Information on EC 3.4.24.32 - beta-Lytic metalloendopeptidase

New: Word Map on EC 3.4.24.32
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY hide
3.4.24.32
-
RECOMMENDED NAME
GeneOntology No.
beta-Lytic metalloendopeptidase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of N-acetylmuramoyl-/-Ala, and of the insulin B chain at Gly23-/-Phe > Val18-/-Cya
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
37288-92-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
M497-1
-
-
Manually annotated by BRENDA team
three isozymes
UniProt
Manually annotated by BRENDA team
three isozymes
UniProt
Manually annotated by BRENDA team
strain 495
-
-
Manually annotated by BRENDA team
strain AL-1
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-(2-Furylacryloyl)-Gly-Leu amide + H2O
?
show the reaction diagram
-
-
-
-
-
Azocoll + H2O
Hydrolyzed azocoll
show the reaction diagram
Bacterial cell walls + H2O
Hydrolyzed bacterial cell walls
show the reaction diagram
Benzyloxycarbonyl-Gly-Phe amide + H2O
?
show the reaction diagram
-
-
-
-
-
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
hydrolyzed casein
show the reaction diagram
-
limited activity
-
-
-
DYMGWMDF-NH2 + H2O
DYMG + WMDF-NH2
show the reaction diagram
-
-
-
?
elastin-orcein + H2O
?
show the reaction diagram
-
-
-
-
?
Elastin-orcein + H2O
Hydrolyzed elastin-orcein
show the reaction diagram
-
-
-
-
-
furylacryloyl-Gly-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
GNLWATGHFM-NH2 + H2O
GNLWATG + HFM-NH2
show the reaction diagram
-
-
-
?
Insulin B-chain + H2O
?
show the reaction diagram
-
-
-
-
?
Insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
KFIGLM-NH2 + H2O
KFIG + Leu-Met-NH2
show the reaction diagram
-
-
-
?
L-pyroglutamyl-HWSYGLRPG-NH2 + H2O
L-pyroglutamyl-HWSYG + LRPG-NH2
show the reaction diagram
-
-
-
?
L-pyroglutamyl-LM-NH2 + H2O
?
show the reaction diagram
-
-
-
?
L-pyroglutamyl-QRLGNQWAVGHLM-NH2 + H2O
L-pyroglutamyl-QRLG + NQWAVGHLM-NH2
show the reaction diagram
-
-
-
?
L-pyroglutamyl-VPQWAVGHFM-NH2 + H2O
L-pyroglutamyl-VPQWAVG + HFM-NH2
show the reaction diagram
-
-
-
?
pentaglycine + H2O
?
show the reaction diagram
peptidoglycan + H2O
?
show the reaction diagram
RPPGFSPFR + H2O
RPPG + FSPFR
show the reaction diagram
-
-
-
?
WAGGDASGE + H2O
WAGG + DASGE
show the reaction diagram
-
-
-
?
WAGGNASGE + H2O
WAGG + NASGE
show the reaction diagram
-
-
-
?
Z-Gly-Phe-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zinc
-
Lysobacter enzymogenes enzyme contains a zinc atom
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2-mercaptoethanol
-
66% inhibition
additional information
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 9
-
bacteriolysis, Lysobacter enzymogenes
10
-
bacterial substrates
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10.5
-
exhibits higher bacteriolytic activity at pH 10.0 than at pH 7.0-9.0
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.3
sequence calculation
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19280
-
acetonitrile-inactivated enzyme, ion spray mass spectrometry; calculated from the amino acid sequence deduced from the nucleotide sequence of the cloned blp gene
19350
-
active enzyme, ion spray mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Lysobacter enzymogenes
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
1 h, 95% loss of activity
31199
6.5 - 9.5
-
45°C, 1 h, maximal stability
31199
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
0.02 M Tris-HCl, pH 9.0, 13% loss of activity after 8 h
40 - 50
-
0.02 M Tris-HCl, pH 9.0, 70% loss of activity after 8 h
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 0.02 M Tris-HCl, pH 9.0, stable for at least 8 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial purification from an extracellular sample by anion exchange chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
additional information