Information on EC 3.4.24.32 - beta-Lytic metalloendopeptidase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
3.4.24.32
-
RECOMMENDED NAME
GeneOntology No.
beta-Lytic metalloendopeptidase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cleavage of N-acetylmuramoyl-/-Ala, and of the insulin B chain at Gly23-/-Phe > Val18-/-Cya
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Achromopeptidase component
-
-
-
-
Achromopeptidase component
-
-
beta-lytic metalloproteinase
-
-
beta-Lytic protease
-
-
-
-
beta-Lytic protease
-
-
beta-Lytic protease
-
-
EC 3.4.99.13
-
formerly
EC3.4.24.4
-
formerly included in
Myxobacter AL-1 proteinase I
-
formerly EC 3.4.99.29
Myxobacter beta-lytic proteinase
-
-
-
-
Myxobacter beta-lytic proteinase
-
-
Myxobacter495 beta-lytic proteinase
-
-
-
-
Myxobacter495 beta-lytic proteinase
-
-
Myxobacterium sorangium beta-lytic proteinase
-
-
-
-
Myxobacterium sorangium beta-lytic proteinase
-
-
Proteinase, beta lytic metallo-
-
-
-
-
Proteinase, Myxobacterium sorangium beta-lytic
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37288-92-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Achromobacter lyticus M497-1
M497-1
-
-
Manually annotated by BRENDA team
unidentified myxobacterium 495
strain 495
-
-
Manually annotated by BRENDA team
unidentified myxobacterium AL-1
strain AL-1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-(2-Furylacryloyl)-Gly-Leu amide + H2O
?
show the reaction diagram
-
-
-
-
-
Azocoll + H2O
Hydrolyzed azocoll
show the reaction diagram
unidentified myxobacterium, unidentified myxobacterium AL-1
-
-
-
-
-
Bacterial cell walls + H2O
Hydrolyzed bacterial cell walls
show the reaction diagram
-
cleaves specific peptide bonds within the cell wall peptidoglycan network
-
-
-
Bacterial cell walls + H2O
Hydrolyzed bacterial cell walls
show the reaction diagram
-
Arthrobacter crystallopoietes cells walls
-
-
-
Bacterial cell walls + H2O
Hydrolyzed bacterial cell walls
show the reaction diagram
-
Lysobacter enzymogenes enzyme: Arthrobacter globiformis cells, Micrococcus lysodeikticus cells
-
-
-
Bacterial cell walls + H2O
Hydrolyzed bacterial cell walls
show the reaction diagram
unidentified myxobacterium AL-1
-
Arthrobacter crystallopoietes cells walls
-
-
-
Benzyloxycarbonyl-Gly-Phe amide + H2O
?
show the reaction diagram
-
-
-
-
-
casein + H2O
hydrolyzed casein
show the reaction diagram
-
limited activity
-
-
-
casein + H2O
?
show the reaction diagram
-
-
-
-
?
DYMGWMDF-NH2 + H2O
DYMG + WMDF-NH2
show the reaction diagram
-
-
-
?
Elastin-orcein + H2O
Hydrolyzed elastin-orcein
show the reaction diagram
-
-
-
-
-
elastin-orcein + H2O
?
show the reaction diagram
-
-
-
-
?
furylacryloyl-Gly-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
GNLWATGHFM-NH2 + H2O
GNLWATG + HFM-NH2
show the reaction diagram
-
-
-
?
Insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
-
does not act on A-chain of oxidized insulin, it cleaves the B-chain readily at Gly23-Phe24 and more slowly at Val18-Cys19-SO3H
-
-
Insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
unidentified myxobacterium, unidentified myxobacterium AL-1
-
hydrolysis of Ala-Leu bond
-
-
Insulin B-chain + H2O
?
show the reaction diagram
-
-
-
-
?
KFIGLM-NH2 + H2O
KFIG + Leu-Met-NH2
show the reaction diagram
-
-
-
?
L-pyroglutamyl-HWSYGLRPG-NH2 + H2O
L-pyroglutamyl-HWSYG + LRPG-NH2
show the reaction diagram
-
-
-
?
L-pyroglutamyl-LM-NH2 + H2O
?
show the reaction diagram
-
-
-
?
L-pyroglutamyl-QRLGNQWAVGHLM-NH2 + H2O
L-pyroglutamyl-QRLG + NQWAVGHLM-NH2
show the reaction diagram
-
-
-
?
pentaglycine + H2O
?
show the reaction diagram
unidentified myxobacterium, unidentified myxobacterium AL-1
-
-
-
-
-
peptidoglycan + H2O
?
show the reaction diagram
-
-
-
-
?
RPPGFSPFR + H2O
RPPG + FSPFR
show the reaction diagram
-
-
-
?
WAGGDASGE + H2O
WAGG + DASGE
show the reaction diagram
-
-
-
?
WAGGNASGE + H2O
WAGG + NASGE
show the reaction diagram
-
-
-
?
Z-Gly-Phe-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
L-pyroglutamyl-VPQWAVGHFM-NH2 + H2O
L-pyroglutamyl-VPQWAVG + HFM-NH2
show the reaction diagram
-
-
-
?
additional information
?
-
-
affinity towards peptide bonds formed by at least one hydrophobic amino acid in a structure at least as large as a tetrapeptide
-
-
-
additional information
?
-
-
the primary biological role is the defense against bacteria in the environment, in particular against species of Staphylococcus or closely related organisms
-
-
-
additional information
?
-
-
no cleavage of Z-Gly-Phe
-
-
?
additional information
?
-
-
the enzyme only cleaves Gly-X bonds and favors hydrophobic or apolar residues to either side, it does not hydrolyze bonds with charged amino acids or proline adjacent, cleaves D-Ala-Gly and D-Ala-Ala bonds in the linkage between peptide subunit and the interpeptide bridge and also the Gly-Gly bond in the Staphylococcus aureus interpeptide bridge
-
-
-
additional information
?
-
unidentified myxobacterium AL-1
-
affinity towards peptide bonds formed by at least one hydrophobic amino acid in a structure at least as large as a tetrapeptide
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
the primary biological role is the defense against bacteria in the environment, in particular against species of Staphylococcus or closely related organisms
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Zinc
-
Lysobacter enzymogenes enzyme contains a zinc atom
Zn2+
-
one zinc atom detected in the molecule by ion-spray mass spectrometry
Zn2+
-
required for activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
-
1,10-phenanthroline
-
90% inhibition
1,10-phenantroline
-
-
2-mercaptoethanol
-
66% inhibition
additional information
-
citrate; dinitrofluorobenzene; EDTA; iodoacetic acid; not: PCMB
-
additional information
-
diisopropylfluorophosphate
-
additional information
-
only 2% inhibition with EDTA, no inhibition with diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, iodoacetate, phosphoramidon, N-chloroacetyl-N-hydroxy-leucyl-Ala-Gly-NH2, chymostatin, elastinal, leupeptin, and pepstatin
-
additional information
-
insensitive to DFP
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
-
3-(2-furylacryloyl)-Gly-Leu-amide, Lysobacter enzymogenes
6.5
-
-
for FA-Gly-Leu-NH2 hydrolysis
8.5
9
-
bacteriolysis, Lysobacter enzymogenes
9
-
-
cell wall lysis, azocoll
9
-
-
peptide substrates
9
-
-
for bacteriolysis
10
-
-
bacterial substrates
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
10.5
-
exhibits higher bacteriolytic activity at pH 10.0 than at pH 7.0-9.0
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
19100
-
-
Lysobacter enzymogenes, calculated from amino acid sequence
19280
-
-
acetonitrile-inactivated enzyme, ion spray mass spectrometry; calculated from the amino acid sequence deduced from the nucleotide sequence of the cloned blp gene
19350
-
-
active enzyme, ion spray mass spectrometry
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 22000 Achromobacter lyticus, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Lysobacter enzymogenes
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3
-
-
1 h, 95% loss of activity
6.5
9.5
-
45°C, 1 h, maximal stability
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35
-
-
0.02 M Tris-HCl, pH 9.0, 13% loss of activity after 8 h
40
50
-
0.02 M Tris-HCl, pH 9.0, 70% loss of activity after 8 h
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4°C, 0.02 M Tris-HCl, pH 9.0, stable for at least 8 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
ability to lyse Micrococcus luteus and Staphylococcus aureus cells
additional information
-
ability to lyse Arthrobacter globiformis, Micrococcus luteus and Staphylococcus aureus cells, inhibits growth of sensitive organisms and may potentially serve as an antimicrobial agent