
-
-
SB-3CT
TIMP-1
TIMP-1/2
-
-
-
TIMP-2
TIMP-4
-
-
-
TIMP1
-
interaction analysis and structure modeling, overview
-
TIMP2
-
interaction analysis and structure modeling, overview
-
Tissue inhibitor
tissue inhibitor of matrix metalloproteinase 1
-
strong inhibitor of MMP-9
-
tissue inhibitor of matrix metalloproteinase-1
-
TIMP-1
-
tissue inhibitor of metalloproteinase 1
-
-
-
tissue inhibitor of metalloproteinase TIMP-1
-
-
tissue inhibitor of metalloproteinase TIMP-2
-
-
Tissue inhibitor of metalloproteinase-1
Tissue inhibitor of metalloproteinase-2
-
-
-
tissue inhibitor of metalloproteinases 1
tissue inhibitor of metalloproteinases 2
-
TIMP-2, complete inhibition at 0.5 mg
-
tissue inhibitor of metalloproteinases-1
-
i.e. TIMP-1, plays a crucial role in the pathogenesis of hepatic fibrosis and thus may represent an important therapeutic target in the design of anti-fibrotic strategiesfor chronic liver disease, overview, molecular modeling of the three-dimensional structure of the MMP-9/TIMP-1 complex, overview
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3,7,8-tetrachlorodibenzo-p-dioxin
-
both MMP-9 mRNA expression and enzymatic activity are gradually increased with the concentration increase of 2,3,7,8-tetrachlorodibenzo-p-dioxin in media and these changes can be reversed by resveratrol treatment in a dose-dependent manner, c-Jun mediates 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced MMP-9 expression and activity
4-amino phenylmercuric acetate
-
pro-MMP-9 is activated with 1 mM 4-amino phenylmercuric acetate in assay buffer at 37C for 1.5 h
4-aminophenylmercuric acetate
acrolein
-
activates MMP-9 via EGFR/MAPK signaling and stimulation of pro-MMP-9 cleavage
aminophenylmercuric acetate
-
-
APMA
-
-
cathepsin G
-
MMP9 is released in an inactive form and requires proteolytic activation, cathepsin G mediates activation of pro-matrix metalloproteinase 9
-
chymase
-
chymase converts promatrix metalloproteinase-9 to matrix metalloproteinase-9
-
fibronectin
-
fibronectin enhances, in a PKC-dependent manner, the net activity of MMP-9, but not its expression
-
homocysteine
-
homocysteine activates matrix metalloproteinase-9, muscimol ameliorates the homocysteine-mediated MMP-9 activation
interleukin-1
-
-
-
Interleukin-1beta
-
-
-
lipopolysaccharide
-
lipopolysaccharide challenge (0.001 mg/ml) increases the protein level of MMP-9 and induces the activity of MMP-9 in H9c2 cardiomyoblasts through ERK1/2 signaling pathway
matrix metalloproteinase 3
-
converts inactive pro-MMP-9 to active MMP-9
-
matrix metalloproteinase-7
-
MMP-9 is activated by matrix metalloproteinase-7
-
phorbol 12-myristate 13-acetate
platelet-activating factor
-
i.e. 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine, induces MMP-9 enzyme expression, and increases enzyme content and activity
tumor necrosis factor-alpha
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0015
65000 MW form of vitronectin
-
-
-
0.00422 - 0.00496
N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-7-methoxycoumarin-4-yl acetyl
0.8
SGFGSRYLTA
-
pH 7.5, 37C, peptide A19
3.7
SGKGPRQITA
-
pH 7.5, 37C, peptide C15
1.4
SGKIPRRLTA
-
pH 7.5, 37C, peptide A11m1
4.7
SGKIPRTATA
-
pH 7.5, 37C, peptide A11m2
1.3
SGKIPRTLTA
-
pH 7.5, 37C, peptide A11
2.4
SGLKALMITA
-
pH 7.5, 37C, peptide A7
13.5
SGLRPAKSTA
-
pH 7.5, 37C, peptide A18
0.5
SGPLFYSVTA
-
pH 7.5, 37C, peptide A10
2.9
SGPRAVSTTA
-
pH 7.5, 37C, peptide A6
1
SGQPHYLTTA
-
pH 7.5, 37C, peptide B1
0.000013 - 0.000025
stromelysin1
0.0013
vitronectin
-
75000 MW form
-
additional information
additional information
-
detailed binding kinetics
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04 - 8.67
(7-methoxycoumaryl-4yl)acetylPLGLA2pr(2,4-dinitrophenol)-AR-NH2
0.0005
65000 MW form of vitronectin
Homo sapiens
-
-
-
0.000333
75000 MW form of vitronectin
Homo sapiens
-
-
-
11
SGFGSRYLTA
Mus musculus
-
pH 7.5, 37C, peptide A19
703
SGKGPRQITA
Mus musculus
-
pH 7.5, 37C, peptide C15
224
SGKIPRRLTA
Mus musculus
-
pH 7.5, 37C, peptide A11m1
9.4
SGKIPRTATA
Mus musculus
-
pH 7.5, 37C, peptide A11m2
86.4
SGKIPRTLTA
Mus musculus
-
pH 7.5, 37C, peptide A11
30
SGLKALMITA
Mus musculus
-
pH 7.5, 37C, peptide A7
78
SGLRPAKSTA
Mus musculus
-
pH 7.5, 37C, peptide A18
20
SGPLFYSVTA
Mus musculus
-
pH 7.5, 37C, peptide A10
178
SGPRAVSTTA
Mus musculus
-
pH 7.5, 37C, peptide A6
9
SGQPHYLTTA
Mus musculus
-
pH 7.5, 37C, peptide B1
0.00041 - 0.0019
stromelysin 1
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00000713 - 0.00000817
TIMP-1
0.0000259 - 0.0000361
TIMP-2
additional information
additional information
-
detailed binding kinetics
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000045
(2E)-3-(N-hydroxycarbamoyl)-2-(3-phenylpropylidene)propionyl-L-tryptophan-N-methylamide
Homo sapiens
-
-
0.01
(2E)-3-(N-hydroxycarbamoyl)-2-heptylidenepropionyl-L-tryptophan-N-methylamide
Homo sapiens
-
above
0.000017
(2E)-3-(N-hydroxycarbamoyl)-2-isopropionyl-L-tryptophan-N-methylamide
Homo sapiens
-
-
0.000038
(2E)-3-(N-hydroxycarbamoyl)-2-[(2E)-3-phenylprop-2-en-1-ylidene]propionyl-L-tryptophan-N-methylamide
Homo sapiens
-
-
0.000974
(2E)-3-(N-hydroxycarbamoyl)-2-[(2E)-but-2-en-1-ylidene]propionyl-L-tryptophan-N-methylamide
Homo sapiens
-
-
0.0000005
(R)-2-(N-((6-fluoropyridin-3-yl)methyl)-4-methoxyphenyl-sulfonamido)-N-hydroxy-3-methylbutanamide
Mus musculus
-
-
0.017 - 0.0209
3',4',5,6,7,8-hexamethoxyflavone
0.0108 - 0.0123
3',4'-dihydroxy-5,6,7,8-tetramethoxyflavone
0.0164 - 0.0169
3'-hydroxy-4',5,6,7,8-pentamethoxyflavone
0.0037 - 0.0055
4'-hydroxy-3',5,6,7,8-pentamethoxyflavone
0.000026
5-(1,5-dihydroxypentan-3-yl)-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.000145
5-(4-hydroxybutan-2-yl)-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.000104
5-(4-phenoxyphenyl)-5-(3-(1-nitrooxy-methyl)piperidin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.000152
5-(4-phenoxyphenyl)-5-(4-(1-nitrooxy-ethyl)piperidin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.000092
5-(4-phenoxyphenyl)-5-(4-(2-nitrooxy-ethyl)piperazin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.000202
5-(4-phenoxyphenyl)-5-(4-(2-nitrooxy-ethyl)piperidin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.000091
5-(4-phenoxyphenyl)-5-(bis-(2-nitrooxy-ethyl)-amino)pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.000048
5-(4-phenoxyphenyl)-5-(methyl-(2-nitrooxy-ethyl)-amino)-pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.0000062
5-[3-(hydroxymethyl)piperidin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.0000081
5-[4-(2-hydroxyethyl)piperazin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.000012
5-[4-(2-hydroxyethyl)piperidin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.000097
5-[4-(hydroxymethyl)piperidin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
Homo sapiens
-
pH 7.5, 37C
0.000006
CGS 27023A
Mus musculus
-
-
0.0000002 - 0.0000005
ilomastat
additional information
additional information
Homo sapiens
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
-
assay at
8
-
assay at
8.3
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 30
-
assay at
25 - 37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
increased enzyme expression, the positive ratio of MMP-9 tends to increase from Grade II to Grade IV glioma and strong positive MMP-9 staining is more frequently detected in high-grade glioma, association between matrix metalloproteinase-9 expression and survival of patients
Manually annotated by BRENDA team
-
MMP-9 secretion
Manually annotated by BRENDA team
-
in the walls
Manually annotated by BRENDA team
-
bone marrow-derived cells are the major source of MMP-9 in the ischemic brain
Manually annotated by BRENDA team
-
glial-neuronal coculture
Manually annotated by BRENDA team
-
localized mainly in the cytoplasm of neoplastic astrocytes
Manually annotated by BRENDA team
-
expression and activity of MMP-9 during premature rupture of chorioamniotic membranes, secretion of proMMP-9 from the amniochorion after application of lipopolysacchride to either sides of the membrane is increased, overview
Manually annotated by BRENDA team
-
coronary arteritis is induced by Lactobacillus casei cell wall extract injection
Manually annotated by BRENDA team
-
demineralized dentin matrix, extraction is best at acidic conditions of pH 2-3 compared to pH 7.4-EDTA-containing extracts
Manually annotated by BRENDA team
-
colon adenocarcinoma cell line
Manually annotated by BRENDA team