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Information on EC 3.4.24.20 - peptidyl-Lys metalloendopeptidase and Organism(s) Grifola frondosa and UniProt Accession P81054

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.20 peptidyl-Lys metalloendopeptidase
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This record set is specific for:
Grifola frondosa
UNIPROT: P81054 not found.
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Word Map
The taxonomic range for the selected organisms is: Grifola frondosa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Preferential cleavage in proteins: -Xaa-/-Lys- (in which Xaa may be Pro)
Synonyms
lys-n, gfmep, pomep, peptidyl-lys metalloendopeptidase, peptidyl-lys metallopeptidase, am-lysn, aspzincin metalloendopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Armillaria mellea neutral proteinase
-
-
-
-
aspzincin metalloendopeptidase
-
-
GFMEP
MEP
-
-
-
-
peptidyl-Lys metalloendopeptidase
-
-
Peptidyllysine metalloproteinase
-
-
-
-
POMEP
-
-
-
-
Proteinase, peptidyllysine metallo-
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
65979-41-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl-ADKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-APKLSW + H2O
2,4-dinitrophenyl-Ala-Pro + KLSW
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl-APKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-ARKLSW + H2O
2,4-dinitrophenyl-Ala-Arg + KLSW
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl-ARKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-ATKDSW + H2O
2,4-dinitrophenyl-Ala-Thr + KDSW
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl-ATKDSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-ATKLSTSW + H2O
2,4-dinitrophenyl-Ala-Thr + KLSTSW
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl-ATKLSTSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-ATKLSW + H2O
2,4-dinitrophenyl-Ala-Thr + KLSW
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl-ATKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-ATKRSW + H2O
2,4-dinitrophenyl-Ala-Thr + KRSW
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl-ATKRSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-DADKLSW + H2O
2,4-dinitrophenyl-DAD + KLSW
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl-DADKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-DATKLSW + H2O
2,4-dinitrophenyl-DAT + KLSW
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl-DATKLSW + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-Ser-Thr-Ala-Thr-Lys-Leu-Ser-Trp + H2O
?
show the reaction diagram
fluorescent peptide substrate
-
?
2,4-dinitrophenyl-STATKLSW + H2O
2,4-dinitrophenyl-STAT + KLSW
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl-STATKLSW + H2O
?
show the reaction diagram
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
Azocoll + H2O
?
show the reaction diagram
-
general protease substrate
-
-
?
Bovine serum albumin + H2O
?
show the reaction diagram
-
-
-
-
?
Congo red elastin + H2O
?
show the reaction diagram
-
-
-
-
?
Equine heart cytochrome c + H2O
?
show the reaction diagram
-
cleaves-X-Lys bonds
-
-
?
protamine sulfate + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
zinc-metalloendopetidase
Ca2+
-
activation, zinc-depleted apoenzyme
Co2+
-
activation, zinc-depleted apoenzyme
Mn2+
-
activation, 0.1 mM, native enzyme and zinc-depleted apoenzyme
Zn2+
-
contains 2 zinc ligands
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-dinitrophenyl-Ala-Thr-D-lysine-Leu-Ser-Try
-
1,10-phenanthroline
-
strong, caseinolytic activity
2,2'-bipyridine
-
strong, caseinolytic activity
2-mercaptoethanol
-
weak, caseinolytic activity
6-aminohexanoic acid
-
caseinolytic activity
agmatine
-
caseinolytic activity
antipain
-
weak, caseinolytic activity
Ba2+
-
1 mM, weak, caseinolytic activity
Ca2+
-
1 mM, weak, caseinolytic activity
Co2+
-
0.1 mM, caseinolytic activity
Cu2+
-
1 mM, caseinolytic activity
dithiothreitol
DL-homoarginine
-
caseinolytic activity
Hg2+
-
strong, caseinolytic activity
L-aminobutyric acid
-
caseinolytic activity
L-arginine
-
caseinolytic activity
L-lysine
-
caseinolytic activity
L-Lysine ethylester
-
caseinolytic activity
L-ornithine
-
caseinolytic activity
Mg2+
-
1 mM, weak, caseinolytic activity
p-chloromercuribenzoate
-
not
phosphoramidon
-
weak, caseinolytic activity
tosyl-Lys chloromethyl ketone
-
not
tosyl-Phe chloromethyl ketone
-
weak, caseinolytic activity
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0116
2,4-dinitrophenyl-ADKLSW
pH 9.5, 37°C
0.0111
2,4-dinitrophenyl-APKLSW
pH 9.5, 37°C
0.0152
2,4-dinitrophenyl-ARKLSW
pH 9.5, 37°C
0.0249
2,4-dinitrophenyl-ATKDSW
pH 9.5, 37°C
0.0133
2,4-dinitrophenyl-ATKLSTSW
pH 9.5, 37°C
0.0121
2,4-dinitrophenyl-ATKLSW
pH 9.5, 37°C
0.0177
2,4-dinitrophenyl-ATKRSW
pH 9.5, 37°C
0.0155
2,4-dinitrophenyl-DADKLSW
pH 9.5, 37°C
0.0088
2,4-dinitrophenyl-DATKLSW
pH 9.5, 37°C
0.0114
2,4-dinitrophenyl-STATKLSW
pH 9.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.3
2,4-dinitrophenyl-ADKLSW
pH 9.5, 37°C
5.5
2,4-dinitrophenyl-APKLSW
pH 9.5, 37°C
54.7
2,4-dinitrophenyl-ARKLSW
pH 9.5, 37°C
0.25
2,4-dinitrophenyl-ATKDSW
pH 9.5, 37°C
0.99 - 17.6
2,4-dinitrophenyl-ATKLSTSW
3
2,4-dinitrophenyl-ATKLSW
pH 9.5, 37°C
3.7
2,4-dinitrophenyl-ATKRSW
pH 9.5, 37°C
0.12
2,4-dinitrophenyl-DADKLSW
pH 9.5, 37°C
5.1
2,4-dinitrophenyl-DATKLSW
pH 9.5, 37°C
21
2,4-dinitrophenyl-STATKLSW
pH 9.5, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0399
2,4-dinitrophenyl-Ala-Thr-D-lysine-Leu-Ser-Try
pH 9.5, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
congo red elastin as substrate
9.5
-
azocoll or azocasein as substrate
additional information
-
pI: 7.46
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10.5
exhibits more than 50% of the maximal activity over this range
6 - 10.5
-
more than 50% of maximal activity at pH 6 and 10.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
edible mushroom
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PLMP_GRIFR
348
0
36879
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
x * 20000, Grifola frondosa, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 20000, Grifola frondosa, SDS-PAGE
monomer
-
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
solved in 4 crystal forms, triclinic space group P1, unit cell parameters a : 30.8 A, b : 40.5 A, c : 30.5 A, monoclinic space group C2, unit cell parameters a : 43.6 A, b : 41.9 A, c : 76.9 A, tetragonal space group P4(3), unit cell parameters a : b : 30.2 A, c : 308.0 A, and hexangonal P6(5)22, unit cell parameters a : b 58.7 A, c : 145.2 A, hanging-drop vapour-diffusion method
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
-
18 h, about 20% loss of activity at 4°C
31047
7.2
-
3 h, stable at 70°C and below, 40% loss of activity at 80°C, inactivation within 30 min at 100°C
31047
9
-
3 h, about 30%, 55% and 90% loss of activity at 50°C, 60°C and 70°C, respectively
31047
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
30 min, inactivation
50
-
3 h, about 30% loss of activity at pH 9
60
-
3 h, about 55% loss of activity at pH 9
80
-
3 h, 40% loss of activity at pH 7.2, 30 min, about 90% loss of activity with Mn2+ instead of zinc
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-40°C, pH 7, at least a year
-
4°C, in various buffers, pH 5-10, about 20% loss of activity within 18 h
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molecular biology
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nonaka, T.; Ishikawa, H.; Tsumuraya, Y.; Hashimoto, Y.; Dohmae, N.; Takio, K.
Characterization of a thermostable lysine-specific metalloendopeptidase from the fruiting bodies of a basidiomycete, Grifola frondosa
J. Biochem.
118
1014-1020
1995
Grifola frondosa
Manually annotated by BRENDA team
Hori, T.; Kumasaka, T.; Yamamoto, M.; Nonaka, T.; Tanaka, N.; Hashimoto, Y.; Ueki, T.; Takio, K.
Structure of a new 'aspzincin' metalloendopeptidase from Grifola frondosa: implications for the catalytic mechanism and substrate specificity based on several different crystal forms
Acta Crystallogr. Sect. D
D57
361-368
2001
Grifola frondosa
Manually annotated by BRENDA team
Nonaka, T.; Hashimoto, Y.; Takio, K.
Kinetic characterization of lysine-specific metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus fruiting bodies
J. Biochem.
124
157-162
1998
Grifola frondosa (P81054), Grifola frondosa, Pleurotus ostreatus (P81055), Pleurotus ostreatus
Manually annotated by BRENDA team
Rao, K.C.; Carruth, R.T.; Miyagi, M.
Proteolytic 18O labeling by peptidyl-Lys metalloendopeptidase for comparative proteomics
J. Proteome Res.
4
507-514
2005
Grifola frondosa
Manually annotated by BRENDA team
Rao, K.C.; Palamalai, V.; Dunlevy, J.R.; Miyagi, M.
Peptidyl-Lys metalloendopeptidase-catalyzed 18O labeling for comparative proteomics: application to cytokine/lipolysaccharide-treated human retinal pigment epithelium cell line
Mol. Cell. Proteomics
4
1550-1557
2005
Grifola frondosa
Manually annotated by BRENDA team
Kishimoto, T.; Kondo, J.; Takai-Igarashi, T.; Tanaka, H.
Accurate mass comparison coupled with two endopeptidases enables identification of protein termini
Proteomics
11
485-489
2011
Grifola frondosa
Manually annotated by BRENDA team